Metallo-beta-lactamases of Pseudomonas aeruginosa--a novel mechanism resistance to beta-lactam antibiotics.

Sacha, Paweł; Wieczorek, Piotr; Hauschild, Tomasz; Żórawski, Marcin; Olszańska, Dorota; Tryniszewska, Elżbieta

doi:10.2478/v10042-008-0020-9

Cited by 56 publications

(53 citation statements)

References 51 publications

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“…P. aeruginosa was chosen as a bacterial model to study the antimicrobial properties of the r-Ag NCs. P. aeruginosa has been reported to cause pneumonia, 68 urinary tract infections 68,69 and respiratory system infections in many burn cases 70 and immunesuppressed AIDS patients. 68,71 Recent reports also showed an increased amount of P. aeruginosa resistance towards many conventional antibiotics, such as ampicillin, penicillin and cephalosporin.…”

Section: Superior Antimicrobial Activity Of the Ag Ncsmentioning

confidence: 99%

“…P. aeruginosa has been reported to cause pneumonia, 68 urinary tract infections 68,69 and respiratory system infections in many burn cases 70 and immunesuppressed AIDS patients. 68,71 Recent reports also showed an increased amount of P. aeruginosa resistance towards many conventional antibiotics, such as ampicillin, penicillin and cephalosporin. 24,68 The susceptibility of P. aeruginosa towards r-Ag NCs was first determined by an agar diffusion assay.…”

Section: Superior Antimicrobial Activity Of the Ag Ncsmentioning

confidence: 99%

“…68,71 Recent reports also showed an increased amount of P. aeruginosa resistance towards many conventional antibiotics, such as ampicillin, penicillin and cephalosporin. 24,68 The susceptibility of P. aeruginosa towards r-Ag NCs was first determined by an agar diffusion assay. Our results confirmed that as little as 8 mg of r-Ag NCs can effectively inhibit the growth of P. aeruginosa.…”

Section: Superior Antimicrobial Activity Of the Ag Ncsmentioning

confidence: 99%

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Highly luminescent silver nanoclusters with tunable emissions: cyclic reduction–decomposition synthesis and antimicrobial properties

et al. 2013

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This paper reports a facile aqueous-based synthesis method for highly luminescent Ag nanoclusters (NCs) with tunable emissions. The key strategy was to use a reduction-decomposition-reduction cycle to modify the Ag NC intermediates that were further subjected to size/structure focusing. The as-modified Ag NC intermediates were more robust in water against the subsequent etching by thiol ligands, making a mild size-/structure-focusing environment possible, which produced highly luminescent Ag NCs with a well-defined size and structure. Ag NCs with intense red and green emission were successfully synthesized by this method. These Ag NCs possessed a well-defined size and structure (Ag 16 (SG) 9 and Ag 9 (SG) 6 ), and exhibited excellent stability in the aqueous phase. Our highly luminescent Ag NCs also possessed superior antimicrobial properties against the multidrug-resistant bacteria Pseudomonas aeruginosa, via generating a high concentration of intracellular reactive oxygen species. Keywords: antimicrobial; biomedical applications; luminescence; silver nanoclusters; thiol ligands INTRODUCTION Ultrasmall noble metal nanoclusters (NCs), typically composed of several to a hundred metal atoms, present unique physical and chemical properties between single atoms and large nanocrystals (42 nm). [1][2][3][4] Owing to the strong quantum size confinement in this size range (o 2 nm), NCs possess discrete and size-tunable electronic transitions and display unique molecule-like properties, such as quantized charging and luminescence. [5][6][7][8][9][10][11][12] Strong luminescence is one of the most attractive features of these NCs owing to their ultrafine size, good photostability and low toxicity; 13 some of these properties may not be realized by other luminophores, such as organic dyes (for example, photostability concerns) and semiconductor quantum dots (for example, relatively large size and toxicity concerns). 2,8,9,14,15 Recent studies have shown that luminescent Au and Ag NCs are promising optical probes for bioimaging and biosensing applications. 4,[16][17][18][19][20] This finding has led to the development of various methods for the synthesis of highly luminescent Au and Ag NCs. 2,21 On the other hand, insofar as nanostructured Ag-based materials are concerned, they are finding an increasing usage for antimicrobial applications owing to the unique chemistry of Ag interfacing with microorganisms. [22][23][24] Although there is a large volume of work exploring the potential use of large Ag

