Metal cation toxicity in the alga Gracilaria domingensis as evaluated by the daily growth rates in synthetic seawater

Mendes, Luiz Fernando; Zambotti-Villela, Leonardo; Colepicolo, Pio; Marinho‐Soriano, Eliane; Stevani, Cassius V.; Yokoya, Nair S.

doi:10.1007/s10811-013-0036-1

Cited by 32 publications

(19 citation statements)

References 45 publications

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“…Mendes et al (2013) described this trend after exposure of Gracilaria domingensis to different heavy metal ions, including Cd 2+ (0.006 mg L −1 ). After 7 days of exposure, it was not possible to observe reductions in cell viability in S. cymosum samples treated with Cd.…”

Section: Discussionmentioning

confidence: 92%

“…The algal segments were carefully washed with sterile seawater, incubated with 30 μL of MTT stock solution (0.17 g L −1 MTT, diluted in 1 mL of phosphate-buffered saline (PBS)) in a test tube and sterilized seawater (3-mL final volume) at 37°C for 3 h. Algal fragments were then washed in distilled water, transferred to a test tube containing 1.0 mL of dimethyl sulfoxide (DMSO, Merck, Darmstadt, FRG) (Mendes et al 2013), and specific absorbance was determined in a Tecan microplate spectrophotometer (Infinite M200 PRO, Männedorf, Switzerland) at 570 nm. Cell viability was expressed as per cent of viability relative to 0 mg L −1 Cd at 14 days, which was considered as 100 % of cell viability.…”

Section: Cell Viability Analysismentioning

confidence: 99%

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The brown seaweed Sargassum cymosum: changes in metabolism and cellular organization after long-term exposure to cadmium

et al. 2016

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Sargassum cymosum was exposed to cadmium (Cd) to determine any physiological and ultrastructural effects. To accomplish this, S. cymosum samples were cultivated under photosynthetic active radiation (PAR) and Cd (0, 0.1, 0.2, 0.4 and 0.8 mg L) during 7 and 14 days in laboratory-controlled conditions (0 mg L Cd at both exposure times as control). Seaweeds had high retention capacity (over 90 %) for both exposure times. Growth rates showed significant increases by 14 days, especially for 0.1 and 0.4 mg L Cd. Photosynthetic parameters were unaffected by Cd treatments. Chlorophyll contents were present in higher concentrations for all Cd treatments compared to respective control. Carotenoid profile showed significant differences in total composition and proportion of fucoxanthin and β-carotene, and no lutein was detected at 14 days. Phenolic and flavonoid compounds showed major accumulation at 14 days. Transmission electron microscopy (TEM) analyses presented major alterations in Cd-treated samples, when compared with respective control, in particular disorganization of cell wall fibrils. When compared to respective control samples, multivariate analyses showed disparate and complex interactions among metabolites in Cd-exposed seaweeds, giving evidence of physiological defence response. Thus, it can be concluded that Cd is a stressor for S. cymosum, resulting in physiological and structural alterations related to defence mechanisms against oxidative stress and toxicological effects resulting from long-term metal exposure. However, in the present paper, some observed changes also appear to result from acclimation mechanisms under lower concentration of Cd relative to the tolerance of S. cymosum to experimental conditions.

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Section: Discussionmentioning

confidence: 92%

Section: Cell Viability Analysismentioning

confidence: 99%

The brown seaweed Sargassum cymosum: changes in metabolism and cellular organization after long-term exposure to cadmium

et al. 2016

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“…The effects of monovalent, bivalent, and trivalent metals on the seaweed G. domingensis as evaluated by DGR was previously reported by our group . Table displays the toxicity of monovalent, divalent, and trivalent metal cations as total IC50 values as determined from DGR curves versus metal concentrations . The IC50 data were converted to free ions (IC50F and log IC50F values) by MINTEQA2 as shown in Table .…”

Section: Resultsmentioning

confidence: 99%

“…The result of the last combination, or covalent index (X m 2 r), reflects the cation-binding tendency of soft ligands as sulfur-containing biomolecules [19,21]. Despite the considerable ecological and economic importance of marine seaweeds from the Gracilaria genus to the pharmaceutical industry and as raw materials for agar production [22][23][24][25], metal toxicity studies using the QICAR approach are scarce. For the present study, we modeled the effects of some metal cation physicochemical parameters on their toxicity to a representative species of the red seaweed Gracilaria domingensis (Kütz.)…”

Section: Introductionmentioning

confidence: 99%

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Prediction of mono‐, bi‐, and trivalent metal cation relative toxicity to the seaweed Gracilaria domingensis (Gracilariales, Rhodophyta) in synthetic seawater

Mendes

Zambotti-Villela

Yokoya

et al. 2013

Enviro Toxic and Chemistry

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The present study reports a 48-h aquatic metal-toxicity assay based on daily growth rates of the red seaweed Gracilaria domingensis (Gracilariales, Rhodophyta) in synthetic seawater. The median inhibitory concentration (IC50) for each metal cation was experimentally determined, and the ratios of free ions (aqueous complex) were calculated by software minimization of the total equilibrium activity (MINTEQA2) to determine the free median inhibitory concentration (IC50F). A model for predicting the toxicity of 14 metal cations was developed using the generic function approximation algorithm (GFA) with log IC50F values as the dependent variables and the following properties as independent variables: ionic radius (r), atomic number (AN), electronegativity (Xm ), covalent index (Xm (2) r), first hydrolysis constant (|log KOH |), softness index (σp ), ion charge (Z), ionization potential (ΔIP), electrochemical potential (ΔEo ), atomic number divided by ionization potential (AN/ΔIP), and the cation polarizing power for Z(2) /r and Z/AR. The 3-term independent variables were predicted as the best-fit model (log IC50F: -23.64 + 5.59 Z/AR + 0.99 |log KOH | + 37.05 σp ; adjusted r(2) : 0.88; predicted r(2) : 0.68; Friedman lack-of-fit score: 1.6). This mathematical expression can be used to predict metal-biomolecule interactions, as well as the toxicity of mono-, bi-, and trivalent metal cations, which have not been experimentally tested in seaweed to date. Quantitative ion-character relationships allowed the authors to infer that the mechanism of toxicity might involve an interaction between metals and functional groups of biological species containing sulfur or oxygen.

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The influence of salinity on growth, morphology, leaf ultrastructure, and cell viability of the seagrass Halodule wrightii Ascherson

et al. 2016

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Metal cation toxicity in the alga Gracilaria domingensis as evaluated by the daily growth rates in synthetic seawater

Cited by 32 publications

References 45 publications

The brown seaweed Sargassum cymosum: changes in metabolism and cellular organization after long-term exposure to cadmium

The brown seaweed Sargassum cymosum: changes in metabolism and cellular organization after long-term exposure to cadmium

Prediction of mono‐, bi‐, and trivalent metal cation relative toxicity to the seaweed Gracilaria domingensis (Gracilariales, Rhodophyta) in synthetic seawater

The influence of salinity on growth, morphology, leaf ultrastructure, and cell viability of the seagrass Halodule wrightii Ascherson

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