Metagenomic of clinically diseased and healthy broiler affected with respiratory disease complex

Patel, J.G.; Patel, B. J.; Patel, Snehal S.; Raval, S.H.; Parmar, R. S.; Joshi, D. V.; Chauhan, H.C.; Chandel, B.S.; Patel, Bharat K.

doi:10.1016/j.dib.2018.05.010

Cited by 13 publications

(13 citation statements)

References 9 publications

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“…Other studies reported similar results [46]. Available at www.veterinaryworld.org/Vol.14/September-2021/8.pdf Our results also showed that the isolates had a high resistance rate (71.4%) for each of (doxycycline and ciprofloxacin), which was in agreement with the results obtained from a study conducted on laying hens in India [47]. Furthermore, 28.5% of the currently isolated strains of ORT were resistant to enrofloxacin.…”

Section: Effect Of Antibiotics On Ortsupporting

confidence: 91%

The first isolation and detection of Ornithobacterium rhinotracheale from swollen head syndrome-infected broiler flocks in Iraq

et al. 2021

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Background and Aim: The swollen head syndrome (SHS) makes up complex diseases that infect the upper respiratory tract in poultry and causes several economic losses. Furthermore, this syndrome is considered one of the multifactorial etiological agents. Therefore, this study isolated and molecularly detected Ornithobacterium rhinotracheale (ORT) in poultry. Materials and Methods: This study was conducted at 67 broiler farms that had birds observed to be infected with the SHS from September 2018 until August 2019. Subsequently, swabs were collected from their trachea, infraorbital sinuses, and lungs, after which obtained samples were treated through two methods: (a) The direct method, by uploading samples on FTA cards, and the indirect method using a transport media. Afterward, reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze the directly treated samples; howeverAQ1, the culture method, followed by PCR, was used to analyze the indirectly treated samples. Next, a partial 16S RNA gene was isolated using four positive PCR products, after which the effect of 16 antibiotics was studied on the seven local ORT strains isolated. Results: The quantity of ORT isolated using the direct method was 28 (41.7%) samples, which were all positive for the strain. Identification was by direct molecular identification (RT-PCR) from samples loaded on FTA cards. Alternatively, 7 (10.4%) ORTs were detected from the indirect method, as obtained using the culture method and biochemical tests. Then, PCR was subsequently used to confirm the results. As observed, 784 bp bands were shown for all seven ORT isolates. Furthermore, results revealed a significant difference in the detection of ORT strains between direct and indirect methods, with p-value (<0.05) and standard deviation of the error ±0.038 for the direct, then ±0.061 for the indirect method. For further analysis on the strain types, four 784 bp PCR products were taken, then partial 16S ribosomal sequence typing was conducted. All these four strains were found to be recorded in NCBI for the 1st time as a local Iraqi strain, with accession numbers (MN931657, MN931656, MN931655, and MN931654). Notably, results also showed that all isolated strains were multidrug-resistant. Conclusion: From the results, ORT is proposed to be implicated as one of the etiological factors that cause SHSs in poultry. Phylogenetic analysis of the current ORT bacterial strains also showed that they are closely related to the Egyptian isolates.

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Section: Effect Of Antibiotics On Ortsupporting

confidence: 91%

The first isolation and detection of Ornithobacterium rhinotracheale from swollen head syndrome-infected broiler flocks in Iraq

et al. 2021

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“…Chicken meat is the second most consumed meat and represents approximately 36% of world meat production. Therefore, poultry respiratory diseases are not only a problem for the biggest producers, but also a global concern [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…The most important etiological agents of poultry respiratory diseases include: (a) viruses (Newcastle disease, avian metapneumovirus, avian infectious bronchitis); (b) fungi, such as Aspergillus spp. ; and (c) bacteria, including Mycoplasma gallisepticum, Pasteurella multocida, Avibacterium paragallinarum, Escherichia coli, Riemerella anatipestifer, Bordetella avium, and Ornithobacterium rhinotracheale [9,10].…”

Section: Introductionmentioning

confidence: 99%

Ornithobacterium rhinotracheale: An Update Review about An Emerging Poultry Pathogen

