Metal-containing systems based on chitosan and a collagen-chitosan composite

́kov, A. Yu. Vasil; Rubina, Margarita S.; Naumkin, A. V.; Zubavichus, Ya. V.; Belyakova, O. A.; Maksimov, Yu. V.; Imshennik, V. K.

doi:10.1007/s11172-015-1057-5

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…The AuNPs deposited in the pristine soybean-based fabric showed two low intensity peaks at 87.2 and 83.6 eV, which are assigned to Au4f 5/2 and Au4f 7/2 spin-orbit coupling of bulk metallic Au 0 interacting with the carboxylic groups or the protein amino/peptide functionalities of soybean fibres [54]. This is suggested by the smaller value of binding energy of Au4f 7/2 in respect of the characteristic parameter for bulk Au (84 eV) [55]. The addition of chitosan to the system clearly shows that the AuNPs were partially oxidized on the fibre surface.…”

Section: Resultsmentioning

confidence: 99%

Multifunctional Chitosan/Gold Nanoparticles Coatings for Biomedical Textiles

et al. 2019

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Gold nanoparticles (AuNPs), chemically synthesized by citrate reduction, were for the first time immobilized onto chitosan-treated soybean knitted fabric via exhaustion method. AuNPs were successfully produced in the form of highly spherical, moderated polydisperse, stable structures. Their average size was estimated at ≈35 nm. Successful immobilization of chitosan and AuNPs were confirmed by alterations in the fabric’s spectrophotometric reflectance spectrum and by detection of nitrogen and gold, non-conjugated C=O stretching vibrations of carbonyl functional groups and residual N-acetyl groups characteristic bands by X-ray photoelectron spectroscopy (XPS) and Fourier-Transform Infrared Spectroscopy (FTIR) analysis. XPS analysis confirms the strong binding of AuNPs on the chitosan matrix. The fabrics’ thermal stability increased with the introduction of both chitosan and AuNPs. Coated fabrics revealed an ultraviolet protection factor (UPF) of +50, which established their effectiveness in ultraviolet (UV) radiation shielding. They were also found to resist up to 5 washing cycles with low loss of immobilized AuNPs. Compared with AuNPs or chitosan alone, the combined functionalized coating on soy fabrics demonstrated an improved antimicrobial effect by reducing Staphylococcus aureus adhesion (99.94%) and Escherichia coli (96.26%). Overall, the engineered fabrics were confirmed as multifunctional, displaying attractive optical properties, UV-light protection and important antimicrobial features, that increase their interest for potential biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Multifunctional Chitosan/Gold Nanoparticles Coatings for Biomedical Textiles

et al. 2019

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“…The black organosol was instantly discolored and the embedding of chitosan with metal NPs was occurred immediately. More detailed information about MVS set up and procedure reported elsewhere [17]. On the third step, the solvent was removed and the metal-carrying chitosan powder was dried in a vacuum of 1 Pa at 60°C during 3 h. All manipulations were performed in a pure Ar atmosphere.…”

Section: Preparation Of Cu@chit Nanocompositesmentioning

confidence: 99%

Synthesis and characterization of chitosan–copper nanocomposites and their fungicidal activity against two sclerotia-forming plant pathogenic fungi

et al. 2017

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In this report, the metal-vapor synthesis (MVS) was used for the preparation of copper nanoparticles which was then used for the preparation of chitosan-copper nanocomposite. The antifungal activity of Cu@Chit NCs against two sclerotium-forming plant pathogenic fungi Sclerotium rolfsii (S. rolfsii) and Rhizoctonia solani (R. solani) AG-4 was evaluated in vitro and their effects on hyphal morphology, and sclerotia formation were observed for the first time. The NCs were prepared through impregnation of chitosan with colloid solution of copper nanoparticles in organic solvent (acetone or toluene). Transmission electron microscopy shows that the particles have predominantly spherical form, polydisperse character, the mean diameter about 2-3 nm and a rather uniform distribution in the chitosan matrice. Analysis of the small angle scattering curves suggests that the copper particles in the NCs with the size of B2 nm are mostly located in the chitosan pores with the same size. The effect of Cu@Chit NCs on fungal growth reveals some significant inhibitory activity against two tested fungi. The highest level of inhibition against S. rolfsii and R. solani AG-4 was observed using the high concentrations of Cu@Chit NC prepared using acetone as a solvent. A loss of the cytoplasm content, cytoplasmic coagulation, irregular shape of mycelia, or destruction in the hyphae was confirmed. The experiments demonstrate that the Cu@Chit NC synthesized via MVS using acetone was more effective than that of toluene in inhibiting fungal hyphae growth against R. solani AG-4 and S. rolfsii. The results show that the Cu@Chit NCs are fungicidal against both the tested fungus at high concentrations and the fungicidal or fungistatic activity is dependent on the tested fungus species.

