Metallic crown-induced occlusal trauma as a protocol to evaluate inflammatory response in temporomandibular joint and periodontal tissues of rats

Abdalla, Henrique Ballassini; Clemente-Napimoga, Juliana Trindade; Bonfante, Ricardo; Hashizume, Caio Augusto; Zanelli, Wilian Segatto; Macedo, Cristina Gomes de; Napimoga, Marcelo Henrique; Silva, Wilkens Aurélio Buarque e; Silva, Frederico Andrade e

doi:10.1007/s00784-018-2639-z

Cited by 14 publications

(25 citation statements)

References 34 publications

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“…We demonstrated that occlusal trauma increases the protein levels of TNF‐α and IL‐6 in gingival tissues. In a previous study, we reported that TNF‐α and IL‐1β were significantly higher when compared to the sham group 16 . These results confirmed the installed inflammatory reaction resulting from traumatic injury.…”

Section: Discussionsupporting

confidence: 75%

“…After 28 days of metal crown cementation, 16 the animals were euthanized. Samples from the gingival tissue surrounding the lower first molar were collected and homogenized individually in an appropriate buffer containing protease inhibitors (Ripa Lysis Buffer, Santa Cruz, Biotechnology, Dallas, TX) followed by centrifugation at 10,000 rpm for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, n = 6.25. Considering the previous study 16 and in order to minimize the number of animals used, the sample size was 6 per group. Data were first examined for normality using the Kolmogorov‐Smirnov test.…”

Section: Methodsmentioning

confidence: 99%

“…Also, tooth extraction is an extremely invasive model with more cons than pros, such as the risk of infection and bleeding during the procedure, not to mention that loss of bone under such conditions is not induced by occlusal trauma. To avoid the aforementioned limitations, a new model of ETO using custom‐made metal crowns with occlusal anatomy has recently been introduced 16 …”

Section: Introductionmentioning

confidence: 99%

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Occlusion Heightened by Metal Crown Cementation is Aggressive for Periodontal Tissues

Abdalla

Clemente‐Napimoga

Trindade‐da‐Silva

et al. 2020

Journal of Prosthodontics

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Purpose To investigate the effect of experimental traumatic occlusion (ETO) induced by metal crowns on alveolar bone loss. Materials and Methods Metal crowns were custom‐made for the lower first molars with occlusal discrepancy of 0.4 and 0.7 mm from the maximum intercuspation. Thirty‐six animals were randomly divided into three groups (n = 12 animals per group): 0.4‐mm hyperocclusion group, 0.7‐mm hyperocclusion group and the sham group (no metal crown). Twenty‐eight days after crown cementation, the animals were euthanized and gingival tissue was collected to assess cytokine levels of IL‐17, IL‐6, and TNF‐α using enzyme‐linked immunosorbent assay (ELISA). Mandibles were stained with 1% methylene blue and alveolar bone levels were quantified. Western blotting was used to quantify the expression of receptor activator of nuclear factor κ B (RANK), and its ligand (RANKL), secreted osteoclastogenic factor of activated T cells (SOFAT) and TNF‐α‐converting enzyme (TACE). Also, mandibles were histologically processed and stained with hematoxylin and eosin, from which the presence of osteoclast‐like cells, multinucleated cells containing ≥3 nuclei was counted at 100× magnification. The data were analyzed using one‐way ANOVA and Tukey tests. Results Experimental occlusal trauma for 28 consecutive days significantly increased alveolar bone loss and multinucleated cell counts (p < 0.05). RANK, RANKL, SOFAT, TACE, IL‐6, and TNF‐α were significantly higher in gingival tissues of ETO groups (p < 0.05). IL‐17 titers were unchanged among the groups (p > 0.05). Conclusion Experimental traumatic occlusion activates and sustains bone resorption pathways in the periodontium inducing alveolar bone resorption. As the intensity of occlusal trauma increased, alternative osteoclastic pathways were activated, such as TACE and SOFAT.

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Section: Discussionsupporting

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occlusion Heightened by Metal Crown Cementation is Aggressive for Periodontal Tissues

Abdalla

Clemente‐Napimoga

Trindade‐da‐Silva

et al. 2020

Journal of Prosthodontics

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“…In vitro mechanical stress induced the mRNA expression levels of TNF‐α in PDL cells . It has been reported that traumatic occlusion induced the release of TNF‐α in rat model . In this study, glyburide might reduce bone resorption by suppressing the expression of TNF‐α and IL‐6.…”

Section: Discussionmentioning

confidence: 67%

Glyburide inhibits the bone resorption induced by traumatic occlusion in rats

Arita

Yoshinaga

Kaneko

et al. 2020

J of Periodontal Research

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Objective To examine whether glyburide inhibits bone destruction caused by traumatic occlusion in a rat occlusal trauma model. Background Excessive mechanical stress, such as traumatic occlusion, induces expression of IL‐1β and may be involved in bone resorption. NLRP3 inflammasomes have been linked to IL‐1β expression, but it is currently unclear whether glyburide, the inhibiter of NLRP3 inflammasome, suppresses occlusal trauma in rats. Methods Male SD rats aged 7 weeks were used. In the trauma group, the occlusal surface of the maxillary first right molar was raised by attaching a metal wire to apply occlusal trauma to the mandibular first right molar. In the trauma + glyburide group, the NLRP3 inhibitor glyburide was administered orally every 24 hours from 1 day before induction of occlusal trauma. Rats were euthanized after 5 or 10 days, and the maxillary first molars were harvested with the adjacent tissues for histopathological investigation. Immunohistochemical expression of IL‐1β, NLRP3, and RANKL was also assessed. Results On day 5, bone resorption was significantly greater in the trauma group compared with the control group or the trauma + glyburide group, and there were significantly higher numbers of osteoclasts and cells positive for IL‐1β, NLRP3, and RANKL in the trauma group. Conclusion In this study, glyburide inhibits bone resorption by traumatic occlusion in rats. It suggests that the NLRP3/IL‐1β pathway might be associated with bone resorption induced by traumatic occlusion.

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Fast and accurate protocol for histology and immunohistochemistry reactions in temporomandibular joint of rats

Basting

Napimoga

Lima

et al. 2021

Archives of Oral Biology

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Metallic crown-induced occlusal trauma as a protocol to evaluate inflammatory response in temporomandibular joint and periodontal tissues of rats

Abstract: The standardization of an experimental occlusal interference model will allow us to understand the deleterious effect and mechanisms that affect the orofacial tissues.

Cited by 14 publications

References 34 publications

Occlusion Heightened by Metal Crown Cementation is Aggressive for Periodontal Tissues

Occlusion Heightened by Metal Crown Cementation is Aggressive for Periodontal Tissues

Glyburide inhibits the bone resorption induced by traumatic occlusion in rats

Fast and accurate protocol for histology and immunohistochemistry reactions in temporomandibular joint of rats

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