Metagenomic Sequence of Prokaryotic Microbiota from an Intermediate-Salinity Pond of a Saltern in Isla Cristina, Spain

Abstract

Marine salterns are artificial multipond systems designed for the commercial production of salt by evaporation of seawater. We report here the metagenomic sequence of the prokaryotic microbiota of a pond with intermediate salinity (21% total salts) of a saltern located in Isla Cristina, Huelva, southwest Spain.

Comparative analysis of prokaryotic diversity in solar salterns in eastern Anatolia (Turkey)

“…Analysis of the average community composition of the four solar salterns showed that the most highly represented genus was Haloquadratum which made up 23.6 % of the total archaeal pyrosequencing reads, followed by Halorubrum (19.6 %), Haloarcula (16.9 %), and Halonotius (16.6 %). OTUs affiliated with Haloquadratum, retrieved in this study, were similar to those obtained from clone libraries recovered from solar salterns in Tunisia, Mexico, and Spain (Baati et al 2008;Dillon et al 2013;Fernández et al 2014). Halonotius-related pyrosequencing reads shared similarities with Halonotius pteroides isolated from Australian crystallizers and clones recovered from salt crystals processed from Mediterranean seawater (Burns et al 2004;Baati et al 2010).…”

“…When comparing our pyrosequencing data with those obtained from Santa Pola, in which microbiota inhabiting ponds of different salinities (13, 19, 37 %) were investigated, Haloquadratum was the most represented archaeal genus in the ponds of Bingöl (25 % salinity) and Santa Pola (37 % salinity). The abundance of OTUs related with Halorubrum and Haloquadratum were notable within the ponds of Tuzlagözü (20 % salinity) and Santa Pola (19 % salinity) both of which also contained Halomicrobium-, Natronomonas-, Halobacterium-, and Haloferax-related OTUs which constituted a lower proportion of the pyrosequencing reads (Ghai et al 2011;Fernández et al 2014). …”

“…Currently, high-throughput sequencing methods allow us to comprehensively analyze the structure of microbial communities from different environmental samples and have recently been used to investigate the microbial compositions of different hypersaline environments (Ghai et al 2011;Fernández et al 2014). In the present work, we used pyrosequencing to reveal the microbial community composition of crystallizers and also investigated the microbiota inhabiting both the crystallizers and feeding waters using the cultivation and FISH-based analyses.…”

From Metagenomics to Pure Culture: Isolation and Characterization of the Moderately Halophilic Bacterium Spiribacter salinus gen. nov., sp. nov

“…The recruitment of the complete genome of strain M19-40 T (29) from metagenomic data sets recently obtained from hypersaline habitats with different salt concentrations (15,30,31) was carried out via BLASTN analysis. A restrictive cutoff of 95% identity in at least 50 bp was established to guarantee that only similarities at the level of nearly identical microbes were counted.…”

Determining virus-host interactions and glycerol metabolism profiles in geographically diverse solar salterns with metagenomics

“…Microbial DNA from the Santa Pola salterns has recently been sampled and sequenced (Fernandez et al, 2013), in addition to microbial DNA from salterns in Isla Cristina, Spain (Fernández et al, 2014b); Cahuil, Chile (Plominsky et al, 2014); and Chula Vista, California (Rodriguez-Brito et al, 2010). Metagenomics has also made it possible to study the vast haloviral populations that inhabit these ecosystems.…”