Metagenomic Reconstruction of Key Anaerobic Digestion Pathways in Municipal Sludge and Industrial Wastewater Biogas-Producing Systems

Cai, Mingwei; Wilkins, David E.; Chen, Jiapeng; Ng, Siu-Kin; Lu, Hsu Feng; Jia, Yangyang; Lee, Patrick K. H.

doi:10.3389/fmicb.2016.00778

Cited by 95 publications

(55 citation statements)

References 89 publications

(143 reference statements)

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“…They also revealed the abundance of genes related to fatty acid and lipid metabolism in anaerobic WWTP having human excreta as a substrate (Cai et al, 2016). On the contrary, our analysis showed the abundance of genes of subsystem “amino-acids and derivatives” and “carbohydrates metabolism”.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Microbial Interactions and Metabolic Potentials in Pre- and Post-treated Sludge from a Wastewater Treatment Plant Using Metagenomic Studies

2017

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Sewage waste represents an ecosystem of complex and interactive microbial consortia which proliferate with different kinetics according to their individual genetic as well as metabolic potential. We performed metagenomic shotgun sequencing on Ion-Torrent platform, to explore the microbial community structure, their biological interactions and associated functional capacity of pre-treated/raw sludge (RS) and post-treated/dried sludge (DS) of wastewater treatment plant. Bacterial phylotypes belonging to Epsilonproteobacteria (∼45.80%) dominated the RS with relatively few Archaea (∼1.94%) whereas DS has the dominance of beta- (30.23%) and delta- (13.38%) classes of Proteobacteria with relatively greater abundance of Archaea (∼7.18%). In particular, Epsilonproteobacteria appears as a primary energy source in RS and sulfur-reducing bacteria with methanogens seems to be in the potential syntrophic association in DS. These interactions could be ultimately responsible for carrying out amino-acid degradation, aromatic compound degradation and degradation of propionate and butyrate in DS. Our data also reveal the presence of key genes in the sludge microbial community responsible for degradation of polycyclic aromatic hydrocarbons. Potential pathogenic microbes and genes for the virulence factors were found to be relatively abundant in RS which clearly reflect the necessity of treatment of RS. After treatment, potential pathogens load was reduced, indicating the sludge hygienisation in DS. Additionally, the interactions found in this study would reveal the biological and environmental cooperation among microbial communities for domestic wastewater treatment.

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Section: Discussionmentioning

confidence: 99%

Unraveling the Microbial Interactions and Metabolic Potentials in Pre- and Post-treated Sludge from a Wastewater Treatment Plant Using Metagenomic Studies

2017

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“…Statistical Analysis of Metagenomic Profiles (STAMP; version 2.1.3 Parks and Beiko, 2010;Cai et al, 2016) was used to compute the abundance differences in the case of whole microbiome and core taxa (http://kiwi.cs.dal.ca/Software/STAMP). Dissimilar taxa were identified with two-sided White's non-parametric t-test (White et al, 2009) at 0.95 confidence intervals and the results with q-value (corrected p-value) of <0.05 were retained.…”

Section: Statistical Analysis Of Metagenomics Datamentioning

confidence: 99%

Characterization of Core Microbiomes and Functional Profiles of Mesophilic Anaerobic Digesters Fed With Chlorella vulgaris Green Microalgae and Maize Silage

