Metagenomics revealing molecular profiling of microbial community structure and metabolic capacity in Bamucuo, Tibet

Peng, Syd S.; Wei, Cai; Sun, Dan; Yuan, Wenliang; Li, Lei; Dai, Chaoxu; Chen, Zuozhou; Zeng, Xiaomin; Wang, Shihang; Zhang, Yuyang; Jiang, Shouwen; Wu, Zhichao; Liu, Dong; Jiang, Linhua

doi:10.1101/2022.01.18.476867

Cited by 2 publications

(2 citation statements)

References 98 publications

(145 reference statements)

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“…Investigating the change in bacterial communities along the altitudinal gradient will shed light on the prediction of future climatic changes scenario (Yuan et al, 2014 ). Wei et al ( 2022 ) characterized the microbial community composition and metabolic capacity in Qinghai-Tibet Plateau high-altitude areas (with an altitude of 4,555 m) and confirmed that Qinghai-Tibet Plateau is a huge and valuable resource bank in which more new non-resistant antibiotics and many other bioactive substances could be developed. Characterizing the microbial community composition is important for forage preservation because phyllosphere microbial flora play a critical role in ensiling fermentation and provide sufficient fodder for local livestock during winter and spring.…”

Section: Introductionmentioning

confidence: 96%

Altitudinal Distribution Patterns of Phyllosphere Microbial Communities and Their Contribution to Silage Fermentation of Kobresia pygmaea Along the Elevation Gradient on the Tibetan Plateau

et al. 2022

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The study aimed to reveal altitudinal distribution patterns of phyllosphere microbial communities and silage fermentation of Kobresia pygmaea along the elevation gradient on the Tibetan Plateau. The K. pygmaea was individually collected from 2,500, 3,000, 4,000, 4,500, and 5,000 m above sea level (a.s.l.) on the Tibetan Plateau and ensiled for 60 days, respectively. The phyllosphere bacterial diversity increased while fungal diversity decreased along the elevation gradient, and bacterial and fungal richness showed a unimodal distribution with peak abundance at 4,000 and 3,000 m a.s.l., respectively. After 60 days of ensiling, the bacterial and fungal community composition changed but did not exhibit clear altitudinal distribution patterns. All K. pygmaea underwent a weak fermentation indicated by pH above 5.0 and low ratio of lactic/acetic acid (LA/AA). The S5000 and S3000 showed the highest and lowest pH, respectively. Although Lactobacillus dominated S4000 after 60 days of ensiling, S4000 still exhibited poor fermentation quality as well as silages from the other four regions. The higher ammonia N concentrations in S3000 and S4000 than the other silages were consistent with the detectable butyric acid in S3000 and S4000. The silage fermentation of K. pygmaea collected from five regions exhibited poor fermentation quality, thereby inoculating lactic acid bacteria to K. pygmaea before ensiling is highly recommended to improve fermentation quality on the Tibetan Plateau.

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Section: Introductionmentioning

confidence: 96%

Altitudinal Distribution Patterns of Phyllosphere Microbial Communities and Their Contribution to Silage Fermentation of Kobresia pygmaea Along the Elevation Gradient on the Tibetan Plateau

et al. 2022

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“…Recently, one study reported only 20 MAGs from soil samples (>4500 a.s.l.) in the Tibetan Alpine Grassland (Wei et al, 2022). In our study, 96 non-redundant MAGs were obtained from all samples.…”

Section: Discussionmentioning

confidence: 99%

Microbially enhanced methane uptake under warming enlarges ecosystem carbon sink in a Tibetan alpine grassland

Zhao

Tian

et al. 2022

Global Change Biology

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The alpine grasslands of the Tibetan Plateau store 23.2 Pg soil organic carbon, which becomes susceptible to microbial degradation with climate warming. However, accurate prediction of how the soil carbon stock changes under future climate warming is hampered by our limited understanding of belowground complex microbial communities. Here, we show that 4 years of warming strongly stimulated methane (CH 4 ) uptake by 93.8% and aerobic respiration (CO 2 ) by 11.3% in the soils of alpine grassland ecosystem. Due to no significant effects of warming on net ecosystem CO 2 exchange (NEE), the warming-stimulated CH 4 uptake enlarged the carbon sink capacity of whole ecosystem. Furthermore, precipitation alternation did not alter such warming effects, despite the significant effects of precipitation on NEE and soil CH 4 fluxes were observed. Metagenomic sequencing revealed that warming led to significant shifts in the overall microbial community structure and the abundances of functional genes, which contrasted to no detectable changes after 2 years of warming. Carbohydrate utilization genes were significantly increased by warming, corresponding with significant increases in soil aerobic respiration. Increased methanotrophic genes and decreased methanogenic genes were observed under warming, which significantly (R 2 = .59, p < .001) correlated with warming-enhanced CH 4 uptakes. Furthermore, 212 metagenome-assembled genomes were recovered, including many populations involved in the degradation of various organic matter and a highly abundant methylotrophic population of the Methyloceanibacter genus. Collectively, our results provide compelling evidence that specific microbial functional traits for CH 4 and CO 2 cycling | 6907 QI et al.

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Metagenomics revealing molecular profiling of microbial community structure and metabolic capacity in Bamucuo, Tibet

Cited by 2 publications

References 98 publications

Altitudinal Distribution Patterns of Phyllosphere Microbial Communities and Their Contribution to Silage Fermentation of Kobresia pygmaea Along the Elevation Gradient on the Tibetan Plateau

Altitudinal Distribution Patterns of Phyllosphere Microbial Communities and Their Contribution to Silage Fermentation of Kobresia pygmaea Along the Elevation Gradient on the Tibetan Plateau

Microbially enhanced methane uptake under warming enlarges ecosystem carbon sink in a Tibetan alpine grassland

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