Metal resistance in Candida biofilms

Harrison, Joe J.; Rabiei, Maryam; Turner, Raymond J.; Sproule, Kimberley M.

doi:10.1111/j.1574-6941.2005.00045.x

Cited by 90 publications

(101 citation statements)

References 47 publications

(80 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5B), which were not evident in the former. These findings are in agreement with other studies reporting that the sessile growth mode might lead to a lower susceptibility to metal ions than the free-living mode (37,46). Thus, in addition to fulfilling the structural criteria suggested by Harding et al (2), P. ostreatus biofilms exhibited a commonly observed hallmark among microbial biofilms, namely, an increased resistance to toxicants compared to the respective free-floating state (2,11).…”

Section: Discussionsupporting

confidence: 82%

See 1 more Smart Citation

Characterization of Pleurotus ostreatus Biofilms by Using the Calgary Biofilm Device

Pesciaroli

Petruccioli

Fedi

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

The adequacy of the Calgary biofilm device, often referred to as the MBEC system, as a high-throughput approach to the production and subsequent characterization of Pleurotus ostreatus biofilms was assessed. The hydroxyapatite-coating of pegs was necessary to enable biofilm attachment, and the standardization of vegetative inocula ensured a uniform distribution of P. ostreatus biofilms, which is necessary for high-throughput evaluations of several antimicrobials and exposure conditions. Scanning electron microscopy showed surface-associated growth, the occurrence of a complex aggregated growth organized in multilayers or hyphal bundles, and the encasement of hyphae within an extracellular matrix (ECM), the extent of which increased with time. Chemical analyses showed that biofilms differed from free-floating cultures for their higher contents of total sugars (TS) and ECM, with the latter being mainly composed of TS and, to a lesser extent, protein. Confocal laser scanning microscopy analysis of 4-day-old biofilms showed the presence of interspersed interstitial voids and water channels in the mycelial network, the density and compactness of which increased after a 7-day incubation, with the novel occurrence of ECM aggregates with an ␣-glucan moiety. In 4-and 7-day-old biofilms, tolerance to cadmium was increased by factors of 3.2 and 11.1, respectively, compared to coeval free-floating counterparts.

show abstract

Section: Discussionsupporting

confidence: 82%

“…In a comparative study, HA best supported the biofilm growth of both Candida glabrata and Candida albicans (36). In addition, the inability of polystyrene pegs to gain satisfactory biofilm amounts was observed with the fungus Candida tropicalis, and coating with L-lysine was needed for this purpose (37).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Pleurotus ostreatus Biofilms by Using the Calgary Biofilm Device

Pesciaroli

Petruccioli

Fedi

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…The inability to clear infection may also be related to biofilm formation (10), which we previously showed to occur in E. coli-induced chronic prostatitis (5). In vitro biofilm formation has been compared previously using the Calgary biofilm device (2,9,21), and in this study no difference in biofilm (Fig. 6) or planktonic growth (data not shown) was seen between the WT and the ZapA Ϫ mutant.…”

Section: Discussionmentioning

confidence: 88%

ZapA, a Virulence Factor in a Rat Model of Proteus mirabilis -Induced Acute and Chronic Prostatitis

