Metal-ion dependent DNAzyme recycling amplification for sensitive and homogeneous immuno-proximity binding assay of α-fetoprotein biomarker

Zou, Mengqi; Li, Daxiu; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.bios.2016.10.044

Cited by 36 publications

(19 citation statements)

References 32 publications

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“…The proximity hybridization assay (PHA), first reported by Landegren and co-workers in 2002, is attracting more and more attention in the clinical diagnosis of proteins. In PHA, a protein-recognition event is converted into DNA detection. , The PHA with good performance has been applied for the detection of proteins, such as alpha-fetoprotein (AFP), , carcinoembryonic antigen (CEA), − total protein of nosema bombycis, prostate-specific antigen, thrombin, − platelet-derived growth factor BB, − insulin, concanavalin A, O-GlcNAcylated proteins, cystatin C, and prostasomes . In the reported PHA, a number of DNA-based amplification strategies, such as polymerase chain reaction, ,,, endonuclease-assisted recycling amplification, − ,, localized rolling-circle amplification, , and nanoparticles- based amplification strategies, ,,, have been employed to enhance the sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…In PHA, a protein-recognition event is converted into DNA detection. , The PHA with good performance has been applied for the detection of proteins, such as alpha-fetoprotein (AFP), , carcinoembryonic antigen (CEA), − total protein of nosema bombycis, prostate-specific antigen, thrombin, − platelet-derived growth factor BB, − insulin, concanavalin A, O-GlcNAcylated proteins, cystatin C, and prostasomes . In the reported PHA, a number of DNA-based amplification strategies, such as polymerase chain reaction, ,,, endonuclease-assisted recycling amplification, − ,, localized rolling-circle amplification, , and nanoparticles- based amplification strategies, ,,, have been employed to enhance the sensitivity. However, the involvement of ligase and polymerase in DNA amplifications and the complicated synthesis of nanoparticles in nanoparticle amplifications truly increased the cost and complexity of these detection methods.…”

Section: Introductionmentioning

confidence: 99%

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Proximity Hybridization-Regulated Immunoassay for Cell Surface Protein and Protein-Overexpressing Cancer Cells via Electrochemiluminescence

Wang

Gao

et al. 2018

Anal. Chem.

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A simple electrochemiluminescence (ECL) immunoassay based on a proximity hybridization-regulated strategy was developed for highly sensitive and specific detection of cell surface protein and protein-overexpressing cancer cells. A biosensor was fabricated by self-assembling a thiolated capture ss-DNA3 (partially hybridize with ss-DNA1 and ss-DNA2) and blocking with 6-mercapto-1-hexanol on a gold electrode surface. Target protein was simultaneously bound by two ss-DNA-tagged antibody probes (DNA1-Ab1 and DNA2-Ab2), while DNA1 and DNA2 were brought in sufficient proximity and hybridized with capture DNA3 on the surface of the biosensor. After ECL signal reagent Ru(phen) was intercalated into the hybridized ds-DNAs, ECL measurement was performed in the coreactant solution. A "signal on" proximity hybridization-regulated ECL immunoassay for alpha-fetoprotein (AFP) was developed. The ECL intensity increased with the increase of AFP concentration in the range of 0.05-20.0 ng/mL with a detection limit of 6.2 pg/mL. Moreover, the developed ECL method was successfully used to detect AFP-overexpressing cancer cells (MCF-7 cancer cells as model) with a detection limit of 620 cells/mL (∼60 MCF-7 cells in 100 μL of cell suspension) and discriminate AFP expression on different types of the living cell surface. This work for the first time reports a proximity hybridization-regulated ECL immunoassay for the detection of the cell surface protein on a living cell surface with good specificity and sensitivity. This simple, specific, and sensitive strategy is greatly promising for the detection of proteins and specific cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proximity Hybridization-Regulated Immunoassay for Cell Surface Protein and Protein-Overexpressing Cancer Cells via Electrochemiluminescence

Wang

Gao

et al. 2018

Anal. Chem.

