Metagenomics revealed the phase-related characteristics during rapid development of halotolerant aerobic granular sludge

Li, Wei; Yao, Jin-chi; Zhuang, Jin-Long; Zhou, Yuanyuan; Shapleigh, James P.; Liu, Yongdi

doi:10.1016/j.envint.2020.105548

Cited by 26 publications

(3 citation statements)

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“…The optimal NaCl concentration and temperature for growth of Mangrovibacter were 1% and 30°C, respectively [39]. Li et al [40] found that Mangrovibacter was the dominant bacteria in halotolerant aerobic granular sludge for treating saline wastewater with a salinity of 3%. In addition, the abundances of Enterococcus, Clostridiisalibacter and Weissella were signi cantly higher in the groups with 70 g/L NaCl (Fig.…”

Section: Effect Of Nacl Concentration On Microbial Composition Differ...mentioning

confidence: 99%

Can Salt-Tolerant Sludge Mitigate the Salt Inhibition to Acidogenic Fermentation of Food Waste? Insight Into Volatile Fatty Acid Production and Microbial Community

Yin

Chen

2022

Waste Biomass Valor

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For treatment of saline wastewater, the feasible approach to mitigate the salt inhibition is using the acclimated salt-tolerant sludge. The aim of this work was to verify if the use of the acclimated sludge (AS) also could alleviate salinity stress on acidogenic fermentation of food waste (FW) under saline environment. The responses of volatile fatty acid (VFA) production and the microbial community to salt stress were investigated. Results showed that VFA production was reduced by high salinity (30 g/L and 70 g/L NaCl) compared with the control (0 g/L NaCl), especially for groups inoculated with the AS, whereas inoculating with the non-acclimated sludge (non-AS) caused less reduction. The impact of salinity was seen on VFA production with accumulation of more propionic acid and acetic acid along with traces of butyric acid. Signi cant shift on microbial community composition occurred upon biomass exposure to salt. The microbial communities of the non-AS and AS groups at the same NaCl concentrations converged over time. The non-AS groups contained a more proportion of the phyla Bacteroidetes, Atribacteria and Chloro exi at high salt levels. These ndings demonstrate that the non-AS was more conducive to VFA production due to the presence of higher proportions of hydrolytic and fermenting bacteria. Statement of NoveltyAlthough anaerobic digestion (AD) would be the most cost-effective and sustainable technology, the salinity is considered to be inhibitory to anaerobic biological treatment processes. The recent applications of salt-tolerant cultures for the treatment of saline wastewaters suggest that biological treatment is promising. Previous studies also reported that acidogenic fermentation as the rst step of AD process is inhibited under saline conditions. However, no study to date has focused on acidogenic fermentation for volatile fatty acid production from food waste using salt-tolerant sludge. Therefore, there is a need for improved understanding of high salt stress to resource recovery from organic wastes. This understanding can help in the design of an operating strategy to alleviate the inhibition of waste treatment by salinity.

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Section: Effect Of Nacl Concentration On Microbial Composition Differ...mentioning

confidence: 99%

Can Salt-Tolerant Sludge Mitigate the Salt Inhibition to Acidogenic Fermentation of Food Waste? Insight Into Volatile Fatty Acid Production and Microbial Community

Yin

Chen

2022

Waste Biomass Valor

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“…The primary sources of MGB pure cultures were predominantly obtained from the regions of East Asia, South East Asia, and South Asia and extensively identified in various habitats ( Table 1 , Supplementary Tables S1A , S1B ) using 16S rRNA-based amplicon sequencing analysis. The distribution of MGBs includes high-salt sludges ( Li et al, 2019 , 2020 ; Yin et al, 2022 ), tannery effluent ( Lian et al, 2016a , b ; Sanjay et al, 2020 ), brackish water ( Kiun et al, 2016 ), mangrove rhizosphere sediment (BioProject no PRJNA682484), farm soil ( Zhang et al, 2015 ), shrimp aquaculture farm ( Joseph et al, 2014 ), storage tanks containing biodiesel ( White et al, 2011 ), water droplets from within the bitumen ( Himmelberg, 2019 ), geothermal lake (MYI strain CEMTC_1,393, GenBank accession no. OP143686.1), and pond (“Shoubuike”) ( Fujita et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, in the case of shrimp, MGBs constituted approximately 5% of its total microbiota in the intestine ( Sun and Xu, 2021 ). A significant prevalence of MGBs was observed in high-salt sludge ( Yin et al, 2022 ), aerobic granular sludge (AGS) with the introduction of acyl-homoserine lactone ( Li et al, 2019 ), and at the early phase of halotolerant aerobic granulation for AGS formation ( Li et al, 2020 ). The MGBs account for 45.7, 35.9, and 74.9% of the overall microbiome abundance observed in sludges as mentioned earlier ( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation, molecular identification, and genomic analysis of Mangrovibacter phragmitis strain ASIOC01 from activated sludge harboring the bioremediation prowess of glycerol and organic pollutants in high-salinity

Chin,

Ravi Varadharajulu,

Lin

et al. 2024

Front. Microbiol.

