Metalloproteins in the Biology of Heterocysts

Pernil, Rafael; Schleiff, Enrico

doi:10.3390/life9020032

Cited by 34 publications

(25 citation statements)

References 459 publications

(997 reference statements)

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“…The ability to use solar energy to split water with only H2 and O2 as the products is an ideal, carbon-neutral energy goal with considerable promise. One challenge to such a device is to somehow protect the hydrogenases that are sensitive to O2 by some sort of sequestration similar to how a similarly O2 sensitive enzyme, nitrogenases, are separated from PSII O2 evolution in heterocysts [111,112].…”

Section: Emerging Technologies For Bioengineering Hydrogen Evolutionmentioning

confidence: 99%

Green Catalysts: Applied and Synthetic Photosynthesis

Teodor¹,

Sherman²,

Ison³

et al. 2020

Preprint

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The biological process of photosynthesis was critical in catalyzing the oxygenation of Earth’s atmosphere 2.5 billion years ago, changing the course of development of life on Earth. Recently, the fields of applied and synthetic photosynthesis have utilized the light-driven protein-pigment supercomplexes central to photosynthesis for the photocatalytic production of fuel and other various valuable products. The reaction center Photosystem I is of particular interest in applied photosynthesis due to its high stability post-purification, non-geopolitical limitation, and its ability to generate the greatest reducing power found in Nature. These remarkable properties have been harnessed for the photocatalytic production of a number of valuable products in the applied photosynthesis research field. These primarily include photocurrents and molecular hydrogen as fuels. The use of artificial reaction centers to generate substrates and reducing equivalents to drive non-photoactive enzymes for valuable product generation has been a long-standing area of interest of the synthetic photosynthesis research field. In this review, we cover advances in these areas and further speculate synthetic and applied photosynthesis as photocatalysts for the generation of valuable products.

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Section: Emerging Technologies For Bioengineering Hydrogen Evolutionmentioning

confidence: 99%

Green Catalysts: Applied and Synthetic Photosynthesis

Teodor¹,

Sherman²,

Ison³

et al. 2020

Preprint

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“…Furthermore, FurA is able to bind 2-oxoglutarate (2-OG), a signal molecule that informs about the C/N balance in the cell (Guio et al, 2020). In the last years, some articles point out a strong cross-talk between iron deficiency, oxidative stress response and nitrogen metabolism (Pernil and Schleiff, 2019). It is well known that in Anabaena sp.…”

Section: Introductionmentioning

confidence: 99%

FurC (PerR) from Anabaena sp. PCC7120: a versatile transcriptional regulator engaged in the regulatory network of heterocyst development and nitrogen fixation

Sarasa‐Buisan

Guío

Broset

et al. 2021

Environmental Microbiology

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FurC (PerR) from Anabaena sp. PCC7120 was previously described as a key transcriptional regulator involved in setting off the oxidative stress response. In the last years, the cross-talk between oxidative stress, iron homeostasis and nitrogen metabolism is becoming more and more evident. In this work, the transcriptome of a furC-overexpressing strain was compared with that of a wild-type strain under both standard and nitrogen-deficiency conditions. The results showed that the overexpression of furC deregulates genes involved in several categories standing out photosynthesis, iron transport and nitrogen metabolism. The novel FurC-direct targets included some regulatory elements that control heterocyst development (hetZ and asr1734), genes directly involved in the heterocyst envelope formation (devBCA and hepC) and genes which participate in the nitrogen fixation process (nifHDK and nifH2, rbrA rubrerythrin and xisHI excisionase). Likewise, furC overexpression notably impacts the mRNA levels of patA encoding a key protein in the heterocyst pattern formation. The relevance of FurC in these processes is bringing out by the fact that the overexpression of furC impairs heterocyst development and cell growth under nitrogen stepdown conditions. In summary, this work reveals a new player in the complex regulatory network of heterocyst formation and nitrogen fixation.

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“…Metalloproteins and metalloenzymes play important roles in biological systems, including electron transfer, O 2 binding and delivery, and catalysis, etc. [1,2,3,4,5,6,7,8,9,10,11]. Despite the functional diversity, the cofactor or prosthetic group of native metalloproteins/metalloenzymes are made of about 14 metal ions, and several types of metal complexes (such as heme) or metal clusters (such as iron–sulfur clusters) [4,5,10], which may limit the functionalities of native metalloenzymes.…”

Section: Introductionmentioning

confidence: 99%

Rational Design of Artificial Metalloproteins and Metalloenzymes with Metal Clusters

Lin

2019

Molecules

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Metalloproteins and metalloenzymes play important roles in biological systems by using the limited metal ions, complexes, and clusters that are associated with the protein matrix. The design of artificial metalloproteins and metalloenzymes not only reveals the structure and function relationship of natural proteins, but also enables the synthesis of artificial proteins and enzymes with improved properties and functions. Acknowledging the progress in rational design from single to multiple active sites, this review focuses on recent achievements in the design of artificial metalloproteins and metalloenzymes with metal clusters, including zinc clusters, cadmium clusters, iron–sulfur clusters, and copper–sulfur clusters, as well as noble metal clusters and others. These metal clusters were designed in both native and de novo protein scaffolds for structural roles, electron transfer, or catalysis. Some synthetic metal clusters as functional models of native enzymes are also discussed. These achievements provide valuable insights for deep understanding of the natural proteins and enzymes, and practical clues for the further design of artificial enzymes with functions comparable or even beyond those of natural counterparts.

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Metalloproteins in the Biology of Heterocysts

Cited by 34 publications

References 459 publications

Green Catalysts: Applied and Synthetic Photosynthesis

Green Catalysts: Applied and Synthetic Photosynthesis

FurC (PerR) from Anabaena sp. PCC7120: a versatile transcriptional regulator engaged in the regulatory network of heterocyst development and nitrogen fixation

Rational Design of Artificial Metalloproteins and Metalloenzymes with Metal Clusters

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