Metal complex formation by nicotianamine, a possible phytosiderophore

Beneš, I.; Schréiber, Klaus; Ripperger, Helmut; Kircheiss, A.

doi:10.1007/bf01955293

Cited by 141 publications

(82 citation statements)

References 10 publications

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“…Most of the remainder of the higher shell contributions, in particular the peak at 2.7 to 3 Å , seems to be due to Cu binding to the nonproteogenic amino acid NA, which has been shown to have a very high affinity for Cu (Beneš et al, 1983), and already earlier works suggested, on a physiological basis, that NA is involved in Cu homeostasis (Pich and Scholz, 1996;Liao et al, 2000;Irtelli et al, 2009). The explanation of the features in our EXAFS spectra with carboxylate-bound NA seems more likely than the explanation by His, as seen by the statistical analysis of the fits.…”

Section: Namentioning

confidence: 99%

Complexation and Toxicity of Copper in Higher Plants. II. Different Mechanisms for Copper versus Cadmium Detoxification in the Copper-Sensitive Cadmium/Zinc Hyperaccumulator Thlaspi caerulescens (Ganges Ecotype)

et al. 2009

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The cadmium/zinc hyperaccumulator Thlaspi caerulescens is sensitive toward copper (Cu) toxicity, which is a problem for phytoremediation of soils with mixed contamination. Cu levels in T. caerulescens grown with 10 mM Cu 2+ remained in the nonaccumulator range (,50 ppm), and most individuals were as sensitive toward Cu as the related nonaccumulator Thlaspi fendleri. Obviously, hyperaccumulation and metal resistance are highly metal specific. Cu-induced inhibition of photosynthesis followed the "sun reaction" type of damage, with inhibition of the photosystem II reaction center charge separation and the water-splitting complex. A few individuals of T. caerulescens were more Cu resistant. Compared with Cu-sensitive individuals, they recovered faster from inhibition, at least partially by enhanced repair of chlorophyll-protein complexes but not by exclusion, since the content of Cu in their shoots was increased by about 25%. Extended x-ray absorption fine structure (EXAFS) measurements on frozen-hydrated leaf samples revealed that a large proportion of Cu in T. caerulescens is bound by sulfur ligands. This is in contrast to the known binding environment of cadmium and zinc in the same species, which is dominated by oxygen ligands. Clearly, hyperaccumulators detoxify hyperaccumulated metals differently compared with nonaccumulated metals. Furthermore, strong features in the Cu-EXAFS spectra ascribed to metal-metal contributions were found, in particular in the Cu-resistant specimens. Some of these features may be due to Cu binding to metallothioneins, but a larger proportion seems to result from biomineralization, most likely Cu(II) oxalate and Cu(II) oxides. Additional contributions in the EXAFS spectra indicate complexation of Cu(II) by the nonproteogenic amino acid nicotianamine, which has a very high affinity for Cu(II) as further characterized here.

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Section: Namentioning

confidence: 99%

Complexation and Toxicity of Copper in Higher Plants. II. Different Mechanisms for Copper versus Cadmium Detoxification in the Copper-Sensitive Cadmium/Zinc Hyperaccumulator Thlaspi caerulescens (Ganges Ecotype)

et al. 2009

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“…The important role of NA in mineral homeostasis may be one of the reasons for the large number of YS family members in the rice genome. NA is well known for its capacity to chelate a variety of minerals (Benes et al, 1983), being involved in the transport of copper in the phloem and possibly performing the same function for zinc, iron and manganese (Stephan and Scholtz et al, 1993). In this regard, it is reasonable to suggest that different YS paralogs may coordinate the transport of various NA-metal complexes.…”

