Metagenomic Profiling of Viruses Associated with Rhipicephalus microplus Ticks in Yunnan Province, China

Shi, Junming; Shěn, Shū; Wu, Hui; Zhang, Yunzhi; Deng, Fei

doi:10.1007/s12250-020-00319-x

Cited by 35 publications

(33 citation statements)

References 38 publications

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“…A study from Thailand reported no evidence of an adaptive immune response consistent with infection in parasitized humans and animals to any of the segment deficient viruses identified within the ticks [ 66 ]. In a study from China, the presence of serum antibodies to an M-segment deficient phlebovirus, YN tick-associated phlebovirus 1, was reported in 4% of cattle from an endemic region [ 67 ]. Immunoreactivity was also found in several other viruses recently identified through tick virome analysis that had previously been hypothesized to be tick endosymbionts.…”

Section: Discussionmentioning

confidence: 99%

Virome of Ixodes ricinus, Dermacentor reticulatus, and Haemaphysalis concinna Ticks from Croatia

Sameroff

Tokarz

Vucelja

et al. 2022

Viruses

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Tick-borne diseases are a serious threat to both public and veterinary health. In this study, we used high-throughput sequencing to characterize the virome of three tick species implicated in the spread of vector-borne disease throughout Croatia. Ten viruses were identified, including seven potential novel species within the viral families Flaviviridae, Nyamiviridae, Rhabdoviridae, Peribunyaviridae, Phenuiviridae, and Nairoviridae.

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Section: Discussionmentioning

confidence: 99%

Virome of Ixodes ricinus, Dermacentor reticulatus, and Haemaphysalis concinna Ticks from Croatia

Sameroff

Tokarz

Vucelja

et al. 2022

Viruses

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“…To investigate the diversity and richness of viromes harboured in ticks, we established 10 tick sequencing pools according to the different locations and tick species (Table S2). Total RNA was extracted from the tick pools, as previously described [ 32 ]. RNA from each pool (3 μg) was subjected to RNA sequencing (RNA-seq) using a HiSeq 3000 sequencer according to the manufacturer’s instructions (Illumina, San Diego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Viromes and surveys of RNA viruses in camel-derived ticks revealing transmission patterns of novel tick-borne viral pathogens in Kenya

You

Agwanda

et al. 2021

Emerging Microbes & Infections

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Tick-borne viruses (TBVs) capable of transmitting between ticks and hosts have been increasingly recognized as a global public health concern. In this study, Hyalomma ticks and serum samples from camels were collected using recorded sampling correlations in eastern Kenya. Viromes of pooled ticks were profiled by metagenomic sequencing, revealing a diverse community of viruses related to at least 11 families. Five highly abundant viruses, including three novel viruses (Iftin tick virus, Mbalambala tick virus [MATV], and Bangali torovirus [BanToV]) and new strains of previously identified viruses (Bole tick virus 4 [BLTV4] and Liman tick virus [LMTV]), were characterized in terms of genome sequences, organizations, and phylogeny, and their molecular prevalence was investigated in individual ticks. Moreover, viremia and antibody responses to these viruses have been investigated in camels. MATV, BLTV4, LMTV, and BanToV were identified as viral pathogens that can potentially cause zoonotic diseases. The transmission patterns of these viruses were summarized, suggesting three different types according to the sampling relationships between viral RNA-positive ticks and camels positive for viral RNA and/or antibodies. They also revealed the frequent transmission of BanToV and limited but effective transmission of other viruses between ticks and camels. Furthermore, follow-up surveys on TBVs from tick, animal, and human samples with definite sampling relationships are suggested. The findings revealed substantial threats from the emerging TBVs and may guide the prevention and control of TBV-related zoonotic diseases in Kenya and in other African countries.

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“…Very few reports describe isolation attempts, particularly for insect-associated viruses, while the replication of the prototype strain of the prototype jingmenvirus JMTV could only be detected for a couple of passages on vertebrate (DH82, dog) and insect (C6/36, mosquito) cell lines, or in intracranially injected newborn mice [5]. Isolation has been attempted for other strains of this virus, but all have been unsuccessful or limited to the first couple of passages [8,[12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Discovery of a Novel Jingmenvirus in Australian Sugarcane Soldier Fly (Inopus flavus) Larvae

Colmant

Furlong

Etebari

2022

Viruses

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In Australia, soldier flies are major pests of sugarcane, and they can cause significant yield losses in some areas, possibly due to the virus’ transmission to the plants. We sequenced fly larvae salivary glands and identified a novel jingmenvirus, putatively named Inopus flavus jingmenvirus 1 (IFJV1). Phylogenetic trees confirmed that IFJV1 groups with insect-associated jingmenviruses, newly identified flavivirus-like viruses with a segmented genome. After the design and the validation of molecular detection systems for IFJV1, larval homogenates were passaged on insect and vertebrate cells, but IFJV1 could only be detected in the first two passages in insect cells and not at all in vertebrate cells. Despite this lack of consistent replication in laboratory models, this virus does replicate in its host Inopus flavus, as sequenced, small RNA from the larvae matched the IFJV1 sequences. Moreover, they were found to be predominantly 21 nucleotides long and map to the whole sequences on both strands, which is typical of an actively replicating virus. This discovery confirms the worldwide presence of jingmenviruses which, until now, had only been detected on four continents. However, the study of IFJV1 tropism and the possible pathogenicity to its host or the sugarcane it parasitizes requires the development of a stable replication model.

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Metagenomic Profiling of Viruses Associated with Rhipicephalus microplus Ticks in Yunnan Province, China

Cited by 35 publications

References 38 publications

Virome of Ixodes ricinus, Dermacentor reticulatus, and Haemaphysalis concinna Ticks from Croatia

Virome of Ixodes ricinus, Dermacentor reticulatus, and Haemaphysalis concinna Ticks from Croatia

Viromes and surveys of RNA viruses in camel-derived ticks revealing transmission patterns of novel tick-borne viral pathogens in Kenya

Discovery of a Novel Jingmenvirus in Australian Sugarcane Soldier Fly (Inopus flavus) Larvae

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