Metal Ions Impact on <i>Shewanella Oneidensis</i> MR-1 Adhesion to ITO Electrode and the Enhancement of Current Output

Abstract

The goal of this study is to enhance the efficiency of bacterial extracellular electron transfer (EET) in Shewanella oneidensis MR-1 by enhancing adhesion to the electrode surface. Our results clearly show a major difference in attachment and behavior of S. oneidensis MR-1 for Ca 2+ , Pb 2+ , Cd 2+ , and Mg 2+ compared to the control. The final microbial coverage, as measured by confocal microscopy and cathodic peak charge in cyclic voltammetry (Q pc ), increases with increasing metal ion concentrations. We found the cells attached to the electrode increased more with the addition of metal ion concentrations in the following order of metals: Ca 2+ > Pb 2+ > Cd 2+ > Mg 2+ compared to the control. The effect of metal ions on metabolism of the bacteria was tested by the riboflavin production and glucose consumption. Metabolic activity mirrored the same order of the activity as the electrochemical results.

Effect of micronutrient addition and development on microbial fuel cells (MFC) from food waste with the help of hydrolytic fungi

“…Increased biofilm formation is a general protective mechanism for bacteria when exposed heavy metal ions, because the biofilm can withstand higher toxicity. Anodic biofilm, as a bacterial community attached to the anode of MFC, also plays an important role for power generation in MFC [2]. As shown in Figure 5, the number of control variable cells (without the addition of micronutrients) was less, this was because there were only a few bacteria attached to the anodic electrode surface due to the low ability of S. oneidensis MR-1 biofilm formation.…”

“…MFCs have several advantages over conventional treatment technologies, including producing activated sludge in small quantities compared to using anaerobic digestion and conventional aerobic activated sludge (CAAS) [1]. The performance of the MFC in generating electrical energy is influenced IOP Publishing doi:10.1088/1755-1315/1108/1/012005 2 by several factors, such as the type of electrode, the use of a catalyst at the electrode, the type of microbe used, the type of contaminant being degraded, the type of configuration, the type of separator used and the operating conditions of the MFC [2]. In this research used carbon cloth, where the type of electrode has several advantages including high porosity, has a large specific area a good mechanical strength, flexible and a high conductivity level [1].…”