Metal transport in<i>Medicago truncatula</i>nodule rhizobia‐infected cells

Abreu, Isidro; Escudero, Viviana; Montiel, Jesús; Castro-Rodriguez, Rosario; González‐Guerrero, Manuel

doi:10.1002/9781119409144.ch81

Cited by 1 publication

(2 citation statements)

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“…Our results suggest that the Zn deficiency response regulated by F-bZIP transcription factors is conserved in the legume model M. truncatula . In legumes species, an important component of the plant Zn homeostasis is the transport of Zn to the nodule-rhizobia infected cells where an adequate delivery to metalloproteins is required for proper nitrogen fixation ( Abreu et al, 2020 ). It is conceivable that the Zn deficiency response includes the regulation of Zn homeostasis in symbiosomes in M. truncatula.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have particularly targeted the transport and delivery of Zn and other metal micronutrients to the nodule-rhizobia infected cells ( López-Millán et al, 2004 ; Avenhaus et al, 2016 ; Abreu et al, 2017 ; Escudero et al, 2020 ). In legume species, this is an important component of the plant metal micronutrient homeostasis, with a significant allocation to the symbiosome, where an adequate metal delivery and availability is essential for metalloprotein requirements and a proper symbiotic nitrogen fixation ( Abreu et al, 2020 ). Here, considering the evolutionary conservation of the F-bZIP transcription factors ( Castro et al, 2017 ), we investigated the Zn deficiency response in the legume model M. truncatula.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of the Zinc Deficiency Response in the Legume Model Medicago truncatula

et al. 2022

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The zinc deficiency response in Arabidopsis thaliana is regulated by F-group basic region leucine-zipper (F-bZIP) transcription factors, and there is evidence of evolutionary conservation of this regulatory network in land plants. Fundamental knowledge on the zinc homeostasis regulation in crop species will contribute to improving their zinc nutritional value. Legumes are protein-rich crops, used worldwide as part of traditional diets and as animal forage, being therefore a good target for micronutrient biofortification. Here, we identified F-bZIP transcription factors in representative legume species and functionally characterized the two F-bZIPs from Medicago truncatula. Results indicate that MtFbZIP1 is the functional homolog of A. thaliana bZIP19 and bZIP23, while MtFbZIP2 does not play a role in the zinc deficiency response. Additionally, analysis of M. truncatula genes from the Zrt/Irt-like protein (ZIP) family of zinc transporters or encoding nicotianamine synthase enzymes that produce the zinc ligand nicotianamine, support the conservation of the F-bZIP-regulated zinc deficiency response in M. truncatula. Phylogenetic analysis of F-bZIP homologs enriched in legume species reinforces the branching into two groups, with MtFbZIP1 and MtFbZIP2 mapping in Groups 1 and 2, respectively. This phylogeny combined with the functional characterization of MtFbZIPs supports the suggested conservation of the zinc deficiency response associated with Group 1 F-bZIPs, and the more variable evolutionary paths associated with Group 2. Overall, we provide novel insight on the mechanisms of response to zinc deficiency in M. truncatula, which contributes to developing strategies for improving zinc content in legume crops.

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Section: Discussionmentioning

confidence: 99%