Metal binding ability of microbial natural metal chelators and potential applications

Hofmann, Marika; Retamal-Morales, Gerardo; Tischler, Dirk

doi:10.1039/c9np00058e

Cited by 58 publications

(30 citation statements)

References 216 publications

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“…Siderophores (greek: sidero = iron; phore = carrier) are secondary metabolites produced by many organisms (plant, bacteria, and fungi) to sequester iron in a physiological context (Kem and Butler 2015;Carroll and Moore 2018;Hofmann et al 2020). Often stress, such as iron limitation or ecological pressure, leads to a response of producing and secreting secondary metabolites.…”

Section: Nmos Involved In Siderophore Biosynthesismentioning

confidence: 99%

“…The diverse nature of siderophores and their structural as well as functional elements provide access towards many applications, as has been extensively reviewed (Saha et al 2016;Su et al 2018;Albelda-Berenguer et al 2019;Řezanka et al 2019;Hofmann et al 2020) and will not be discussed in detail here. a b c Fig.…”

Section: Nmos Involved In Siderophore Biosynthesismentioning

confidence: 99%

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Flavin-dependent N-hydroxylating enzymes: distribution and application

Mügge

Heine

Baraibar

et al. 2020

Appl Microbiol Biotechnol

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Amino groups derived from naturally abundant amino acids or (di)amines can be used as "shuttles" in nature for oxygen transfer to provide intermediates or products comprising NO functional groups such as N-hydroxy, oxazine, isoxazolidine, nitro, nitrone, oxime, C-, S-, or N-nitroso, and azoxy units. To this end, molecular oxygen is activated by flavin, heme, or metal cofactorcontaining enzymes and transferred to initially obtain N-hydroxy compounds, which can be further functionalized. In this review, we focus on flavin-dependent N-hydroxylating enzymes, which play a major role in the production of secondary metabolites, such as siderophores or antimicrobial agents. Flavoprotein monooxygenases of higher organisms (among others, in humans) can interact with nitrogen-bearing secondary metabolites or are relevant with respect to detoxification metabolism and are thus of importance to understand potential medical applications. Many enzymes that catalyze N-hydroxylation reactions have specific substrate scopes and others are rather relaxed. The subsequent conversion towards various NO or N-N comprising molecules is also described. Overall, flavin-dependent N-hydroxylating enzymes can accept amines, diamines, amino acids, amino sugars, and amino aromatic compounds and thus provide access to versatile families of compounds containing the NO motif. Natural roles as well as synthetic applications are highlighted. Key points • NO and N-N comprising natural and (semi)synthetic products are highlighted. • Flavin-based NMOs with respect to mechanism, structure, and phylogeny are reviewed. • Applications in natural product formation and synthetic approaches are provided.

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Section: Nmos Involved In Siderophore Biosynthesismentioning

confidence: 99%

Section: Nmos Involved In Siderophore Biosynthesismentioning

confidence: 99%

Flavin-dependent N-hydroxylating enzymes: distribution and application

Mügge

Heine

Baraibar

et al. 2020

Appl Microbiol Biotechnol

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“…Extracellular metal sequestration and intracellular metal accumulation also provide resistance for microorganisms dealing with high metal concentrations. Extracellular sequestration involves the immobilization of metal ions outside of the cell by interaction with metal chelators [18] or extracellular polymeric substances (EPS) [1]. EPS adsorb toxic metals directly or facilitate reactions involving the products of energy-related metabolisms (e.g., sulfate reduction produces H 2 S to form metal sulfides, or Fe(II) oxidation produces Fe(III) minerals that sorb or incorporate metals) [1].…”

Section: Introductionmentioning

confidence: 99%

Metagenomic and Metatranscriptomic Study of Microbial Metal Resistance in an Acidic Pit Lake

et al. 2020

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Cueva de la Mora (CM) is an acidic, meromictic pit lake in the Iberian Pyrite Belt characterized by extremely high metal(loid) concentrations and strong gradients in oxygen, metal, and nutrient concentrations. We hypothesized that geochemical variations with depth would result in differences in community composition and in metal resistance strategies among active microbial populations. We also hypothesized that metal resistance gene (MRG) expression would correlate with toxicity levels for dissolved metal species in the lake. Water samples were collected in the upper oxic layer, chemocline, and deep anoxic layer of the lake for shotgun metagenomic and metatranscriptomic sequencing. Metagenomic analyses revealed dramatic differences in the composition of the microbial communities with depth, consistent with changing geochemistry. Based on relative abundance of taxa identified in each metagenome, Eukaryotes (predominantly Coccomyxa) dominated the upper layer, while Archaea (predominantly Thermoplasmatales) dominated the deep layer, and a combination of Bacteria and Eukaryotes were abundant at the chemocline. We compared metal resistance across communities using a curated list of protein-coding MRGs with KEGG Orthology identifiers (KOs) and found that there were broad differences in the metal resistance strategies (e.g., intracellular metal accumulation) expressed by Eukaryotes, Bacteria, and Archaea. Although normalized abundances of MRG and MRG expression were generally higher in the deep layer, expression of metal-specific genes was not strongly related to variations in specific metal concentrations, especially for Cu and As. We also compared MRG potential and expression in metagenome assembled genomes (MAGs) from the deep layer, where metal concentrations are highest. Consistent with previous work showing differences in metal resistance mechanisms even at the strain level, MRG expression patterns varied strongly among MAG populations from the same depth. Some MAG populations expressed very few MRG known to date, suggesting that novel metal resistance strategies remain to be discovered in uncultivated acidophiles.

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“…This study also revealed that both acinetobactins are versatile metal chelators ( Fig. 4 ), not specific to iron, and the physiological relevance of this property was also of interest ( 53 ). However, ICP-OES/MS-based metal sequestration assays with calprotectin ( Fig.…”

Section: Discussionmentioning

confidence: 70%

Distinctive Roles of Two Acinetobactin Isomers in Challenging Host Nutritional Immunity

Kim

Song

et al. 2021

mBio

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Acinetobacter baumannii has acquired antibiotic resistance at an alarming rate, and it is becoming a serious threat to society, particularly due to the paucity of effective treatment options. Acinetobactin is a siderophore of Acinetobacter baumannii , responsible for active iron supply, and it serves as a key virulence factor to counter host nutritional immunity during infection.

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Metal binding ability of microbial natural metal chelators and potential applications

Abstract: Metallophores can chelate many different metal and metalloid ions next to iron, make them valuable for many applications.

Cited by 58 publications

References 216 publications

Flavin-dependent N-hydroxylating enzymes: distribution and application

Flavin-dependent N-hydroxylating enzymes: distribution and application

Metagenomic and Metatranscriptomic Study of Microbial Metal Resistance in an Acidic Pit Lake

Distinctive Roles of Two Acinetobactin Isomers in Challenging Host Nutritional Immunity

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