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The physiological and genotypic characteristics of Mangrovibacter (MGB) remain largely unexplored, including their distribution and abundance within ecosystems. M. phragmitis (MPH) ASIOC01 was successfully isolated from activated sludge (AS), which was pre-enriched by adding 1,3-dichloro-2-propanol and 3-chloro-1,2-propanediol as carbon sources. The new isolate, MPH ASIOC01, exhibited resilience in a medium containing sodium chloride concentration up to 11% (with optimal growth observed at 3%) and effectively utilizing glycerol as their sole carbon source. However, species delimitation of MGBs remains challenging due to high 16S rRNA sequence similarity (greater than 99% ANI) among different MGBs. In contrast, among the housekeeping gene discrepancies, the tryptophan synthase beta chain gene can serve as a robust marker for fast species delimitation among MGBs. Furthermore, the complete genome of MPH ASIOC01 was fully sequenced and circlized as a single contig using the PacBio HiFi sequencing method. Comparative genomics revealed genes potentially associated with various phenotypic features of MGBs, such as nitrogen-fixing, phosphate-solubilizing, cellulose-digesting, Cr-reducing, and salt tolerance. Computational analysis suggested that MPH ASIOC01 may have undergone horizontal gene transfer events, possibly contributing unique traits such as antibiotic resistance. Finally, our findings also disclosed that the introduction of MPH ASIOC01 into AS can assist in the remediation of wastewater chemical oxygen demand, which was evaluated using gas chromatograph-mass spectrometry. To the best of our knowledge, this study offers the most comprehensive understanding of the phenotypic and genotypic features of MGBs to date.

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Humibacter

Carro¹

2023

Bergey's Manual of Systematics of Archaea and Bacteria

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Hu.mi.bac'ter. L. masc. n. humus , earth, soil and, in earth sciences or agriculture, humus; N.L. masc. n. bacter , rod; N.L. masc. n. Humibacter , rod living in the humus. Actinobacteriota / Actinomycetia / Actinomycetales / Microbacteriaceae / Humibacter Cells are Gram‐stain‐positive rods (0.3–0.7 × 0.8–1.8 μm), non‐spore‐forming, motile, or nonmotile. Mesophilic and strictly aerobic chemo‐organotroph. Strains have been isolated from different types of soils, wood chips, and sewage sludge compost samples. In addition, one strain was obtained from a small intestine of castrated beef cattle. Produce acid phosphatase, leucine arylamidase, α‐glucosidase, and β‐glucosidase enzymes but not α‐chymotrypsin. The peptidoglycan contains 2,4‐diaminobutyric acid as diagnostic diamino acid and several strains also include ornithine. The major quinones are the menaquinones MK‐11 and MK‐12; in addition, MK‐10 can be present. The characteristic polar lipid is phosphatidylglycerol, while diphosphatidylglycerol and several unidentified phospholipids, glycolipids, aminolipids, and lipids could be present. Phylogenetically, a member of the family Microbacteriaceae . DNA G + C content (mol%) : 62–68 (HPLC, WGS). Type species : Humibacter albus Vaz‐Moreira et al. 2008a VP .

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Metagenomics revealed the phase-related characteristics during rapid development of halotolerant aerobic granular sludge

Cited by 26 publications

References 53 publications

Can Salt-Tolerant Sludge Mitigate the Salt Inhibition to Acidogenic Fermentation of Food Waste? Insight Into Volatile Fatty Acid Production and Microbial Community

Can Salt-Tolerant Sludge Mitigate the Salt Inhibition to Acidogenic Fermentation of Food Waste? Insight Into Volatile Fatty Acid Production and Microbial Community

Isolation, molecular identification, and genomic analysis of Mangrovibacter phragmitis strain ASIOC01 from activated sludge harboring the bioremediation prowess of glycerol and organic pollutants in high-salinity

Humibacter

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