Metal complexes stability constant determination by hyphenation of capillary electrophoresis with inductively coupled plasma mass spectrometry: The case of 1:1 metal-to-ligand stoichiometry

Petit, Jérémy; Geertsen, Valérie; Beaucaire, Catherine; Stambouli, Moncef

doi:10.1016/j.chroma.2009.02.094

Cited by 32 publications

(36 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During each separation, it could be assumed that only tin compound and the complex exist in the running buffer. Additionally, in the previous published papers, CE-ICP-MS has been applied successfully to investigate the stability constant determination of lanthanum-oxalate and uranyl-oxalate complexes in the assumption of 1:1 metal-to-ligand stoichiometry [10,27]. Thus, for simple calculation, the interaction of protein-organotin compound was assumed to be 1:1 complex molar ratio in described study firstly.…”

Section: Resultsmentioning

confidence: 99%

Characterization of interactions between organotin compounds and human serum albumin by capillary electrophoresis coupled with inductively coupled plasma mass spectrometry

Sun

Liu

et al. 2012

Talanta

View full text Add to dashboard Cite

Inductively coupled plasma mass spectrometry Human serum albumin Affinity capillary electrophoresis Nonequilibrium capillary electrophoresis assays of equilibrium mixtures assays a b s t r a c t Thermodynamic data such as the binding constants are vital parameters describing interactions between exotic trace compounds and biomolecules in biochemical property modeling. In this study, the stability constants of organometallic compound and protein complexes were studied by using capillary electrophoresis coupled with inductively coupled plasma mass spectrometry (CE-ICP-MS), considering its low detection limits and low sample demand. Four organotin compounds (trimethyltin (TMT), tripropyltin (TPrT), tributyltin (TBT), triphenyltin (TPhT)) and human serum albumin (HSA) were used as model organometallic compounds and protein, respectively. Affinity capillary electrophoresis (ACE) and nonequilibrium capillary electrophoresis assays of equilibrium mixtures (NECEEM) were performed and compared by using ICP-MS as the detector to determine the binding constants of organotin compounds and HSA in 1:1 molar ratio assumption. Constant measurements of the two methods were both simple, however, ACE assays were more accurate and more appropriate for the constant determination of the organotin-HSA complexes, considering the errors of the NECEEM method. A good precision of the binding constants (log K b ) using the ACE method was proved by different mathematical calculations, and the values were 6.13 ± 0.51 (TMT), 5.72 ± 0.38 (TPrT), 5.68 ± 0.34 (TBT), 6.05 ± 0.38 (TPhT) respectively for each of the organotin-HSA complexes, showing non-covalent interaction between organotin compounds and HSA. Meanwhile, this study also confirms the suitability of CE-ICP-MS method for further studies on organometallic complexation.

show abstract

Section: Resultsmentioning

confidence: 99%

Characterization of interactions between organotin compounds and human serum albumin by capillary electrophoresis coupled with inductively coupled plasma mass spectrometry

Sun

Liu

et al. 2012

Talanta

View full text Add to dashboard Cite

show abstract

“…The speciation study resorts thus to hyphenated techniques [2,3]: the species-selectivity is achieved by chromatography or electrophoresis, whereas element-selectivity and sensitivity are obtained by atomic or MS. Among the available hyphenated techniques, recent improvements in CE-ICPMS hyphenation and developments of commercial interfaces allow CE-ICPMS to be regarded as an attractive technique for speciation studies [4][5][6][7][8], gathering many advantages: low sample and electrolyte consumption, better respect of metal speciation, high sensitivity, possibility of implementation in a radiological controlled area, etc. [9].…”

Section: Introductionmentioning

confidence: 99%

“…In CE, the most common method used to estimate labile complexes stability constants is named ACE [2,13] and involves measuring the electrophoretic mobility variation of a metal ion with the concentration of ligand in the buffered electrolyte. This method, developed to calculate the 1:1 metal-to-ligand ratio stability constants, has been described previously [5]. In a real sample matrix, it appears that metal speciation is made up of 1:1 metal-ligand complexes but also various superior order complexes.…”