Section: Multiplicity Of Transporters and Members In The Gene Familiesmentioning

confidence: 99%

Iron homeostasis related genes in rice

et al. 2003

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Iron is essential for plants. However, excess iron is toxic, leading to oxidative stress and decreased productivity. Therefore, plants must use finely tuned mechanisms to keep iron homeostasis in each of their organs, tissues, cells and organelles. A few of the genes involved in iron homeostasis in plants have been identified recently, and we used some of their protein sequences as queries to look for corresponding genes in the rice (Oryza sativa) genome. We have assigned possible functions to thirty-nine new rice genes. Together with four previously reported sequences, we analyzed a total of forty-three genes belonging to five known protein families: eighteen YS (Yellow Stripe), two FRO (Fe 3+ -chelate reductase oxidase), thirteen ZIP (Zinc regulated transporter / Iron regulated transporter Protein), eight NRAMP (Natural Resistance -Associated Macrophage Protein), and two Ferritin proteins. The possible cellular localization and number of potential transmembrane domains were evaluated, and phylogenetic analysis performed for each gene family. Annotation of genomic sequences was performed. The presence and number of homologues in each gene family in rice and Arabidopsis is discussed in light of the established iron acquisition strategies used by each one of these two plants.

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“…YS1 orthologs are therefore part of the primary uptake machinery specific to poaceae plants. Another class of YSL proteins, which are found in both monocots and dicots, are involved in the mobilization within the plant of metal ions, complexed either with PS or with NA, a precursor of PS with similar affinity for metals (Benes et al, 1983). Their expression is associated with vascular tissues where they are involved in the distribution of metals between organs (Koike et al, 2004;Le Jean et al, 2005;Waters et al, 2006;Zheng et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis YELLOW STRIPE LIKE4 and 6 Transporters Control Iron Release from the Chloroplast

et al. 2013

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In most plant cell types, the chloroplast represents the largest sink for iron, which is both essential for chloroplast metabolism and prone to cause oxidative damage. Here, we show that to buffer the potentially harmful effects of iron, besides ferritins for storage, the chloroplast is equipped with specific iron transporters that respond to iron toxicity by removing iron from the chloroplast. We describe two transporters of the YELLOW STRIPE1-LIKE family from Arabidopsis thaliana, YSL4 and YSL6, which are likely to fulfill this function. Knocking out both YSL4 and YSL6 greatly reduces the plant's ability to cope with excess iron. Biochemical and immunolocalization analyses showed that YSL6 resides in the chloroplast envelope. Elemental analysis and histochemical staining indicate that iron is trapped in the chloroplasts of the ysl4 ysl6 double mutants, which also accumulate ferritins. Also, vacuolar iron remobilization and NRAMP3/4 expression are inhibited. Furthermore, ubiquitous expression of YSL4 or YSL6 dramatically reduces plant tolerance to iron deficiency and decreases chloroplastic iron content. These data demonstrate a fundamental role for YSL4 and YSL6 in managing chloroplastic iron. YSL4 and YSL6 expression patterns support their physiological role in detoxifying iron during plastid dedifferentiation occurring in embryogenesis and senescence.

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Metal complex formation by nicotianamine, a possible phytosiderophore

Abstract: Summary, The acid dissociation constants of nicotianamine (1) (pK l = 6.97, pK2= 9.13, pK 3 = 9.75; 0.1 M KC104, 25 ~ and the stability constants for its 1:1 complexes with bivalent metal ions (log Kcu = 18.6, log KN~ = 16

Cited by 141 publications

References 10 publications

Complexation and Toxicity of Copper in Higher Plants. II. Different Mechanisms for Copper versus Cadmium Detoxification in the Copper-Sensitive Cadmium/Zinc Hyperaccumulator Thlaspi caerulescens (Ganges Ecotype)

Complexation and Toxicity of Copper in Higher Plants. II. Different Mechanisms for Copper versus Cadmium Detoxification in the Copper-Sensitive Cadmium/Zinc Hyperaccumulator Thlaspi caerulescens (Ganges Ecotype)

Iron homeostasis related genes in rice

The Arabidopsis YELLOW STRIPE LIKE4 and 6 Transporters Control Iron Release from the Chloroplast

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