Leaf Associated Microbial Activities in a Stream Affected byAcid Mine Drainage key words: leaf litter breakdown, acid mine drainage, respiration rate, ochre deposition, iron plaques
AbstractMicrobial activity was assessed on birch leaves and plastic strips during 140 days of exposure at three sites in an acidic stream of the Lusatian post-mining landscape, Germany. The sites differed in their degrees of ochre deposition and acidification. The aim of the study was (1) to follow the microbial activities during leaf colonization, (2) to compare the effect of different environmental conditions on leaf associated microbial activities, and (3) to test the microbial availability of leaf litter in acidic mining waters. The activity peaked after 49 days and subsequently decreased gradually at all sites. A formation of iron plaques on leaf surfaces influenced associated microbial activity. It seemed that these plaques inhibit the microbial availability of leaf litter and serve as a microbial habitat by itself.
IntroductionAcid mine drainage is a persistent effect of human resource usage and affect many aquatic ecosystems throughout the world (NOIKE et al., 1983;MCKNIGHT and FEDER, 1984;SCHULTZE and GELLER, 1996). The acid drainage is generated by the oxidative weathering of sulphidecontaining minerals, which often lies in or around coal seams. Streams affected by acid mine drainage typically have low pH values, high concentrations of sulphate and iron, and abundant deposition of precipitated metal oxides, predominantly ochre (iron oxyhydroxide), that are covering the streambed (ENDER and LESSMANN, 2000). The complex interaction of all these stressors can lead to severe alterations of the biological structure and ecosystem processes. Consequently, the examination of ecosystem processes, like leaf litter breakdown, is one proposed measure to test the integrity of such affected streams (GESSNER and CHAU- VET, 2002). Leaf litter breakdown is a well-documented process, in which the interplay of abiotic and biotic subprocesses leads to a disintegration of leaves (WEBSTER and BENFIELD, 1986;GESSNER et al., 1999). A number of recent investigations revealed that the leaf litter breakdown process in acidic mining streams is slow and primarily microbial mediated, due to the absence of macroinvertebrate "shredders" (SIEFERT and MUTZ, 2000;NIYOGI et al., 2001 and. The authors assume that, like in circumneutral aquatic environments, microorganisms exploit leaf material through enzymatic cleavage of plant polymers in acidic mining waters. If this is true, one can predict higher microbial activities on natural leaf litter than on an artificial material that can only serve as a holdfast for microorganisms. Leaf associated respiration rates are a suitable measure to assess microbial activity and were already applied by GROOM and HILDREW (1989) and NIYOGI et al., (2001) and (2002) in acidic streams. These studies, however, gave no information about the temporal dynamics of leaf associated microbial activity.Internat. Rev. Hydrobiol.