Barbosa

Cardoso

Silva

et al. 2019

Veterinary Sciences

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Respiratory diseases in birds generate sanitary and economic impacts and may be related to the environment and climate. Mycoplasma gallisepticum, Ornithobacterium rhinotracheale (ORT), Pasteurella multocida, Avibacterium paragallinarum, Escherichia coli, Riemerella anatipestifer, and Bordetella avium are among the most important avian respiratory pathogens. ORT is responsible for causing ornitobacteriosis, a disease characterized by clinical signs ranging from mild to severe respiratory conditions, with high mortality rates, mainly affecting turkeys and chickens. The first report of ornitobacteriosis was in 1981 in Germany. Despite its importance, few studies on ORT have been published. In addition, the presence of this pathogen has been neglected in poultry farms, mainly due to the lack of appropriate diagnostic protocols. The lack of correct isolation and diagnostic protocols along with inappropriate use of antimicrobial agents have been contributing to treatment failure. Due to its economic importance to the poultry industry, ornitobacteriosis should be monitored and included in national programs for the prevention and control of avian respiratory diseases. This review aimed to update and discuss important issues related to ORT since this pathogen has great economic and sanitary implications for the chicken production chain.

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“…Another case in point are the low pathogenic avian influenza viruses (LPAIV) that, during outbreaks, even in the antibiotic era, are coupled to co-infections by pathogens such as Mycoplasma gallisepticum, Mycoplasma synoviae, Ornithobacterium rhinotracheale, avian pathogenic Escherichia coli (APEC) and Staphylococcus aureus, which are responsible for a higher mortality rate (Much et al, 2002;Belkasmi et al, 2020) and a marked reduction in egg production of laying hens (Umar et al, 2016). Moreover, it appears as though that concurrent pathogens such as infectious bronchitis virus (IBV), the APEC, the avian pneumovirus and the LPAIV act synergistically or cumulatively to mediate complex infectious processes (Awad et al, 2014;Guabiraba and Schouler, 2015;Patel et al, 2018).…”

Section: Complex Respiratory Disease and Comorbidities: The Multiple Pathogen Disease Paradigm In A Pathobiome Contextmentioning

confidence: 99%

“…The feline respiratory disease complex has been described as one of the most importance cause of morbidity for cats in U.S.A, with reported incidence as high as 30% (Wagner et al, 2018). Finally, the respiratory disease complex in poultry remains widespread and has become endemic in different countries causing subclinical infections, mild respiratory symptoms and high production losses in birds either raised for meat or eggs (Awad et al, 2014;Guabiraba and Schouler, 2015;Patel et al, 2018;Samy and Naguib, 2018).…”

Section: Introductionmentioning

confidence: 99%

The Airway Pathobiome in Complex Respiratory Diseases: A Perspective in Domestic Animals

Mach

Baranowski

Nouvel

et al. 2021

Front. Cell. Infect. Microbiol.

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Respiratory infections in domestic animals are a major issue for veterinary and livestock industry. Pathogens in the respiratory tract share their habitat with a myriad of commensal microorganisms. Increasing evidence points towards a respiratory pathobiome concept, integrating the dysbiotic bacterial communities, the host and the environment in a new understanding of respiratory disease etiology. During the infection, the airway microbiota likely regulates and is regulated by pathogens through diverse mechanisms, thereby acting either as a gatekeeper that provides resistance to pathogen colonization or enhancing their prevalence and bacterial co-infectivity, which often results in disease exacerbation. Insight into the complex interplay taking place in the respiratory tract between the pathogens, microbiota, the host and its environment during infection in domestic animals is a research field in its infancy in which most studies are focused on infections from enteric pathogens and gut microbiota. However, its understanding may improve pathogen control and reduce the severity of microbial-related diseases, including those with zoonotic potential.

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Metagenomic of clinically diseased and healthy broiler affected with respiratory disease complex

Cited by 13 publications

References 9 publications

The first isolation and detection of Ornithobacterium rhinotracheale from swollen head syndrome-infected broiler flocks in Iraq

The first isolation and detection of Ornithobacterium rhinotracheale from swollen head syndrome-infected broiler flocks in Iraq

Ornithobacterium rhinotracheale: An Update Review about An Emerging Poultry Pathogen

The Airway Pathobiome in Complex Respiratory Diseases: A Perspective in Domestic Animals

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