Metagenomic insights into the effects of volatile fatty acids on microbial community structures and functional genes in organotrophic anammox process

Shu, Duntao; He, Ya‐Ling; Yue, Hong; Zhu, Liang; Wang, Qingyi

doi:10.1016/j.biortech.2015.07.107

Cited by 60 publications

(24 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In all samples, independently of sewer operating condition, the most abundant phyla were Proteobacteria > Bacteroidetes > Firmicutes ( Figure 1A ). Similar results were reported for sludges from China ( Zhang et al, 2012 ; Shu et al, 2015b ; Gao et al, 2016 ; Liang et al, 2017 ). However, the literature shows some contrasting results.…”

Section: Discussionsupporting

confidence: 87%

“… Gao et al (2016) identified a common nucleus of 177 genera for sewage sludges from China. This shared core of bacteria is usually responsible for the main functions in the environment ( Shu et al, 2015b ). Several pathogenic bacteria, such as Clostridium , Treponema , Stenotrophomonas , Bacillus , Mycobacterium , and Acinetobacter were also identified, in accordance to Stiborova et al (2015) .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sewage Sludge Microbial Structures and Relations to Their Sources, Treatments, and Chemical Attributes

et al. 2018

View full text Add to dashboard Cite

Sewage sludges generation and their disposal have become one of the greatest challenges of the 21st century. They have great microbial diversity that may impact wastewater treatment plant (WWTP) efficiency and soil quality whether used as fertilizers. Therefore, this research aimed to characterize microbial community diversity and structure of 19 sewage sludges from São Paulo, Brazil, as well as to draw their relations to sludge sources [domestic and mixed (domestic+industrial)], biological treatments (redox conditions and liming), and chemical attributes, using molecular biology as a tool. All sludges revealed high bacterial diversity, but their sources and redox operating conditions as well as liming did not consistently affect bacterial community structures. Proteobacteria was the dominant phylum followed by Bacteroidetes and Firmicutes; whereas Clostridium was the dominant genus followed by Treponema, Propionibacterium, Syntrophus, and Desulfobulbus. The sludge samples could be clustered into six groups (C1 to C6) according their microbial structure similarities. Very high pH (≥11.9) was the main sludge attribute segregating C6, that presented very distinct microbial structure from the others. Its most dominant genera were Propionibacterium > > Comamonas > Brevundimonas > Methylobacterium ∼Stenotrophomonas ∼Cloacibacterium. The other clusters’ dominant genera were Clostridium > > Treponema > Desulfobulbus ∼Syntrophus. Moreover, high Fe and S were important modulators of microbial structure in certain sludges undertaking anaerobic treatment and having relatively low N-Kj, B, and P contents (C5). However, high N-Kj, B, P, and low Fe and Al contents were typical of domestic, unlimed, and aerobically treated sludges (C1). In general, heavy metals had little impact on microbial community structure of the sludges. However, our sludges shared a common core of 77 bacteria, mostly Clostridium, Treponema, Syntrophus, and Comamonas. They should dictate microbial functioning within WWTPs, except by SS12 and SS13.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Sewage Sludge Microbial Structures and Relations to Their Sources, Treatments, and Chemical Attributes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…They found strong correlations between the N-acetyl-homoserine-lactone (AHL)-12 based quorum sensing system (a highly studied quorum sensing system) and granule formation. While the process is autotrophic, some anammox bacteria are speculated to carry out 16 heterotrophic, or potentially mixotrophic, ammonium oxidation, simultaneously removing 17 carbon and nitrogen (Shu et al, 2015). What is universally recognised is that anammox granules 18 support multiple reactions resulting in the proliferation of a complex, multi-trophic, cooperative 19…”

mentioning

confidence: 99%

Granular Biofilms: Formation, Function, Application, and New Trends as Model Microbial Communities