Section: Introductionmentioning

confidence: 99%

“…In order to predict its behaviour, Sonke and Salters [12] have built a diagram representing the complex stability areas, according to their stability and kinetic rate constants. The lability of the uranyl-oxalate and the lanthanum-oxalate complexes has already been established [5]. In CE, the most common method used to estimate labile complexes stability constants is named ACE [2,13] and involves measuring the electrophoretic mobility variation of a metal ion with the concentration of ligand in the buffered electrolyte.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Stability constants determination of successive metal complexes by hyphenated CE‐ICPMS

et al. 2010

Self Cite

View full text Add to dashboard Cite

The study of radionuclides speciation requires accurate evaluation of stability constants, which can be achieved by CE-ICPMS. We have previously described a method for 1:1 metal complexes stability constants determination. In this paper, we present its extension to the case of successive complexations and its application to uranyl-oxalate and lanthanum-oxalate systems. Several significant steps are discussed: analytical conditions choice, mathematical treatment by non-linear regression, ligand concentration and ionic strength corrections. The following values were obtained: at infinite dilution, log(beta(1) degrees (UO(2)Oxa))=6.93+/-0.05, log(beta(2) degrees (UO(2)(Oxa)(2) (2-)))=11.92+/-0.43 and log(beta(3) degrees (UO(2)(Oxa)(3) (4-)))=15.11+/-0.12; log(beta(1) degrees (LaOxa(+)))=5.90+/-0.07, log(beta(2) degrees (La(Oxa)(2) (-)))=9.18+/-0.19 and log(beta(3) degrees (La(Oxa)(3) (3-)))=9.81+/-0.33. These values are in good agreement with the literature data, even though we suggest the existence of a new lanthanum-oxalate complex: La(Oxa)(3) (3-). This study confirms the suitability of CE-ICPMS for complexation studies.

show abstract

“…Traditionally, in order to study the complaxation of new synthesized ligand with metallic cations, potentiometry, paper electrophoresis, membrane permeation, affinity capillary electrophoresis, UV-vis and fluorescence spectroscopy, and mass spectrometry can be used [14][15][16][17]. In this study, UV-vis spectroscopy was chosen because of its simplicity, low cost and availability of the UV-vis spectrophotometer in most laboratories.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Determination of Stability Constants of a New Bis-1,2,4-triazole Ligand for Complexation with Zinc(II), Copper(II) and Nickel(II) in Acetonitrile

Sabzi¹,

Nikoo²,

Nikzad³

et al. 2012

AJAC

View full text Add to dashboard Cite

In this work, the synthesis and complexation properties of a new compound, 1,3-bis[5-(2-hydroxyphenyl)-4-phenyl-1,2,4-triazole-3-yl-thio]propane (BTP), towards certain transition metal ions, (M(II) where M = Zn, Cu, Ni) in acetonitrile is reported. A hard-modeling strategy was applied to UV-Visible spectroscopy data obtained from monitoring the reaction between BTP and the selected metal ions to determine the concentration profiles of each species and the corresponding stability constant(s) of the complex(es). The stability constants of complexes are always defined in terms of their free metal, free ligand and complexed forms. These constants are influenced by parameters such as the type of metal, ligand, counterion or solvent. In this study, the formation constants of the complexes were determined for the synthesized ligand with several metallic cations in acetonitrile solvent by UV-Vis spectrophotometry.

show abstract

Metal complexes stability constant determination by hyphenation of capillary electrophoresis with inductively coupled plasma mass spectrometry: The case of 1:1 metal-to-ligand stoichiometry

Cited by 32 publications

References 24 publications

Characterization of interactions between organotin compounds and human serum albumin by capillary electrophoresis coupled with inductively coupled plasma mass spectrometry

Characterization of interactions between organotin compounds and human serum albumin by capillary electrophoresis coupled with inductively coupled plasma mass spectrometry

Stability constants determination of successive metal complexes by hyphenated CE‐ICPMS

Synthesis and Determination of Stability Constants of a New Bis-1,2,4-triazole Ligand for Complexation with Zinc(II), Copper(II) and Nickel(II) in Acetonitrile

Contact Info

Product

Resources

About