Metagenomics-Based Analysis of the Age-Related Cumulative Effect of Antibiotic Resistance Genes in Gut Microbiota

Wu, Lei; Xie, Xinqiang; Liu, Ying; Liang, Tingting; Zhong, Hao‐Jie; Jun, Ma; Yang, Lingshuang; Yang, Juan; Li, Longyan; Yu, Xiaoyan; Li, Haixin; Zhang, Jumei; Chen, Xuefeng; Ding, Yu; Wu, Qingping

doi:10.3390/antibiotics10081006

Cited by 22 publications

(21 citation statements)

References 88 publications

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“…This is the first large-scale retrospective trial of abnormal blood glucose metabolism in China, including high blood glucose group and normal blood glucose group. The clinical evidence of the effect of WMT on blood glucose metabolism has been established, which lays the foundation for subsequent studies on the effects of gut microbiota (53), metabolic markers (54), and diet (55) regulation on abnormal blood glucose metabolism. Taken together, these results suggest that restoring gut microbiota may serve as a promising treatment for abnormal blood glucose metabolism; however, the mechanism of action requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Washed microbiota transplantation improves patients with high blood glucose in South China

Xie

et al. 2022

Front. Endocrinol.

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Background and AimsAlthough fecal microbiota transplantation (FMT) from healthy donors has been shown to have hypoglycemic effects in animal models of diabetes, its clinical impact in patients with abnormal blood glucose metabolism is unclear, especially in southern Chinese populations. The aim of this study was to investigate the feasibility and efficacy of washed microbiota transplantation (WMT) in the treatment of abnormal blood glucose metabolism in a population in southern China.MethodsThe clinical data of patients with different indications who received 1-3 treatments of WMT were retrospectively collected. The changes of blood glucose, blood lipids, blood pressure, liver function and blood routine before and after WMT were compared, such as fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG), systolic blood pressure (SBP), white blood cells (WBC), lymphocytes (LY) and platelets (PLT), etc.ResultsA total of 195 patients were included in the First Affiliated Hospital of Guangdong Pharmaceutical University, including 20 patients with high blood glucose and 175 patients with normal blood glucose. WMT has a significant effect in reducing short term blood glucose level (FBG) in patients with high blood glucose (p < 0.05). The fasting blood glucose (FBG) of 72.22% of patients with high blood glucose decreased to normal in a short term (about 1 month) (p < 0.001); In the medium term (about 2 months), there was a significant hypolipidemic (TG) (p = 0.043) effect, long term (about 6 months) significant blood pressure lowering (SBP, p = 0.048) effect. Overall, WMT significantly reduced the risk of high risk classes of Atherosclerotic Cardiovascular Disease (ASCVD) in the short term (p = 0.029) and medium term (p = 0.050).ConclusionWMT can significantly improve blood glucose in patients with high blood glucose, and there is no long-term elevated risk of blood glucose and ASCVD. FBG levels were significantly reduced in both the short and medium term in patients with high blood glucose treated with WMT. Therefore, the regulation of gut microbiota by WMT may provide a new clinical approach for the treatment of abnormal blood glucose metabolism.

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Section: Discussionmentioning

confidence: 99%

Washed microbiota transplantation improves patients with high blood glucose in South China

Xie

et al. 2022

Front. Endocrinol.

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“…This is a large-scale retrospective trial of MS in southern China, including a MS group and a non-MS group. The clinical evidence of the effect of WMT on MS has been established, laying a foundation for the follow-up study of the effects of gut microbiota ( Wu et al., 2021b ) and metabolic markers ( Wu et al., 2022b ) on metabolic abnormalities. Taken together, these results suggest that restoring gut microbiota may serve as a promising treatment for MS; however, the mechanism of action requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Washed microbiota transplantation improves patients with metabolic syndrome in South China