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“…To achieve this, we engineer the DNAzyme to adapt into binding-induced DNA assembly (BINDA) and build a target-triggered DNAzyme motor that autonomously traverses along AuNPs. Inspired by the proximity ligation assay, − we recently proposed a new concept of DNA assembly termed BINDA, which enables protein binding to trigger the assembly of separate DNA components that are otherwise unable to spontaneously assemble. − Additionally, the use of DNAzyme allows us to rationally design the binding arms of the DNAzyme motor, enabling the motor to maintain similar walking activity at different temperatures. The DNAzyme motor also has the advantage of easy control, as a simple addition or depletion of the cofactor Mg 2+ can switch it on or off.…”

mentioning

confidence: 99%

A Target-Triggered DNAzyme Motor Enabling Homogeneous, Amplified Detection of Proteins

et al. 2017

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We report here the concept of a self-powered, target-triggered DNA motor constructed by engineering a DNAzyme to adapt into binding-induced DNA assembly. An affinity ligand was attached to the DNAzyme motor via a DNA spacer, and a second affinity ligand was conjugated to the gold nanoparticle (AuNP) that was also decorated with hundreds of substrate strands serving as a high-density, three-dimensional track for the DNAzyme motor. Binding of a target molecule to the two ligands induced hybridization between the DNAzyme and its substrate on the AuNP, which are otherwise unable to spontaneously hybridize. The hybridization of DNAzyme with the substrate initiates the cleavage of the substrate and the autonomous movement of the DNAzyme along the AuNP. Each moving step restores the fluorescence of a dye molecule, enabling monitoring of the operation of the DNAzyme motor in real time. A simple addition or depletion of the cofactor Mg allows for fine control of the DNAzyme motor. The motor can translate a single binding event into cleavage of hundreds of substrates, enabling amplified detection of proteins at room temperature without the need for separation.

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“…In addition to these novel nanoparticles, biomarker sensors can also be optimized by improving Ab2 decorated with other materials, such as polymers [ 95 ]. Zou et al proposed a new signal enhancement strategy for protein biomarkers assay binding based on a metal-ion-dependent DNAzyme recycling [ 96 ].…”

Section: Electrochemical Biomarker Sensorsmentioning

confidence: 99%

Recent Progress of Biomarker Detection Sensors

Liu

Cui

2020

Research

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Early cancer diagnosis and treatment are crucial research fields of human health. One method that has proven efficient is biomarker detection which can provide real-time and accurate biological information for early diagnosis. This review presents several biomarker sensors based on electrochemistry, surface plasmon resonance (SPR), nanowires, other nanostructures, and, most recently, metamaterials which have also shown their mechanisms and prospects in application in recent years. Compared with previous reviews, electrochemistry-based biomarker sensors have been classified into three strategies according to their optimizing methods in this review. This makes it more convenient for researchers to find a specific fabrication method to improve the performance of their sensors. Besides that, as microfabrication technologies have improved and novel materials are explored, some novel biomarker sensors—such as nanowire-based and metamaterial-based biomarker sensors—have also been investigated and summarized in this review, which can exhibit ultrahigh resolution, sensitivity, and limit of detection (LoD) in a more complex detection environment. The purpose of this review is to understand the present by reviewing the past. Researchers can break through bottlenecks of existing biomarker sensors by reviewing previous works and finally meet the various complex detection needs for the early diagnosis of human cancer.

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Metal-ion dependent DNAzyme recycling amplification for sensitive and homogeneous immuno-proximity binding assay of α-fetoprotein biomarker

Cited by 36 publications

References 32 publications

Proximity Hybridization-Regulated Immunoassay for Cell Surface Protein and Protein-Overexpressing Cancer Cells via Electrochemiluminescence

Proximity Hybridization-Regulated Immunoassay for Cell Surface Protein and Protein-Overexpressing Cancer Cells via Electrochemiluminescence

A Target-Triggered DNAzyme Motor Enabling Homogeneous, Amplified Detection of Proteins

Recent Progress of Biomarker Detection Sensors

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