Metal Ion–Enhanced Quantification of Chloramphenicol in Milk Using Imipramine Hydrochloride as Diazo-Coupling Agent

Sharma, Ritu; Krishna, Honnur; Raghavarao, K.S.M.S.

doi:10.1007/s12161-020-01837-w

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…Wafi, et al, [22] described a spectrophotometric method with a diazotization reaction at room temperature to analyze shrimp with a LOD of 0.36 μg/mL and a LOQ of 1.19 μg/mL. Sharma, et al, [10] detected chloramphenicol residues in milk with a diazotization reaction at room temperature resulting in a %recovery of 98.66-101.12%. Hussein, et al, [23] analyzed using an oxidative coupling reaction to produce LOD, LOQ, and %recovery of 0.241 μg/mL, 0.804 μg/mL, and 99.77-100.5%, respectively.…”

Section: Formation An Azo Dyementioning

confidence: 99%

“…The standard addition method was chosen because the sample is added to a certain quantity of standard solution whose concentration is known accurately so that the sample's matrix does not influence the analysis. The diazotization reaction was chosen because it is simpler, more accurate, and cheaper in detecting CAP by producing an intensely colored azo dye solution that produces a maximum absorption at a wavelength at 575 nm [10]. Ravisankar [11] analyzed azo dye at a wavelength at 550 nm.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Almira¹

2022

ijcsrr

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This research aimed to determine the concentration of chloramphenicol in shrimp using the standard addition method based on the diazotization reaction using Zn powder as a reducing agent of chloramphenicol, followed by the use of N-(1-naphthyl) ethylenediamine dihydrochloride as a coupling agent and measured at 565 nm. Based on the test, the shrimp sample was found to contain 1964.91 mg/kg of chloramphenicol and it exceeded the requirements set by the European Commission which was 0,15 mg/kg. The limit of detection (LOD) value is 0.19 mg/mL and the limit of quantitation (LOQ) value is 0.64 mg/mL. The correlation coefficient (R2) was 0.9991 for the concentration range of 0-50 ppm. The analysis of the results showed that the %recovery in shrimp analysis using this method was 87.41%-107.73% with an average of 109.38%.

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Section: Formation An Azo Dyementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Almira¹

2022

ijcsrr

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“…Chloramphenicol (CAP) is a broad-spectrum antibiotic, exhibiting activity against both Gram-positive and Gram-negative bacteria as well as other groups of microorganisms. [1][2][3] The abuse of antibiotics causes negative effects on food safety and human health. 4,5 Many studies have reported that many diseases including blood dyscrasias, 6 aplastic anaemia, 7 and gray baby syndrome 8 are associated with the abuse of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Sensitive fluorescence assay of chloramphenicol coupled with two-level isothermal amplification using a self-powered catalyzed hairpin assembly and entropy-driven circuit

Xiong,

Wang,

Liu

et al. 2024

Anal. Methods

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“…However, there are several disadvantages to chloramphenicol analysis using HPLC, such as the high cost, infrequent HPLC instruments, complicated procedures, and long waiting time, so it is necessary to develop analytical methods that are simple to observe, quick, and inexpensive. One method that is fast and easy to observe is the photometric reaction-based method because it produces a colored solution that is easy to be observed [12]. The chemical structure of chloramphenicol is as follows:…”

Section: Introductionmentioning

confidence: 99%

Determination of the optimum concentration of the coupling agent in chloramphenicol analysis

Khudzaifah¹,

Basukiwardojo²

2022

World J. Adv. Res. Rev.

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The aim of this research is to determine the optimum concentration of the coupling agent used in chloramphenicol analysis, then calculate the limit of detection of that photometric reaction. Chloramphenicol is a colorless compound that is often abused in animals whose products are consumed by humans. Chloramphenicol has side effects on the human body. So, chloramphenicol must be analyzed. To facilitate the analysis process, chloramphenicol must be converted into colored compounds called azo compounds. To make azo compounds must go through the process of reduction, diazotization, and coupling. A coupling agent is an important compound that played a role in an azo compound formation and was analyzed using UV-Vis spectrophotometer. This research, used N-(-1-Naphthyl) ethylenediamine dihydrochloride (NEDA) with variations concentrations of 0.1%, 0.3%, 0.5% at 565 nm. The optimum coupling concentration that gives maximum absorbance was 0.1%. The detection limit (LOD) obtained by using the optimum coupling is 0.0498 μg/mL, the limit of quantitation (LOQ) is 0.1660 μg/mL, the sensitivity value is 0.1425, and a correlation coefficient (R2) of 0.999 with a concentration range of chloramphenicol is 0,14 - 1,4 ppm.

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Metal Ion–Enhanced Quantification of Chloramphenicol in Milk Using Imipramine Hydrochloride as Diazo-Coupling Agent

Cited by 5 publications

References 20 publications

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Sensitive fluorescence assay of chloramphenicol coupled with two-level isothermal amplification using a self-powered catalyzed hairpin assembly and entropy-driven circuit

Determination of the optimum concentration of the coupling agent in chloramphenicol analysis

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