Metalloimmunoassay

Cais, Michael; Dani, S.; Eden, Yehudith; Gandolfi, Ottavio; HORN, M.; ISAACS, E. E.; Josephy, Y.; SAAR, Y.; Slovin, Eliot Mark; Snarsky, L.

doi:10.1038/270534a0

Cited by 102 publications

(35 citation statements)

References 3 publications

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“…These advantages make it a perfect candidate for building hand-held, point of care medical devices. The first assay of this type was the metalloimmunoassay, which used metal atoms in the form of their organometallic or coordination complexes as labeling agent [1]. The introduction of metal nanoparticles as biotags, with their unique chemical and electrochemical properties combined with the simplicity of attaching them to antibodies, revolutionized metalloimmunoassay, especially with respect to achieving lower detection limits.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Dissolution of Silver Nanoparticles and Its Application in Metalloimmunoassay

2010

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This work demonstrates the use of silver nanoparticles as a simple electrochemical biolabel to induce 10 6 signal enhancement. We propose a mechanism of measuring the silver nanoparticles on a specific screen-printed planar carbon electrode, without the requirement for the harsh oxidant or toxic reagents described in prior-art for gold sol methods. The possible measurement process was validated with orthogonal techniques such as UV/Vis spectroscopy, dynamic light scattering and by anodic stripping voltammetry (ASV). The findings were utilized to develop a novel electrochemical sandwich immunoassay where the analyte concentration is directly proportional to ASV oxidation peak of silver. This technique in the future is envisaged to form the foundation of a generic Point of Care platform. The assay was applied to cardiac marker: myoglobin with detection limit of 3 ng/mL.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Dissolution of Silver Nanoparticles and Its Application in Metalloimmunoassay

2010

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“…Ferrocenhaltige Ligandensysteme verdienen Beachtung wegen Nutzung der Ferrocenuntereinheit als Redoxschalter bei weiterer Komplexierung [18,19], als Elektrodenmaterial [20] und für Immunoassaytechniken [21]. Die Multimetallkomple xe erlauben Untersuchungen von Metall-MetallWechselwirkungen über den Raum bzw.…”

Section: Introductionunclassified

N,N-Disubstituierte Nʹ-Ferrocenoylthioharnstoffe als zweizähnige Komplexliganden für Übergangsmetallionen. Kristallstruktur von Bis-(N,N-diethyl-Nʹ-ferrocenoylthioureato)nickel(II) / N,N-Disubstituted Nʹ-Ferrocenoyl Thioureas as Bidentate Ligands for Transition Metal Ions. Crystal Structure of Bis-(N,N-diethyl-Nʹ-ferrocenoylthioureato)nickel(II)

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Beyer

Richter

1995

Zeitschrift Für Naturforschung B

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N,N-diethyl-Nʹ-ferrocenoyl-thiourea and N-(morpholino-thiocarbonyl)ferrocenecarboxylic amide have been prepared by the reaction of ferrocenoyl chloride with potassium thiocyanate and the respective amine in dry acetone. These bidentate ligands yield neutral heterometalIic complexes with Ni(II), Cu(II), Mn(II) and Co(III). The dark brown air stable crystals of bis-(N,N-diethyl-Nʹ-ferrocenoylthioureato)nickel(II) were characterized by X-ray structure determination. Lattice dimensions: a = 1870.9(1), 6= 1161.5(1), c = 1491.4(1) pm; space group Pca21, Z = 4, R = 0.030 for 5707 observed reflections.

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“…These considerations have led workers to search for alternative labels for antibodies or antigens. Many candidates have been proposed for this role, for example, bacteriophages (Haimovitch et al, 1970), metals (Cais et al, 1977), and free radicals (Leute et al, 1972) but at present the most promising new Received for publication 23 February 1978 labels are enzymes. These can be linked to antibodies or antigens such that the complexes have both immunological and enzymatic activity.…”

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confidence: 99%