Metal phthalocyanine as photosensitizer for photodynamic therapy (PDT)

Huang, Jinling; Chen, Naisheng; Huang, Jian‐Dong; Liu, Er-Sheng; Xue, Jinping; Suling, Yang; Huang, Ziqiang; Sun, Juan

doi:10.1007/bf02879529

Cited by 44 publications

(16 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The wavelength of suitably excited light for ZnPcS 2 P 2 is 670 nm. 22 In ZnPcS 2 P 2 , the number ratio of the hydrophilic group (S group) and lipophilic group (P group) is 2:2. These two groups are cis arrangement in ZnPcS 2 P 2 molecule.…”

Section: Methodsmentioning

confidence: 99%

“…A novel amphipathic photosensitizer, di-sulfo-di-phthalimidomethyl phthalolcyanine zinc, ZnPcS 2 P 2 (developed in China) was employed in the present study. According to previous studies, 22 ZnPcS 2 P 2 enjoys some advantages over conventional photosensitizers such as hematoporphyrin derivatives or other porphyrin derivatives. Our previous study 21 demonstrated for the first time that leukemic cell lines exhibited higher susceptibility to ZnPcS 2 P 2 -based PDT (ZnPcS 2 P 2 -PDT) than normal hematopoietic cells and ZnPcS 2 P 2 -PDT could eliminate the residual K562 cells in the simulated remission bone marrow.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

Huang

Chen

et al. 2005

Bone Marrow Transplant

View full text Add to dashboard Cite

A key point for successful transplantation of autologous hematopoietic stem cells in the treatment of leukemia is the purging technique, of which photodynamic therapy (PDT) proved effective and promising. The aim of this study was to evaluate the purging effect of a novel amphipathic photosensitizer, di-sulfo-di-phthalimidomethyl phthalolcyanine zinc (ZnPcS 2 P 2 )-based PDT (ZnPcS 2 P 2 -PDT) on murine erythroblastic leukemic EL9611 cells. Bone marrow cells (BMC), harvested from normal BALB/c mice, were contaminated with variable EL9611 cells. Cell suspensions were incubated with 4 lg/ml ZnPcS 2 P 2 for 5 h and then exposed to 2.1 J/cm 2 irradiation by a semiconductor laser 670 nm. Lethally irradiated recipient BALB/c mice (7 Gy) received syngeneic bone marrow transplantation with purged or nonpurged cell mixtures of 10 7 BMC contaminated with variable numbers (10 2 -10 5 ) of EL9611 cells. All of the irradiated controls died due to sepsis. All of the mice injected with nonpurged cell mixtures developed leukemia and died, whereas the mice transplanted with ZnPcS 2 P 2 -PDT-treated mixtures had a longer survival time, and the fewer leukemic cells there were in the cell mixtures, the higher the leukemia-free survival rate. We conclude that ZnPcS 2 P 2 -PDT could purge leukemic cells from bone marrow autografts but could retain sufficient progenitor cells for the hematopoietic activity.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

Huang

Chen

et al. 2005

Bone Marrow Transplant

View full text Add to dashboard Cite

show abstract

“…Photosensitizers are key factors for PDT. According to previous studies [9,10] , ZnPcS 2 P 2 presents 4 advantages over conventional photosensitizers such as hematoporphyrin derivatives (HpD) or other porphyrin derivatives. The first advantage is that the wavelength of suitable exicited light for ZnPcS 2 P 2 is 670 nm, which is good for penetrating into tissues.…”

Section: Introductionmentioning

confidence: 99%

Mitochondria-dependent apoptosis induced by a novel amphipathic photochemotherapeutic agent ZnPcS2P2 in HL60 cells1

