Metal-Based Biologically Active Compounds: Synthesis, Characterization, DNA Interaction, Antibacterial, Cytotoxic and SOD Mimic Activities

Abstract

The square pyramidal copper(II) complexes of N, O- donor ligand and ciprofloxacin have been synthesized. Synthesized complexes were characterized by physicochemical parameters like elemental analysis, electronic, FT-IR and LC-MS spectra. The complexes were screened for their antimicrobial activity against Gram(+Ve), i.e. Staphylococcus aureus, Bacillus subtilis, and Gram(-Ve), i.e. Serratia marcescens, Pseudomonas aeruginosa and Escherichia coli, microorganisms in terms of minimum inhibitory concentration and colony-forming unit. To determine the binding mode of complexes with Herring Sperm DNA, absorption titration and viscosity measurement were employed. DNA cleavage activity was carried out by gel electrophoresis experiment using supercoiled form of pUC19 DNA. The complexes were tested for their superoxide dismutase mimic activity in terms of IC(50) value. Synthesized complexes were also screened for their cytotoxicity using brine shrimp lethality assay method.

Synthesis, structure and superoxide dismutase-like activity of copper(II) complexes based on N,N′-bis(2-quinolinylmethyl)amantadine

“…The peak at 1593 cm À1 is ascribed to the C@N breathing vibration of the quinoline group, which is shifted towards lower frequencies of 1599 and 1598 cm À1 , respectively in complexes 1 and 2. Some new brands with medium to weak intensities appear in the region 498-530 cm À1 , which are assigned to Cu-N stretching vibrations [31]. This fact is agreement with the results determined by X-ray diffraction.…”

Synthesis and Characterization with Antimicrobial Activity Studies on some Transition Metal Complexes of N, O Donor Novel Schiff Base Ligand

“…Many drugs possess modified toxicological and pharmacological properties in the form of metal complex and probably Schiff bases are versatile C=N (imine) containing compounds possessing broad spectrum of biological activity and incorporation of metals in the form of complexes showed some degree of antibacterial, antifungal, antitumor, anticancer and anti-inflammatory activity [4][5][6][7][8]. In vivo studies have indicated that some biologically active compounds may become more carcinostatic and bacteriostatic upon chelation [9][10][11][12][13]. Such interaction with transition metal ions with amino acids, peptides and nucleic acids, are of immense biological importance [14][15][16].…”

Spectral, thermal, molecular modeling and biological studies on mono- and binuclear complexes derived from oxalo bis(2,3-butanedionehydrazone)

“…The coordination sites are two azomethine nitrogens of the hydrazone moiety and two carbonyl groups of amidic moiety; each two donors chelated one metal ion. The shift of υ(C=N) to 1542 cm −1 and υ(C=O) amidic to 1644 in the Pd(II) complex and to 1552 and 1676 in the Ni(II) complex together with appearance of υ(M–N) [22] and υ(M–O) bands at ~465 and ~540 cm −1 , respectively. In the Ni(II) complex, the band of carbonyl groups splitted to two at 1697 and 1676 cm −1 ; the first is due to ketonic group which is not participated in bonding.…”