Metal Resistance and Accumulation in Cyanobacteria

Fiore, Marli Fátima; Moon, David Henry; Trevors, J. T.

doi:10.1007/978-3-662-10863-5_7

Cited by 7 publications

(4 citation statements)

References 73 publications

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“…To our knowledge, no information regarding the tolerance of C. regularis to metals is found in the literature [46]. Cha‐maesiphon regularis occurs in cool mountain streams [45].…”

Section: Discussionmentioning

confidence: 99%

Metal toxicity inferred from algal population density, heterotrophic substrate use, and fatty acid profile in a small stream

Genter¹,

Lehman

2000

Enviro Toxic and Chemistry

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The purpose of this study was to examine relations between metal concentrations in periphyton and the abundance of algal species, heterotrophic use of 95 carbon sources, and phospholipid fatty acids (PLFA) of the periphyton in a small stream spanning a mine in Lemhi County, Idaho, USA. Two upstream, two mine, and two downstream sites were examined. Elevated concentrations of As and Cu at the mine sites were associated with communities that were depleted of diatoms and filamentous blue‐green algae and characterized by a low‐diversity community dominated by a single blue‐green alga and patchy populations of the diatom Achnanthidium minutissimum and a filamentous green alga. Carbon source use and PLFA profiles provided a rapid assessment of stream conditions that were consistent with algal taxonomy and with our hypotheses constructed from previous reports on periphyton responses to metal stress.

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“…To our knowledge, no information regarding the tolerance of C. regularis to metals is found in the literature [46]. Cha‐maesiphon regularis occurs in cool mountain streams [45].…”

Section: Discussionmentioning

confidence: 99%

Metal toxicity inferred from algal population density, heterotrophic substrate use, and fatty acid profile in a small stream

Genter¹,

Lehman

2000

Enviro Toxic and Chemistry

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“…To our knowledge, no information regarding the tolerance of C. regularis to metals is found in the literature [46]. Chamaesiphon regularis occurs in cool mountain streams [45].…”

Section: Algaementioning

confidence: 99%

Metal Toxicity Inferred From Algal Population Density, Heterotrophic Substrate Use, and Fatty Acid Profile in a Small Stream

Genter¹,

Lehman

2000

Environ Toxicol Chem

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Abstract-The purpose of this study was to examine relations between metal concentrations in periphyton and the abundance of algal species, heterotrophic use of 95 carbon sources, and phospholipid fatty acids (PLFA) of the periphyton in a small stream spanning a mine in Lemhi County, Idaho, USA. Two upstream, two mine, and two downstream sites were examined. Elevated concentrations of As and Cu at the mine sites were associated with communities that were depleted of diatoms and filamentous blue-green algae and characterized by a low-diversity community dominated by a single blue-green alga and patchy populations of the diatom Achnanthidium minutissimum and a filamentous green alga. Carbon source use and PLFA profiles provided a rapid assessment of stream conditions that were consistent with algal taxonomy and with our hypotheses constructed from previous reports on periphyton responses to metal stress.

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“…Ancient cyanobacteria as photosynthetic prokaryotes are globally widespread, found from seawater and freshwater systems to soils and even deserts . Cyanobacteria often grow in heavy metal(loid)-contaminated habitats due to their ability to resist metal toxicity . Previous studies have indicated that cyanobacteria have multiple As biotransformation pathways and, in particular, make great contributions to As methylation .…”

Section: Introductionmentioning

confidence: 99%

“…32 Cyanobacteria often grow in heavy metal(loid)-contaminated habitats due to their ability to resist metal toxicity. 33 Previous studies have indicated that cyanobacteria have multiple As biotransformation pathways and, in particular, make great contributions to As methylation. 14 However, it is still unclear whether cyanobacteria are associated with the reverse reaction, As demethylation, not to mention the molecular mechanism.…”

Section: ■ Introductionmentioning

confidence: 99%

Arsenic Demethylation by a C·As Lyase in Cyanobacterium Nostoc sp. PCC 7120

Yan

Xue

et al. 2015

Environ. Sci. Technol.

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Arsenic, a ubiquitous toxic substance, exists mainly as inorganic forms in the environment. It is perceived that organoarsenicals can be demethylated and degraded into inorganic arsenic by microorganisms. Few studies have focused on the mechanism of arsenic demethylation in bacteria. Here, we investigated arsenic demethylation in a typical freshwater cyanobacterium Nostoc sp. PCC 7120. This bacterium was able to demethylate monomethylarsenite [MAs(III)] rapidly to arsenite [As(III)] and also had the ability to demethylate monomethylarsenate [MAs(V)] to As(III). The NsarsI encoding a C·As lyase responsible for MAs(III) demethylation was cloned from Nostoc sp. PCC 7120 and heterologously expressed in an As-hypersensitive strain Escherichia coli AW3110 (ΔarsRBC). Expression of NsarsI was shown to confer MAs(III) resistance through arsenic demethylation. The purified NsArsI was further identified and functionally characterized in vitro. NsArsI existed mainly as the trimeric state, and the kinetic data were well-fit to the Hill equation with K0.5 = 7.55 ± 0.33 μM for MAs(III), Vmax = 0.79 ± 0.02 μM min(-1), and h = 2.7. Both of the NsArsI truncated derivatives lacking the C-terminal 10 residues (ArsI10) or 23 residues (ArsI23) had a reduced ability of MAs(III) demethylation. These results provide new insights for understanding the important role of cyanobacteria in arsenic biogeochemical cycling in the environment.

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Metal Resistance and Accumulation in Cyanobacteria

Cited by 7 publications

References 73 publications

Metal toxicity inferred from algal population density, heterotrophic substrate use, and fatty acid profile in a small stream

Metal toxicity inferred from algal population density, heterotrophic substrate use, and fatty acid profile in a small stream

Metal Toxicity Inferred From Algal Population Density, Heterotrophic Substrate Use, and Fatty Acid Profile in a Small Stream

Arsenic Demethylation by a C·As Lyase in Cyanobacterium Nostoc sp. PCC 7120

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