Metal–organic frameworks-based biosensor for sequence-specific recognition of double-stranded DNA

Abstract

A simple, cost-efficient, sensitive and selective fluorescence sensor is developed for sequence-specific recognition of duplex DNA (ds-DNA) in vitro using metal-organic framework (MOF) as the sensing platform. N,N-Bis(2-hydroxy-ethyl)dithiooxamidatocopper(II) (H(2)dtoaCu) was chosen as the example MOF, because it strongly chemisorbs the dye-labeled probe TFO (triplex-forming oligonucleotide), and quenches fluorescence from the dye. In the presence of target ds-DNA (the PPT of HIV RNA, a 16-bp ds-DNA sequence), the TFO could interact with the major groove in ds-DNA (via Hoogsteen hydrogen bonding) to form a rigid triplex structure, resulting in fluorescence recovery. The enhanced fluorescence signal has a relationship with the ds-DNA concentration, the detection limit is as low as 1.3 nmol L(-1) (S/N = 3) with good selectivity, which is lower than that based on a graphene oxide platform and electrochemical-DNA sensor.

Sensing-functional luminescent metal–organic frameworks

“…78 Many examples of luminescent MOFs have been reported for sensing of biomolecules. [79][80][81][82][83][84] The recognition and detection of salicylaldehyde (SA) was demonstrated by Duan et al 79 80 Kumar et al realized the sensing of Bovine serum albumin (BSA) in an aqueous medium by using the MOFs tagged with an anti-BSA antibody. 81 In addition, Lin et al employed the MOF Cu(H 2 dtoa) (H 2 dtoa = Dithiooxamide) as a sensing platform which can adsorb probe DNA and release it upon the addition of target molecules, thus causing the fluorescence change.…”

“…In their another demonstration, they also showed that the detection limit of HIV DNA can be raised to 1.3 nM by using the MOF Cu(H 2 dtoa) containing dye-labeled probe triplex-forming oligonucleotide (TFO) as the sensor. 83 Furthermore, Ruan et al designed two luminescent MOFs Cd(L)•(HDMA) 2 (DMF)(H 2 O) 3 and Zn(L)•(HDMA) 2 (DMF)(H 2 O) 6 (L = bis-(3,5-dicarboxy-phenyl)terephthalamide, HDMA = protonated dimethylamine) that can be utilized as sensing platform for DNA stands. 84 Such examples suggest that novel MOF platforms should be excellent candidates for detection of biomolecules.…”

DNA Detection by Flow Cytometry using PNA‐Modified Metal–Organic Framework Particles

“…Chen et al 11b. developed a selective fluorescence sensor for sequence‐specific recognition of HIV ds‐DNA in vitro, using a MOF functionalized with triplex‐forming oligonucleotide (TFO) probes as the sensing platform.…”

Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework

“…Among them, research as biosensors based on MOF has become more and more attractive and made great progress in recent years 26 . For examples, Chen’s group utilized MOF as the sensing platform to detect ds-DNA with the detection limit 1.3 nM 27 ; Kang’s group presented a novel sensing strategy based on peptide nucleic acid probes labeled with fluorophores and conjugated with an NMOF vehicle to monitor multiplexed miRNAs in living cancer cells 28 ; Tang’s group developed a novel heterogeneous nano-MOF probe for H 2 S detection in HepG2 cells 29 . In contrast, MOF as electrochemical biosensors are rarely reported, which is usually caused by its low electro-activity.…”