et al. 2019

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Microalgal biomass is an alternative feedstock for biogas production although its C/N ratio is usually lower than optimal, therefore co-fermentation is recommended. Biogas production from photoautotrophically grown Chlorella vulgaris (C. vulgaris) biomass (240 mL CH 4 g oTS −1 ) and co-fermentation with maize silage (330 mL CH 4 g oTS −1 ) has been studied in semi continuous laboratory biogas fermenters. Maize silage control yielded 310 mL CH 4 g oTS −1 . The microbial community and the read-based functional profiles, derived from these data, were examined during the process by using next-generation metagenome Ion Torrent sequencing technology. The read-based core microbiome consisted of 92 genera from which 60 abundant taxa were directly associated with the microbial methane producing food chain. The data-set was also analyzed in a genome-based approach. Sixty-five bins were assembled, 52 of them belonged in the core biogas producing genera identified by the read-based metagenomes. The read-based and genome-based approaches complemented and verified each other. The functional profiles indicated a variety of glycoside hydrolases. Substantial rearrangements of the methanogen functions have also been observed. Co-fermentation of algal biomass and plant biomass can be carried out for an extended period of time without process failure. The microbial members of the inoculum are well-conserved, feedstock composition changes caused mostly relative abundance alterations in the core microbiome.

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“…Lysobacter and Pseudomonas , also found in the landfill solid sample inoculum, play major roles as hydrolysers, whereas Clostridium , Bacillus and Paenibacillus contributed by both inocula are cellulolytic microorganisms . Clostridium together with Streptomyces and Bacteroides also participate in acidogenesis . In addition, Clostridium , Bacteroides and Smithella play important roles as acetate producers in syntrophic relationships with methanogens .…”

Section: Resultsmentioning

confidence: 99%

Methane potential and metagenomics of wastewater sludge and a methane‐producing landfill solid sample as microbial inocula for anaerobic digestion of food waste

Sillas‐Moreno

Senés‐Guerrero

Pacheco

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Anaerobic digestion of food waste is influenced by the selection of an adequate microbial inoculum. In this study, wastewater sludge, a methane (CH4)‐producing landfill solid sample and the combination of both [1:1 volatile solid (VS)] were tested as inocula to treat food waste modelled to the Mexican diet. Hence, biochemical methane potential assays at inoculum to substrate (I:S) ratios of 1–3 and each inoculum metagenome were determined. RESULTS Methane production was optimal at I:S = 1 for sludge and the combined inoculum, whereas for landfill solid sample increasing the proportion of inoculum was beneficial up to I:S = 2. The landfill solid sample produced more CH4 than commonly used sludge (311.5 ± 11.1 and 282.5 ± 18.1 mL CH4 g−1 VS, respectively) but presented an adaptation phase (four days). Combining inocula (I:S = 1) produced a synergistic effect in CH4 yield (374.5 ± 10.5 mL CH4 g−1 VS), biogas quality (58.0 ± 0.3% CH4) and COD removal (90.0 ± 0.6%) without start‐up time. Moreover, an enriched microbial community high in Archaea and Bacteria was observed, probably because of the incorporation of predominant members of each inoculum (Bacteroidetes, Firmicutes and Euryarchaeota in sludge, and Proteobacteria and Actinobacteria in landfill solid sample). Archaea was represented mainly by the genus Methanosaeta (order Methanosarcinales), suggesting that methanogenesis occurred by the acetoclastic pathway. CONCLUSION Food waste from Mexican diet was a suitable feedstock for biogas generation. Optimal conditions were observed when combining both inocula. Further studies on population dynamics during digestion would help understand the synergistic effect for effective industrial application. © 2018 Society of Chemical Industry

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Metagenomic Reconstruction of Key Anaerobic Digestion Pathways in Municipal Sludge and Industrial Wastewater Biogas-Producing Systems

Cited by 95 publications

References 89 publications

Unraveling the Microbial Interactions and Metabolic Potentials in Pre- and Post-treated Sludge from a Wastewater Treatment Plant Using Metagenomic Studies

Unraveling the Microbial Interactions and Metabolic Potentials in Pre- and Post-treated Sludge from a Wastewater Treatment Plant Using Metagenomic Studies

Characterization of Core Microbiomes and Functional Profiles of Mesophilic Anaerobic Digesters Fed With Chlorella vulgaris Green Microalgae and Maize Silage

Methane potential and metagenomics of wastewater sludge and a methane‐producing landfill solid sample as microbial inocula for anaerobic digestion of food waste

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