Huang

Chen

2005

Acta Pharmacologica Sinica

View full text Add to dashboard Cite

Aim: To investigate the mechanism underlying the killing effects of a novel amphipathic photosensitizer, disulfonated diphthalimidomethyl phthalocyanine zinc (ZnPcS2P2), mediated photodynamic therapy (ZnPc‐PDT) in human myelogenous leukemia HL60 cells. Methods: After incubation for 5 h with 0.5 μmol/L ZnPcS2P2, the HL60 cells were exposed to a light source of 670 nm wavelength. Thereafter, the cells were detected at different time intervals after PDT. The characteristics of apoptosis were detected by observation of ultrastructure assay, DNA fragmentation assay and terminal deoxynucleotidyl transferase deoxyuridine nick‐end labeling method (TUNEL). Mitochondria‐dependent apoptosis was determined by the detection of mitochondrial membrane potential (Av|/m), activities of caspase family protease and of caspase‐3, cytosol cytochrome c. Proteins Bcl‐2 and Bax were detected by immunoblot analysis. Results: Evident characteristics of apoptosis were observed post‐ZnPc‐PDT with ultrastructure assay, DNA fragmentation assay and TUNEL staining. TUNEL assay showed that apoptotic rates in the cells collected from 6 h, 12 h and 24 h after PDT were 9. 6%, 24.4%, and 33.0%, respectively. HL60 cells underwent mitochondria‐dependent apoptosis as a result of cytochrome c release from mitochondria into cytosol accompanied by a reduction of Δψm. The activities of caspase family protease and of caspase‐3 were elevated. Furthermore, ZnPc‐PDT could remarkably down‐regulate the Bcl‐2 pro‐apoptotic protein and up‐regulate the anti‐apoptotic Bax protein. Conclusion: ZnPc‐PDT could induce mitochondria‐dependent apoptosis in HL60 cells.

show abstract

“…[10–13] A recent example is zinc disulfo-di(phthalimidomethyl)phthalocyanine (ZnPc-S 2 P 2 ), which was shown to be effective at killing tumor cells in vitro[14,15] and causing tumor regression in vivo. [16] A phase I clinical trial for this amphipathic photosensitizer is currently underway. [17] Another disulfonated derivative, disulfonated aluminum phthalocyanine (AlPcS 2 ), has also been shown to induce tumor regression mainly through direct killing of tumor cells rather than damaging the tumor vasculature.…”

Section: Introductionmentioning

confidence: 99%

Pentalysine β‐Carbonylphthalocyanine Zinc: An Effective Tumor‐Targeting Photosensitizer for Photodynamic Therapy

et al. 2010

View full text Add to dashboard Cite

Unsymmetrical phthalocyanine derivatives have been widely studied as photosensitizers for photodynamic therapy (PDT), targeting various tumor types. However, the preparation of unsymmetrical phthalocyanines is always a challenge due to the presence of many possible structural isomers. Herein we report a new unsymmetrical zinc phthalocyanine, pentalysine β-carbonylphthalocyanine zinc (ZnPc-(Lys) 5 ), that was prepared in large quantity and high purity. This is a water-soluble cationic photosensitizer and maintains a high quantum yield of singlet oxygen generation similar to that of unsubstituted zinc phthalocyanine (ZnPc). Compared with anionic ZnPc counterparts, ZnPc-(Lys) 5 shows a higher level cellular uptake and 20-fold higher phototoxicity toward tumor cells. Pharmacokinetics and PDT studies of ZnPc-(Lys) 5 in S180 tumor-bearing mice showed a high ratio of tumor versus skin retention and significant tumor inhibition. This new molecular framework will allow synthetic diversity in the number of lysine residues incorporated and will facilitate future QSAR studies.

show abstract

Metal phthalocyanine as photosensitizer for photodynamic therapy (PDT)

Cited by 44 publications

References 3 publications

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

Purging of murine erythroblastic leukemia by ZnPcS2P2-based-photodynamic therapy

Mitochondria-dependent apoptosis induced by a novel amphipathic photochemotherapeutic agent ZnPcS2P2 in HL60 cells1

Pentalysine β‐Carbonylphthalocyanine Zinc: An Effective Tumor‐Targeting Photosensitizer for Photodynamic Therapy

Contact Info

Product

Resources

About