Metal transfer to sediments, invertebrates and fish following waterborne exposure to silver nitrate or silver sulfide nanoparticles in an indoor stream mesocosm

High throughput laser ablation ICP-MS bioimaging of silver distribution in animal organisms and plant tissue after exposure to silver sulfide nanoparticles

“…These findings were also confirmed in the study by Clark et al 34 where total metal concentrations in the organs of the fish reflected the lower bioavailability of Ag 2 S compared to AgNO 3 . Clear differences in Ag biokinetics were also shown in the study by Talaber et al 4 where Ag bioaccumulation after exposure of two soil invertebrate species to pristine Ag NPs, ionic Ag and Ag 2 S NPs (same as those used in our study) depended on the Ag form, soil type and test organism.…”

“…For the aquatic organisms, an indoor modular mesocosm system was used to simulate a stream environment. For a detailed description of the experimental design, see Clark et al 34 Briefly, the system was composed of 36 artificial rivers made of glass arranged in triplicate, and four sets of triplicate were assigned to controls, Ag 2 S NPs, and AgNO 3 . The bottom of each stream was covered by a layer of sediment mixed with ground alder leaves, and streams were filled with an enriched artificial pond water (APW) medium.…”

Instance maps as an organising concept for complex experimental workflows as demonstrated for (nano)material safety research

“…Ecotoxicity exposures conducted in soil and mesocosm experiments are often complex with multiple parameters and endpoints (e.g., [37][38][39][40]). The diversity of data types required to monitor soil, porewater, nanomaterials and organisms requires many sample collections and analyses, as well as ensuring collection of pre-experiment data and metadata.…”

Toxicokinetics and bioaccumulation of silver sulfide nanoparticles in benthic invertebrates in an indoor stream mesocosm

“…In this paper, the sampling procedure and toxicokinetic data for G. tigrina, P. acuta and C. riparius are presented. Details for the other species are reported by Clark et al (2022), which also reports the fate and concentrations of Ag in water, sediments and biota of this mesocosm experiment. Therefore, some data derived from Clark et al (2022) is also presented in this paper as it is crucial for understanding Ag exposure, uptake and toxicokinetics in the test species.…”

“…Subsamples of around 250 mg dry sediment were digested in covered 50 mL glass beakers using 10 mL neat nitric acid, following Chen and Ma (2001). A more detailed description of the sampling procedure and analysis of biota, water and sediment samples can be found in Clark et al (2022).…”