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Section: Superior Antimicrobial Activity Of the Ag Ncsmentioning

confidence: 99%

Section: Superior Antimicrobial Activity Of the Ag Ncsmentioning

confidence: 99%

Section: Superior Antimicrobial Activity Of the Ag Ncsmentioning

confidence: 99%

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Highly luminescent silver nanoclusters with tunable emissions: cyclic reduction–decomposition synthesis and antimicrobial properties

et al. 2013

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“…Carbapenem resistance in P. aeruginosa is mostly due to MBLs, including IMP, VIM, SPM, GIM, AIM and DIM enzymes, although other enzymes, including KPC, GES and OXA variants, have been recorded (Sacha et al, 2008;Wang et al, 2010;Juan Nicolau & Oliver, 2010). Multiple mechanisms of resistance often exist in combination (Strateva & Yordanov, 2009;Lister et al, 2009).…”

Section: B-lactamases In Gram-negative Bacteria In Saudi Arabiamentioning

confidence: 99%

The molecular basis of β-lactamase production in Gram-negative bacteria from Saudi Arabia

Yezli

Shibl

Memish

2015

Journal of Medical Microbiology

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Resistance to b-lactams among Gram-negative bacteria is a worldwide issue. Increased prevalence of extended-spectrum b-lactamase (ESBL)-producers and the dissemination of carbapenem-resistance genes are particularly concerning. ESBL-producing strains are common in the Kingdom of Saudi Arabia, particularly among the Enterobacteriaceae, and carbapenem resistance is on the increase, especially among the non-fermenters. b-lactamase production is a major mechanism of resistance to these agents and although b-lactamase-producing strains have been documented in the Kingdom, relatively few reports characterized the molecular basis of this production. Nevertheless, available data suggest that CTX-M (CTX-M-15 in particular) is the predominant ESBL in the Enterobacteriaceae, with SHV also being prevalent in Klebsiella pneumoniae. Carbapenem resistance in the latter is mainly due to OXA-48 and NDM-1. In Pseudomonas aeruginosa, VEB-like enzymes are the most common ESBLs, and VIM is the prevalent metallo-b-lactamase. OXA-10 extended-spectrum enzymes are also frequent. PER and GES ESBLs have been reported in Acinetobacter baumannii, and oxacillinases (OXA-23 in particular) are the dominant carbapanamases in this species. IntroductionInfections due to Gram-negative bacteria are a leading cause of morbidity and mortality worldwide (Giske et al., 2008). Antimicrobial agents are a major therapeutic tool in the treatment of such infections. However, antimicrobial resistance among Gram-negative bacteria, particularly among the Enterobacteriaceae and the non-fermenters, has become one of the most serious public health concerns worldwide (Giske et al., 2008;Livermore, 2012). The global dissemination of resistance has recently received much attention, especially following reports of the international spread of multi-resistant Enterobacteriaceae, particularly strains resistant to cephalosporins due to the production of CTX-M-type extended-spectrum b-lactamases (ESBLs) and strains producing carbapenemases such as KPC and NDM (Nordmann et al., 2011;Johnson & Woodford, 2013). The spread of resistance determinants is facilitated by a number of factors, including presence on genetic mobile elements, antibiotic misuse, poor infection control practices, and increased international travel. In this context Saudi Arabia is particularly relevant as it is annually a host for more than 4 million Muslim pilgrims from over 180 countries worldwide in the Hajj and Umra seasons. In addition, up to 6 million of the Kingdom's population are expatriates, mainly from south and east Asia, where antimicrobial resistance is rife, including to carbapenems (Yong et al., 2009). These factors could potentially make Saudi Arabia a hot spot for the collection of multidrug-resistant strains and the spread of antibiotic resistance around the world.We previously reported on the antimicrobial resistance among the key Gram-negative bacteria in Saudi Arabia and found increased prevalence of b-lactam-resistant strains, including ESBL-producers, and carbapenem resistance (Y...

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“…The spread of this organism is often difficult to control, due to the presence of many intrisinic and acquired mechanisms of antimicrobial resistance [3,4].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of the <i>aacA4</i> gene among multidrug resistant strains of <i>Pseudomonas aeruginosa</i> isolated from bronchial secretions obtained from the Intensive Therapy Unit at University Hospital in Bialystok, Poland

Sacha

Jaworowska

Ojdana

et al. 2012

Folia Histochem Cytobiol.

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Metallo-beta-lactamases of Pseudomonas aeruginosa--a novel mechanism resistance to beta-lactam antibiotics.

Cited by 56 publications

References 51 publications

Highly luminescent silver nanoclusters with tunable emissions: cyclic reduction–decomposition synthesis and antimicrobial properties

Highly luminescent silver nanoclusters with tunable emissions: cyclic reduction–decomposition synthesis and antimicrobial properties

The molecular basis of β-lactamase production in Gram-negative bacteria from Saudi Arabia

Occurrence of the <i>aacA4</i> gene among multidrug resistant strains of <i>Pseudomonas aeruginosa</i> isolated from bronchial secretions obtained from the Intensive Therapy Unit at University Hospital in Bialystok, Poland

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