Metal binding properties of Klebsiella oxytoca

Wallberg, Malin; Brynhildsen, Lena; Allard, Bert

doi:10.1007/bf00282921

Cited by 8 publications

(5 citation statements)

References 14 publications

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“…The apparent metal affinity order of Cs<Sr,Zn,Cd<Eu<Hg (cf. Figure 1) was confirmed by a Duncan multiple-range test, and this result agrees with previous studies of various bacteria and isolated cell walls (Marquis et al, 1976;Mullen et al, 1989;Wallberg et al, 1991). About 54% of the total variance within the data base was found to be simply effects of the various elements.…”

Section: Discussionsupporting

confidence: 91%

“…Consequently, the importance of bacterial metal accumulation under typical environmental conditions is poorly understood. The importance of pH for bacterial metal accumulation has been demonstrated (Pedersen and Albinsson, 1991;Premuzic et al, 1991;Wallberg et al, 1991). On the other hand, there is very little, if any, data concerning the influence of ionic strength, particularly the combined effects of pH and ionic strength on bacterial metal adsorption.…”

Section: Introductionmentioning

confidence: 94%

“…Metal distribution studies were performed in KC1 solution at two ionic strengths (0.01 and 0.1 M), using a radiotracer batch distribution technique (Wallberg et al, 1991). The nuclides used were 134Cs, 85Sr, 152Eu, 65Zn, 1~…”

Section: Metal Adsorption Studiesmentioning

confidence: 99%

“…The culture was harvested at late log phase, washed and starved in 0.1 or 0.01 M KC1 as previously described (Wallberg et al, 1991). Cell density was determined from the optical density (A550) correlated to dry weight and viable counts.…”

Section: Bacteria and Growth Conditionsmentioning

confidence: 99%

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Effects of pH and ionic strength on the adsorption of Cs, Sr, Eu, Zn, Cd and Hg byPseudomonas putida

Ledin

Pedersen

Allard

1997

Water Air Soil Pollut

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Abstract. Bacterial metal accumulation may influence the mobility and chemical form (speciation) of metals in the environment. The passive adsorption of six metals (Cs, St, Eu, Zn, Cd and Hg) by a soil bacterium, Pseudomonas puti&~, was studied in the present work. using a radiotracer batchdistribution technique. To replicate natural conditions, the adsorption was considered as a function of pH (4-10) and ionic strength (0.01 M and 0.1 M KCI) at a low metal concentration ( 10-s M). E putida exhibited a total metal accumulating capacity of 200-1000 meq kg-t bacteria (dry weight) (measured m 0.01 M KCI at pH 6.4). This capacity is comparable to that of many organic soil components and it is above the capacity of most inorganic constituents. The lk~llowing affinity order of adsorption was observed: Hg>Eu>Cd,Zn,Sr>Cs. The results indicate that the bacterial surface carries different sites that exhibit varying affinity and capacity for binding metal ions. It can be concluded that the overall adsorption of metals by/3. putida is determined by several interacting processes related to the properties of both the metals and the bacterial surface and to the composition of the solution phase (pH as well as ionic strength).

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 94%

Section: Metal Adsorption Studiesmentioning

confidence: 99%

Section: Bacteria and Growth Conditionsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of pH and ionic strength on the adsorption of Cs, Sr, Eu, Zn, Cd and Hg byPseudomonas putida

Ledin

Pedersen

Allard

1997

Water Air Soil Pollut

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“…An enhanced adsorption at higher pH could be due to an increase in the negative charge of surface functional groups and also partly to the formation of hydroxide and carbonate complexes with the metals, complexes that could precipitate within (Geisweid & Urbach 1983, Norberg & Persson 1984 or become associated with the cell walls. However, the accumulation of zinc by K. oxytoca in 0.1 M KC1 was maximum at around pH 6 (Wallberg et al 1991), which is similar to the pH dependence of C. johnsonae in CJ/10 medium (see below).…”

Section: Discussionsupporting

confidence: 50%

Accumulation of zinc and cadmium by Cytophaga johnsonae

et al. 1996

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Passive and active accumulation of zinc and cadmium by a common soil and freshwater bacterium, Cytophaga johnsonae, was studied using a radio-tracer batch distribution technique. The effects of variation of pH (3-10), as well as of ionic strength (0.007 and 0.07 M) on passive accumulation of the metals were examined. For both zinc and cadmium, accumulation was mainly due to passive processes, such as surface adsorption and/or diffusion into the periplasm. However, at low zinc concentrations, accumulation increased when glucose was added, suggesting an active uptake; at higher zinc concentrations such uptake was not detected, probably because it was masked by the stronger sorption properties of the cell wall. Adsorption of the metals was pH dependent: at higher ionic strength, accumulation was enhanced at pH values above 7; at lower ionic strength, adsorption differed and was markedly higher, with increased accumulation between pH 5 and 8.

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Interactions between a soil fungus, Trichoderma harzianum, and IIb metals?adsorption to mycelium and production of complexing metabolites

1993

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Fungi are capable of accumulating metals and, in soil, such accumulation may influence metal speciation and transport. The interactions between a common soil fungus, Trichoderma harzianum, and IIb elements were studied in the present investigation. The accumulation of the metals zinc, cadmium and mercury by starved and non-starved mycelium at different pH was determined by a batch technique using radioactive tracers; uptake of the metals was found to be large, with respective distribution coefficients of about 103"5, 10 z'5 and 104.0 for zinc, cadmium and mercury, respectively. Metal accumulation by a starved system was largely independent of pH in the range 3-9, where in a non-starved system an increased accumulation of zinc (at 10 -8 M) was observed at low pH (3-5). Potentiometric titrations performed on the two systems revealed significant differences in acid capacities, i.e. values close to zero for the starved system and 500-800 meq kg -1 for the non-starved system. The maximum metal uptake was at least 50 mmol kg -1 at pH 6.5 (calculated from adsorption isotherms). The present findings suggests that in the non-starved system a metabolite is produced and then released when the pH is within a certain range.

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Metal binding properties of Klebsiella oxytoca

Cited by 8 publications

References 14 publications

Effects of pH and ionic strength on the adsorption of Cs, Sr, Eu, Zn, Cd and Hg byPseudomonas putida

Effects of pH and ionic strength on the adsorption of Cs, Sr, Eu, Zn, Cd and Hg byPseudomonas putida

Accumulation of zinc and cadmium by Cytophaga johnsonae

Interactions between a soil fungus, Trichoderma harzianum, and IIb metals?adsorption to mycelium and production of complexing metabolites

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