Metalloproteinases and TIMPs production by AIDS-Kaposi sarcoma derived cells

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“…Given our double goal in this study to characterize Kaposi spindle like cells in order to check potential inhibitors for treatment of the corresponding disease, and to define maintenance conditions for Cowdria ruminantium infected BUEC cells in order to propose an appropriate vaccine production process -the present approach has been quite rewarding. Kaposi Sarcoma (KS) cells that we isolated from AIDS patients biopsies, expressed Factor VIII, indicating their endothelial origin, and as BUEC, expressed 92 kD MMPase at high level when stimulated either by PMA, TNF, or by both agents as indicated previously (Blankaert et al, 1994). We showed here that TGF and activated conditioned medium reduced the expression of 92 kD MMPase, appearing therefore as a potential cell mobility inhibitor of interest for KS treatment.…”

“…Kaposi sarcoma cells. KS cell cultures were established as previously described (Blankaert et al, 1994), and as it will be reported more extensively elsewhere. In brief, biopsies of KS lesions minced into small pieces were treated for 90 minutes at 37 C with Trypsin (0.3% -1% EDTA) or collagenase (1 mg/ml from Clostridium histolyticum).…”

Control of 92 kDa Collagenase Secretion in Mammalian Cells by Modulation of AP-1 Activity: an Experimentally based Theoretical Study

“…Collagenases attract much interest, as they play a pivotal role in all processes involving cell migration through the organism, such as angiogenesis (Gross et al, 1983;Blankaert et al, 1992Blankaert et al, , 1994, arthritis (Clark et al, 1993) or metastasis dissemination (Sato & Seiki, 1988;Mignatti & Rifkin, 1993). In particular, the 92 kDa gelatinase shows good correlation with tumoral invasiveness.…”