Trego¹,

Mills²,

Collins³

2019

Preprint

View full text Add to dashboard Cite

As the global demand for water increases, so does the quantity of wastewater requiring treatment. Due to a relatively low carbon footprint, compared with conventional wastewater treatment technologies, anaerobic digestion (AD) was identified in the 1970s as a forerunner in the push for sustainability, when interest in sustainable technologies and renewable energy sources was first sparked. AD technology development ultimately resulted in the discovery of the ‘anaerobic granule’. It is a spontaneously-forming bio-aggregate of microbial cells capable of digesting pollutants and producing methane-rich biogas as a renewable source of bioenergy. The high settling velocity of such granules meant that AD systems could be operated as high-rate treatment processes, because the active, relatively-slow-growing, pollutant-removing biomass would be retained inside, and not washed out of, even bioreactors operated at extremely high volumetric loading rates. In the intervening years the emergence of the anaerobic ammonium oxidising (anammox) granule, aerobic granule, hydrogenic granule, oxygenic photogranule, and many other functionally-specialised granules, has opened new opportunities in wastewater treatment biotechnology. Whilst environmental engineering based around wastewater treatment is still a growing field of research and implementation, the granule (in all forms) is starting to catch the attention of microbial ecologists. It is a self-immobilised biofilm, with many of the properties of ‘conventional’ biofilms formed in Nature. However, as a single entity, a granule represents an entire community of microorganisms, competing or functioning cooperatively or in syntrophy. Together, inside a bioreactor, granules perform side-by-side arguably representing a meta-organism. Granules are gaining traction as the perfect samples for high-throughput studies on fundamental ecological concepts. Granular biofilms can be used to test hypotheses around drivers of diversity, community assembly, biofilm formation and maturation, community expansion and succession, community stress response, among others. This review outlines the history of three of the most influential types of granules: the anaerobic (methanogenic), aerobic and anammox granule. The main biochemical processes found in each type; their primary characteristics; and the typical makeup of the microbial community underpinning the processes are compared. Finally, the adoption of granules as the perfect ‘playground’ for experiments in microbial ecology is reviewed.

show abstract

“…After pyrosequencing, all the raw reads were analyzed using QIIME standard pipeline (Shu et al, 2015) to trim off the low quality reads, adaptors, barcodes and primers. Then, sequences containing ambiguous base calls (Ns) and sequences shorter than 150 bp were also removed.…”

Section: Methodsmentioning

confidence: 99%

Distribution and characteristic of nitrite-dependent anaerobic methane oxidation bacteria by comparative analysis of wastewater treatment plants and agriculture fields in northern China

2016

PeerJ

View full text Add to dashboard Cite

Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered biological process which has been arousing global attention because of its potential in minimizing greenhouse gases emissions. In this study, molecular biological techniques and potential n-damo activity batch experiments were conducted to investigate the presence and diversity of M. oxyfera bacteria in paddy field, corn field, and wastewater treatment plant (WWTP) sites in northern China, as well as lab-scale n-damo enrichment culture. N-damo enrichment culture showed the highest abundance of M. oxyfera bacteria, and positive correlation was observed between potential n-damo rate and abundance of M. oxyfera bacteria. Both paddy field and corn field sites were believed to be better inoculum than WWTP for the enrichment of M. oxyfera bacteria due to their higher abundance and the diversity of M. oxyfera bacteria. Comparative analysis revealed that long biomass retention time, low NH and high NO content were suitable for the growth of M. oxyfera bacteria.

show abstract

Metagenomic insights into the effects of volatile fatty acids on microbial community structures and functional genes in organotrophic anammox process

Cited by 60 publications

References 36 publications

Sewage Sludge Microbial Structures and Relations to Their Sources, Treatments, and Chemical Attributes

Sewage Sludge Microbial Structures and Relations to Their Sources, Treatments, and Chemical Attributes

Granular Biofilms: Formation, Function, Application, and New Trends as Model Microbial Communities

Distribution and characteristic of nitrite-dependent anaerobic methane oxidation bacteria by comparative analysis of wastewater treatment plants and agriculture fields in northern China

Contact Info

Product

Resources

About