Metal Transport in the Rhizobium-Legume Symbiosis

Guerrero, Manuel Gonzalez; Sanz, Laura Rubio; Haas, Benjamin Rodriguez; Albareda, Marta; Cerón, M. Menéndez; Lopez, Maria Belen Brito; Palacios, José Luis Calvo

doi:10.1201/b15251-8

Cited by 2 publications

(2 citation statements)

References 138 publications

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“…Analyzing more deeply the 842 transcripts (Table S7) that were both significantly and highly up‐regulated in nodules compared to other organs (adjusted P ‐value < 0.05, fold change > 10) revealed candidates for components of nodule development (including signaling, regulatory and organogenesis components) as well as nodule function (including bacteroid energy source, oxygen and mineral nutrition, stress management, nitrogen compound metabolism and export). Signaling and transport were the most highly represented classes of functions, as reported in other species (González‐Guerrero et al ., ; Udvardi and Poole, ; O'Rourke et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Full‐length de novo assembly of RNA‐seq data in pea (Pisum sativum L.) provides a gene expression atlas and gives insights into root nodulation in this species

et al. 2015

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Summary Next‐generation sequencing technologies allow an almost exhaustive survey of the transcriptome, even in species with no available genome sequence. To produce a Unigene set representing most of the expressed genes of pea, 20 cDNA libraries produced from various plant tissues harvested at various developmental stages from plants grown under contrasting nitrogen conditions were sequenced. Around one billion reads and 100 Gb of sequence were de novo assembled. Following several steps of redundancy reduction, 46 099 contigs with N50 length of 1667 nt were identified. These constitute the ‘Caméor’ Unigene set. The high depth of sequencing allowed identification of rare transcripts and detected expression for approximately 80% of contigs in each library. The Unigene set is now available online (http://bios.dijon.inra.fr/FATAL/cgi/pscam.cgi), allowing (i) searches for pea orthologs of candidate genes based on gene sequences from other species, or based on annotation, (ii) determination of transcript expression patterns using various metrics, (iii) identification of uncharacterized genes with interesting patterns of expression, and (iv) comparison of gene ontology pathways between tissues. This resource has allowed identification of the pea orthologs of major nodulation genes characterized in recent years in model species, as a major step towards deciphering unresolved pea nodulation phenotypes. In addition to a remarkable conservation of the early transcriptome nodulation apparatus between pea and Medicago truncatula, some specific features were highlighted. The resource provides a reference for the pea exome, and will facilitate transcriptome and proteome approaches as well as SNP discovery in pea.

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Section: Discussionmentioning

confidence: 99%

Full‐length de novo assembly of RNA‐seq data in pea (Pisum sativum L.) provides a gene expression atlas and gives insights into root nodulation in this species

et al. 2015

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“…Due to the difficulty in working with bacteroids, metal transport and accumulation during symbiotic conditions is still poorly understood. [35] Even more, it is really difficult to locate (outer membrane, periplasm or inner membrane) Cu accumulation even in free bacterial cells. [36] It can be concluded that inoculation of M. truncatula with the genetically modified strain expressing copAB ameliorated plant growth, enhanced plant nodulation and nitrogen fixation and increased Cu phytostabilization at moderate Cu concentrations.…”

Section: Discussionmentioning

confidence: 99%

Improving legume nodulation and Cu rhizostabilization using a genetically modified rhizobia

Delgadillo

Lafuente

Doukkali

et al. 2014

Environmental Technology

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The rhizobia-legume interaction has been proposed as an interesting and appropriate tool for rhizostabilization of soils contaminated with heavy metals. One of the main requirements to use this symbiosis is the availability of tolerant and symbiotically effective rhizobia. The aim of this work was to improve the symbiotic properties of the arsenic-resistant wild-type strain Ensifer medicae MA11 in Cu-contaminated substrates. The copAB genes from a Cu-resistant Pseudomonas fluorescens strain were expressed in E. medicae MA11 under the control of the nifH promoter. The resulting strain E. medicae MA11-copAB was able to alleviate the toxic effect of Cu in Medicago truncatula. At 300 µM Cu, root and shoot dry matter production, nitrogen content, number of nodules and photosynthetic rate were significantly reduced in plants inoculated with the wild-type strain. However, these parameters were not altered in plants inoculated with the genetically modified strain. Moreover, nodules elicited by this strain were able to accumulate twofold the Cu measured in nodules formed by the wild-type strain. In addition, the engineered E. medicae strain increased Cu accumulation in roots and decreased the content in shoots. Thus, E. medicae MA11-copAB increased the capacity of M. truncatula to rhizostabilize Cu, decreasing the translocation factor and avoiding metal entry into the food chain. The plasmid containing the nifH promoter-copAB construct could be a useful biotool for Cu rhizostabilization using legumes, since it can be transferred to different rhizobia microsymbionts of authoctonous legumes growing in Cu-contaminated soils.

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Metal Transport in the Rhizobium-Legume Symbiosis

Cited by 2 publications

References 138 publications

Full‐length de novo assembly of RNA‐seq data in pea (Pisum sativum L.) provides a gene expression atlas and gives insights into root nodulation in this species

Full‐length de novo assembly of RNA‐seq data in pea (Pisum sativum L.) provides a gene expression atlas and gives insights into root nodulation in this species

Improving legume nodulation and Cu rhizostabilization using a genetically modified rhizobia

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