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“…Chitosan powder decorated with Cu NPs was prepared according to the procedure described in the previously published works [ 17 , 18 , 19 , 20 ]. For the preparation of organosol, 0.56 g of Cu foil (about 200 µm of thickness) was resistively dispersed from the tantalum boat (90 mm × 5 mm) and co-condensed with 160 mL of acetone on the liquid nitrogen-cooled walls of a quartz 5 L vessel.…”

Section: Methodsmentioning

confidence: 99%

Copper-Chitosan Nanocomposite Hydrogels Against Aflatoxigenic Aspergillus flavus from Dairy Cattle Feed

Abd-Elsalam

Alghuthaymi

Shami

et al. 2020

JoF

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The integration of copper nanoparticles as antifungal agents in polymeric matrices to produce copper polymer nanocomposites has shown excellent results in preventing the growth of a wide variety of toxigenic fungi. Copper-chitosan nanocomposite-based chitosan hydrogels (Cu-Chit/NCs hydrogel) were prepared using a metal vapor synthesis (MVS) and the resulting samples were described by transmission electron microscopy (TEM), X-ray fluorescence analysis (XRF), and small-angle X-ray scattering (SAXS). Aflatoxin-producing medium and VICAM aflatoxins tests were applied to evaluate their ability to produce aflatoxins through various strains of Aspergillus flavus associated with peanut meal and cotton seeds. Aflatoxin production capacity in four fungal media outlets revealed that 13 tested isolates were capable of producing both aflatoxin B1 and B2. Only 2 A. flavus isolates (Af11 and Af 20) fluoresced under UV light in the A. flavus and parasiticus Agar (AFPA) medium. PCR was completed using two specific primers targeting aflP and aflA genes involved in the synthetic track of aflatoxin. Nevertheless, the existence of aflP and aflA genes indicated some correlation with the development of aflatoxin. A unique DNA fragment of the expected 236 bp and 412 bp bands for aflP and aflA genes in A. flavus isolates, although non-PCR fragments have been observed in many other Aspergillus species. This study shows the antifungal activity of Cu-Chit/NCs hydrogels against aflatoxigenic strains of A. flavus. Our results reveal that the antifungal activity of nanocomposites in vitro can be effective depending on the type of fungal strain and nanocomposite concentration. SDS-PAGE and native proteins explain the apparent response of cellular proteins in the presence of Cu-Chit/NCs hydrogels. A. flavus treated with a high concentration of Cu-Chit/NCs hydrogels that can decrease or produce certain types of proteins. Cu-Chit/NCs hydrogel decreases the effect of G6DP isozyme while not affecting the activity of peroxidase isozymes in tested isolates. Additionally, microscopic measurements of scanning electron microscopy (SEM) showed damage to the fungal cell membranes. Cu-Chit/NCS hydrogel is an innovative nano-biopesticide produced by MVS is employed in food and feed to induce plant defense against toxigenic fungi.

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Metal-containing systems based on chitosan and a collagen-chitosan composite

Cited by 11 publications

References 28 publications

Multifunctional Chitosan/Gold Nanoparticles Coatings for Biomedical Textiles

Multifunctional Chitosan/Gold Nanoparticles Coatings for Biomedical Textiles

Synthesis and characterization of chitosan–copper nanocomposites and their fungicidal activity against two sclerotia-forming plant pathogenic fungi

Copper-Chitosan Nanocomposite Hydrogels Against Aflatoxigenic Aspergillus flavus from Dairy Cattle Feed

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