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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BackgroundMetabolic syndrome (MS) is a growing public health problem worldwide. The clinical impact of fecal microbiota transplantation (FMT) from healthy donors in MS patients is unclear, especially in southern Chinese populations. This study aimed to investigate the effect of washed microbiota transplantation (WMT) in MS patients in southern China.MethodsThe clinical data of patients with different indications receiving 1-3 courses of WMT were retrospectively collected. The changes of BMI, blood glucose, blood lipids, blood pressure and other indicators before and after WMT were compared, such as fasting blood glucose (FBG), glycated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c)), high-density lipoprotein cholesterol (HDL-c), non-high-density lipoprotein (non-HDL-c), systolic blood pressure (SBP), diastolic blood pressure (DBP), etc. At the same time, comprehensive efficacy evaluation and atherosclerotic cardiovascular disease (ASCVD) grade assessment were performed on MS patients. Finally, 16S rRNA gene amplicon sequencing was performed on fecal samples of MS patients before and after transplantation.ResultsA total of 237 patients were included, including 42 in the MS group and 195 in the non-MS group. For MS patients, WMT significantly improved the comprehensive efficacy of MS in short term 40.48% (p<0.001), medium term 36.00% (p=0.003), and long term 46.15% (p=0.020). Short-term significantly reduced FBG (p=0.023), TG (p=0.030), SBP (p=0.026) and BMI (p=0.031), and increased HDL-c (p=0.036). The medium term had a significant reduction in FBG (p=0.048), TC (p=0.022), LDL-c (p=0.043), non-HDL-c (p=0.024) and BMI (p=0.048). WMT had a significant short term (p=0.029) and medium term (p=0.011) ASCVD downgrading effect in the high-risk group of MS patients. WMT improved gut microbiota in MS patients.ConclusionWMT had a significant improvement effect on MS patients and a significant downgrade effect on ASCVD risk in the high-risk group of patients with MS. WMT could restore gut microbiota homeostasis in MS patients. Therefore, the regulation of gut microbiota by WMT may provide a new clinical approach for the treatment of MS.

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“…This was A. indonesiensis as well, but showed in vitro activity against aminoglycosides, tetracyclines, imipenem, and ceftriaxone [ 10 ]. Recently, Wu et al [ 11 ] identified, in a metagenomic approach, a genus Acetobacter in human fecal samples as one of the main genera carrying the top 20 antibiotic resistance gene types. So far, there are no data on the AMR of Komagataeibacter species.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance of Acetobacter and Komagataeibacter Species Originating from Vinegars

Cepec

Trček

2022

IJERPH

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Consumers’ preference towards healthy and novel foods dictates the production of organic unfiltered bottled vinegar that still contains acetic acid bacteria. After ingesting vinegar, the bacteria come into close contact with the human microbiota, creating the possibility of horizontal gene transfer, including genetic determinants for antibiotic resistance. Due to the global spread of antimicrobial resistance (AMR), we analyzed the AMR of Acetobacter and Komagataeibacter species originating mainly from vinegars. Six antibiotics from different structural groups and mechanisms of action were selected for testing. The AMR was assessed with the disk diffusion method using various growth media. Although the number of resistant strains differed among the growth media, 97.4%, 74.4%, 56.4%, and 33.3% of strains were resistant to trimethoprim, erythromycin, ciprofloxacin, and chloramphenicol, respectively, on all three media. Moreover, 17.9% and 53.8% of all strains were resistant to four and three antibiotics of different antimicrobial classes, respectively. We then looked for antimicrobial resistance genes in the genome sequences of the reference strains. The most common genetic determinant potentially involved in AMR encodes an efflux pump. Since these genes pass through the gastrointestinal tract and may be transferred to human microbiota, further experiments are needed to analyze the probability of this scenario in more detail.

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Metagenomics-Based Analysis of the Age-Related Cumulative Effect of Antibiotic Resistance Genes in Gut Microbiota

Cited by 22 publications

References 88 publications

Washed microbiota transplantation improves patients with high blood glucose in South China

Washed microbiota transplantation improves patients with high blood glucose in South China

Washed microbiota transplantation improves patients with metabolic syndrome in South China

Antimicrobial Resistance of Acetobacter and Komagataeibacter Species Originating from Vinegars

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