Metal–Organic Enzyme Nanogels as Nanointegrated Self-Reporting Chemobiosensors

Abstract

A fluorometric glucose biosensor based on fine-tuned chemoenzymatic nanohybrids is herein proposed. The successful integration of an engineered glucose oxidase enzyme and an optically responsive polymeric nanogel in a single entity has led to the fabrication of a highly efficient glucose chemobiosensor. The optical responsiveness has been achieved by the loading of preactivated polymeric hydrogel with fluorescent lanthanide, i.e., cerium (III), cations. A comprehensive investigation of the responsiveness of the biomaterial revealed the interplay between the oxidation state of the cerium lanthanide and the fluorescence emission of the polymer. Finally, a full structural, chemical, and biochemical characterization of the reported system supports the chemobiosensors as robust, specific, and sensitive materials that could be utilized to faithfully quantify the amount of glucose in tear fluids.

Review on Healthcare Biosensing Nanomaterials

“…This sensor exhibited a wide linear range from 1 × 10 −9 to 1 × 10 −3 mg/mL at 30 °C. On the other hand, a supramolecular system comprising GOx enzyme and Ce(III) was integrated within a novel nanogel entity 69 to selectively detect and quantify glucose throughout optical readout (LOD = 73.37 nM).…”

Tetra-Nuclear Cluster-Based Lanthanide Metal–Organic Frameworks as White Phosphor, Information Encryption, Self-Calibrating Thermometers, and Fe2+ Sensors

“…Therefore, real-time monitoring of Fe 2+ in the water environment is very important. To detect low-concentration Fe 2+ , lanthanide-based materials and their sensing devices are promising candidates. − The rapid development of miniature sensing devices has attracted much attention because of their fast response, excellent visibility, high sensitivity, and high selectivity for detection. A variety of miniature chemical sensing devices have emerged including thin films, agarose gels, electrodeposition preparation of FTO/ITO films, dipsticks, and so on .…”

A self-cascade system based on Ag nanoparticle/single-walled carbon nanotube nanocomposites as an enzyme mimic for ultrasensitive detection of l-cysteine

“…Currently, a series of nanomaterials like carbon-based nanomaterials, 17,18 metal oxide-based nanomaterials, 19–21 metal organic frameworks, 22,23 and metal-based nanomaterials 24,25 have been proven to possess unique enzyme-mimicking catalytic activities, resembling those of peroxidase, oxidase, catalase (CAT), or superoxide dismutase (SOD). Importantly, various nanomaterials can be assembled into functional cascade systems widely used in detecting diverse analytes.…”