Metallic trace element body burdens and gene expression analysis of biomarker candidates in Eisenia fetida, using an “exposure/depuration” experimental scheme with field soils

Bernard, Fabien; Brulle, Franck; Douay, Francis; Lemière, Sébastien; Demuynck, Sylvain; Vandenbulcke, Franck

doi:10.1016/j.ecoenv.2010.01.010

Cited by 53 publications

(31 citation statements)

References 58 publications

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“…However, the defense reaction illustrated by the mt gene mRNA expression level was also reflected in the cell parameters' change, especially total cell number, which decreased sharply following Cd, Cu, Zn, and Ni treatment. Cadmium-induced upregulation of the mt gene mRNA expression was likewise shown in E. fetida [26] and Lumbricus rubellus [9,23] or Lumbricus terrestris [46]. Low induction of mt mRNA expression following exposure to other metals could be explained by a lack of ability to induce its biosynthesis.…”

Section: Discussionmentioning

confidence: 87%

Dermal exposure of Eisenia andrei earthworms: Effects of heavy metals on metallothionein and phytochelatin synthase gene expressions in coelomocytes

Homa

Rorat

Kruk

et al. 2015

Enviro Toxic and Chemistry

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Parameters such as total number of coelomocytes, riboflavin content in coelomocytes, expression of genes implied in metal homeostasis, and detoxification mechanisms can be used as biomarkers to assess the impact of metals on annelids. Defense biomarkers (detoxification gene expressions and coelomocyte parameters) were investigated in the ecotoxicologically important species Eisenia andrei following in vivo exposure to 5 different metals (zinc, copper, nickel, lead, and cadmium) at known concentrations. Coelomocyte numbers and riboflavin content were not affected by metallic exposure, but metal-specific gene expression variations were evidenced.

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Section: Discussionmentioning

confidence: 87%

Dermal exposure of Eisenia andrei earthworms: Effects of heavy metals on metallothionein and phytochelatin synthase gene expressions in coelomocytes

Homa

Rorat

Kruk

et al. 2015

Enviro Toxic and Chemistry

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“…It is likely that MT proteins were not yet produced in sufficient quantities to chelate all internalized Cd, and that the remaining internalized Cd accumulated in the insoluble fraction. Previous studies have demonstrated a rapid induction of the mt gene in earthworms exposed to contaminated soils (Brulle et al, 2006(Brulle et al, , 2007Bernard et al, 2010). The induction of mt was mostly assessed in Eisenia fetida and kinetics aspect of gene induction might be very different between the two species.…”

Section: Relationship Between Subcellular Partitioning and Soil Totalmentioning

confidence: 99%

Subcellular partitioning of metals in Aporrectodea caliginosa along a gradient of metal exposure in 31 field-contaminated soils

Beaumelle¹,

Gimbert

Hedde³

et al. 2015

Science of The Total Environment

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“…Quantitative real‐time PCR allows a reliable and fast quantification of gene expression compared with northern‐blot and semi‐quantitative PCR methods (Brulle et al ., ). Differential gene expression by qRT‐PCR has been recently used by a number of researchers to assess the toxicity of different chemicals (Brulle et al ., ; Bernard et al ., ; Rizwan‐ul‐Haq et al ., ). During this study roxarsone caused an increase in the gene expression against the two higher concentrations (150 and 75 mg kg −1 ).…”

Section: Discussionmentioning

confidence: 97%

“…Earthworms have a major role in breaking down different materials in terrestrial environments, as they have strong interaction with the soil. They are being used as toxicity indicators by a number of scientists (Brulle et al, 2006;Sanchez-Hernandez, 2006;Bernard et al, 2010). They have developed different tactics to survive and minimize the damage by employing antioxidant enzymes and specific genes activity to neutralize exotic stress.…”

Section: Discussionmentioning

confidence: 99%

“…The expression levels of target genes were were normalized with the expressions of b-actin (Genbank accession no. DQ286722), whereas ribosomal protein S13 was used as additional control (Bernard et al 2010). Four specimens of earthworms treated with roxarsone and arsanilic acid, were individually dissected as described above, thus making four repeats against each chemical at a specific time interval (Rizwan-ul-Haq et al, 2011).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

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Evaluation of glutathione s‐transferase as toxicity indicator for roxarsone and arsanilic acid in Eisenia fetida

Rizwan-ul-Haq

Zeng

Sun

et al. 2012

J of Applied Toxicology

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Different compounds can induce stress response by targeting specific genes. Studies related to elucidating the detoxification and adaptive responses of proteins like glutathione-s-transferase (GST) can be helpful in better understanding toxicity. Roxarsone and arsanilic acid, which have been exhaustively used as animal and poultry feed additives, pose a threat to the environment and human health. GST enzyme bioassay revealed fluctuations in response to different concentrations of roxarsone and arsanilic acid at different time intervals. The highest GST enzyme activity (40.51%) was observed on day 15 of treatment with roxarsone. On the other hand, arsanilic acid caused the maximum enzyme activity (52.11%) on day 10 of treatment. During this study, the full-length gene sequence of GST, having the size 984 bp (Genbankno. HQ693699), was achieved from Eisenia fetida and established as a biomarker to assess the toxicity of roxarsone and arsanilic acid. The deduced protein has a computed molecular mass of 23.56 kDa and a predicted isoelectric point of 9.92. Quantitative real-time PCR revealed significant differential gene expression in response to roxarsone and arsanilic acid treatment as compared with control treatment. Roxarsone caused the highest gene expression of 7.0-fold increase over control on day 15 of treatment, whereas arsanilic acid resulted in the highest gene expression reaching to 14.56-fold as compared with control. This study is helpful in understanding the role of GST as a potential biomarker for chemicals like roxarsone and arsanilic acid, which can pollute the food chain.

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Metallic trace element body burdens and gene expression analysis of biomarker candidates in Eisenia fetida, using an “exposure/depuration” experimental scheme with field soils

Cited by 53 publications

References 58 publications

Dermal exposure of Eisenia andrei earthworms: Effects of heavy metals on metallothionein and phytochelatin synthase gene expressions in coelomocytes

Dermal exposure of Eisenia andrei earthworms: Effects of heavy metals on metallothionein and phytochelatin synthase gene expressions in coelomocytes

Subcellular partitioning of metals in Aporrectodea caliginosa along a gradient of metal exposure in 31 field-contaminated soils

Evaluation of glutathione s‐transferase as toxicity indicator for roxarsone and arsanilic acid in Eisenia fetida

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