2008

Self Cite

View full text Add to dashboard Cite

Our knowledge of pathogenesis has benefited from a better understanding of the roles of specific virulence factors in disease. To determine the role of the virulence factor ZapA, a 54-kDa metalloproteinase of Proteus mirabilis, in prostatitis, rats were infected with either wild-type (WT) P. mirabilis or its isogenic ZapA ؊ mutant KW360. The WT produced both acute and chronic prostatitis showing the typical histological progressions that are the hallmarks of these diseases. Infection with the ZapA ؊ mutant, however, resulted in reduced levels of acute prostatitis, as determined from lower levels of tissue damage, bacterial colonization, and inflammation. Further, the ZapA ؊ mutant failed to establish a chronic infection, in that bacteria were cleared from the prostate, inflammation was resolved, and tissue was seen to be healing. Clearance from the prostate was not the result of a reduced capacity of the ZapA ؊ mutant to form biofilms in vitro. These finding clearly define ZapA as an important virulence factor in both acute and chronic bacterial prostatitis.Proteus mirabilis can be an important infectious agent of the urinary tract that can lead to pyelonephritis or renal calculus, especially in those who are catheterized or who have anomalies in their urinary tract (16,24,31). ZapA, which is an extracellular metalloprotease, is one of many well-characterized virulence factors important in urinary tract infections caused by P. mirabilis (3,16,26,27). ZapA is a broad-spectrum protease which degrades a number of substrates, including immunoglobulin A (IgA) and defensins of the host's immune system (26, 27). Swarmer cell formation, which is important in the pathogenesis of this organism, results in the upregulation of many P. mirabilis virulence factors, including ZapA (1,27). This appears to be influenced by the ability of P. mirabilis to sense surfaces (4), and, not surprisingly, swarmer cell formation is also seen in biofilm formation in artificial urine (12), which may explain the link of pathogenesis to catheterization and anomalies in the urinary tract, where biofilm formation will be encouraged.Prostatitis is the leading cause for men under 50 years of age to seek treatment from a urologist. The etiology of this disease remains confusing, as bacteria are isolated from only 5 to 10% of clinical cases; however, this is believed to represent a gross underestimation of infection (18,20). Bacterial prostatitis occurs both as an acute febrile disease that as often responsive to antibiotic treatment and as a chronic infection that is too often nonresponsive to antibiotic treatment and is believed to involve bacterial biofilm formation. Bacterial prostatitis is believed to be the leading cause of recurrent urinary tract infections in men (18,20). Our lab has established models of bacterial and nonbacterial prostatitis in rats (7,13,19) and has demonstrated biofilm formation at the mucosal surface of the prostatic acini in this model (5).It has been well established that distinct virulence factors are important in bo...

show abstract

“…The Tryptic Soy Broth (TSB, MossHeMoss) medium was used as a nutritious medium for culturing of planktonic cells and biofilms of bacteria and yeasts (HARRISON et al, 2006). The suspension of microorganisms has been designed to match the McFarland 1.0.…”

Section: Organisms and Growth Conditionsmentioning

confidence: 99%

“…Fluorescence microscopy was used to evaluate the impact of the test substances on biofilms according to the method of HARRISON et al (2006). Biofilms were treated with tested substances as described above.…”

Section: Fluorescence Microscopy Analysismentioning

confidence: 99%

Difference in influence of commercial industrial paints on microbial biofilms and planktonic cells

Grujić¹,

Radevic²,

Radojević³

et al. 2017

Kragujevac J Sci

View full text Add to dashboard Cite

ABSTRACT. This study compares the effect of commercial industrial paints on the Escherichia coli PMFKG-F2, Proteus mirabilis PMFKG-F4 and Saccharomyces cerevisiae PMFKG-F6 planktonic cells and biofilms. A MBEC TM -HTP assay and standard 96 microtiter plate assay were used to test the levels of resistance of planktonic cells and biofilms. The minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) of the tested substances, which affects planktonic cells and biofilms, were determined and the results were confirmed by fluorescence microscopy.Results obtained for planktonic cells were compared between them and with the results obtained for biofilms. Noticeable difference in the resistance between the biofilms and the planktonic cells on paints, was observed. The E. coli PMFKG-F2 planktonic cells showed the highest resistance in the presence of the tested substance 2 (MICp 2.5 µl/ml), while the P. mirabilis PMFKG-F4 planktonic cells showed the highest resistance in the presence of the tested substance 2 (MICp 5 µl/ml). The S. cerevisiae PMFKG-F6 planktonic cells showed the same level of resistance in the presence of the tested substances 1, 2 and 5 (MICp 0.62 µl/ml). The E. coli PMFKG-F2, P. mirabilis PMFKG-F4 and S. cerevisiae PMFKG-6 biofilms showed the highest resistance in the presence of the tested substance 5 (MICb 125 µl/ml, MICb 125 µl/ml and MICb 62.5 µl/ml).The obtained results suggest that the biofilm may have a potential to be used in bioremediation of wastewater contaminated with industrial paints.

show abstract

Metal resistance in Candida biofilms

Cited by 90 publications

References 47 publications

Characterization of Pleurotus ostreatus Biofilms by Using the Calgary Biofilm Device

Characterization of Pleurotus ostreatus Biofilms by Using the Calgary Biofilm Device

ZapA, a Virulence Factor in a Rat Model of Proteus mirabilis -Induced Acute and Chronic Prostatitis

Difference in influence of commercial industrial paints on microbial biofilms and planktonic cells

Contact Info

Product

Resources

About