Metagenomics data of microbial communities of natural organic matter from the dispersion train of sulfide tailings

Abstract

Below is data on the microbial diversity of natural organic matter from the Dispersion Train of Sulfide Tailings (northern Salaire Ridge, southwestern Siberia, Russia, Ursk Village). Data was obtained using 16s rRNA amplicon directed metagenomic sequencing on Illumina MiSeq. The raw sequence data used for analysis is available in NCBI under the Sequence Read Archive (SRA) with BioProject No. PRJNA670045 and SRA accession number SRX9314152, SRX9314376. The data sequences of the 16s rRNA gene are presented at the links MW142408-MW142413, MW142414-MW142447.

Metagenomics data of microbial communities in bacterial mats and bottom sediments in water bodies within the Kurai Mercury Province (Gorny Altai, Russia)

“…We used the degenerate primers U806R (5′-GGACTACNVGGGTWTCTAAT-3′) and U343F (5′-CCTACGGGRSGCAGCAG-3′) to obtain the target 16S rRNA fragment (region V3-V4). The primers used have previously proven their ability to amplify a wide range of microorganisms from various phyla [7] , [8] , [9] . To obtain a library of gene fragments with the smallest number shift, we used low-temperature annealing of primers and Fusion Polymerase Q5 from New England Biolabs.…”