Metal ions requirement of polynucleotide phosphorylase

Synthesis of Polynucleotides by Microorganisms

“…Babinet et a1.,5) however, demonstrated that various di valent metal ions were capable of replacing Mg2+ in the phosphorolysis of polynucleotides. This fact would be supported by the present experiments, in which Mg2+ was demonstrat ed to be replaced by Mn2+ or Colt in the synthesis of polynucleotides by bacterial liv ing cells or their extracts, although there are subtle differences between the results of Babinet et al 5) and the present results in the optimal ratio of substrate concentration to divalent ion concentration. The investigation of the differences by using highly purified enzyme preparation must be the subject of future research.…”

Synthesis of Polynucleotides by Microorganisms

“…Babinet et al,5J however, demonstrated that various divalent metal ions were capable of replacing MgH in the phosphorolysis of polynucleotides. This fact would be supported by the present experiments, in which MgH was demonstrated to be replaced by Mn H or COH in the synthesis of polynucleotides by bacterial living cells or their extracts, although there are subtle differences between the results of Babinet et al 5 ) and the present results in the optimal ratio of substrate concentration to divalent ion concentration. The investigation of the differences by using highly purified enzyme preparation must be the subject of future research.…”

Partial purification and properties of rat liver mitochondrial polynucleotide phosphorylase

“…130 tion of acid-insoluble radioactive products from "'C-labelled nucleoside diphosphates: (i) 1-5mM-ADP, 5mM-CDP, 5mM-UDP; (ii) 1-8mM-MgCl2 and 0.2-8mM-MnCI2 (cf. Babinet et al, 1965); (iii) pH7.4, 7.8, 8.2, 8.6 and 9.0; (iv) the addition of (a) bovine serum albumin (100 and 200,ug/ml), (b) cyclic AMP (adenosine 3': 5'-cyclic monophosphate) (5,umol/ ml), (c) ApApA (100 and 200,g/ml), (d) oligo-and 40 36 32 poly-lysines and polyornithine (cf. Fitt & Wille, 1969), (e) lOmM-NaCl, (f) Triton X-100 or cetyltrimethylammonium bromide.…”