Metagenomic and quantitative insights into microbial communities and functional genes of nitrogen and iron cycling in twelve wastewater treatment systems

Shu, Duntao; He, Ya‐Ling; Yue, Hong; Wang, Qingyi

doi:10.1016/j.cej.2016.01.024

Cited by 114 publications

(25 citation statements)

References 30 publications

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“…All PCR reactions were performed in triplicate with 20 μL of the final reaction mixture, which contained 4 μL of 5 × Fast Pfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of Taq DNA Polymerase (Thermo Scientific, USA), 0.2 μL BSA, and 10 ng of template DNA. The thermal cycling conditions were as follows: an initial denaturation step at 95°C for 3 min, and 28 cycles at 95°C for 30 s, 55°C for 30 s, and 72°C for 45 s, followed by a final extension at 72°C for 10 min (Shu et al, 2016 ). PCR products were initially screened using 2% agarose gels and purified using Agencourt AMPure XP (Beckman, USA) according to the instructions.…”

Section: Methodsmentioning

confidence: 99%

Microbial Communities Shaped by Treatment Processes in a Drinking Water Treatment Plant and Their Contribution and Threat to Drinking Water Safety

et al. 2017

Front. Microbiol.

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Bacteria play an important role in water purification in drinking water treatment systems. On one hand, bacteria present in the untreated water may help in its purification through biodegradation of the contaminants. On the other hand, some bacteria may be human pathogens and pose a threat to consumers. The present study investigated bacterial communities using Illumina MiSeq sequencing of 16S rRNA genes and their functions were predicted using PICRUSt in a treatment system, including the biofilms on sand filters and biological activated carbon (BAC) filters, in 4 months. In addition, quantitative analyses of specific bacterial populations were performed by real-time quantitative polymerase chain reaction (qPCR). The bacterial community composition of post-ozonation effluent, BAC effluent and disinfected water varied with sampling time. However, the bacterial community structures at other treatment steps were relatively stable, despite great variations of source water quality, resulting in stable treatment performance. Illumina MiSeq sequencing illustrated that Proteobacteria was dominant bacterial phylum. Chlorine disinfection significantly influenced the microbial community structure, while other treatment processes were synergetic. Bacterial communities in water and biofilms were distinct, and distinctions of bacterial communities also existed between different biofilms. By contrast, the functional composition of biofilms on different filters were similar. Some functional genes related to pollutant degradation were found widely distributed throughout the treatment processes. The distributions of Mycobacterium spp. and Legionella spp. in water and biofilms were revealed by real-time quantitative polymerase chain reaction (qPCR). Most bacteria, including potential pathogens, could be effectively removed by chlorine disinfection. However, some bacteria presented great resistance to chlorine. qPCRs showed that Mycobacterium spp. could not be effectively removed by chlorine. These resistant bacteria and, especially potential pathogens should receive more attention. Redundancy analysis (RDA) showed that turbidity, ammonia nitrogen and total organic carbon (TOC) exerted significant effects on community profiles. Overall, this study provides insight into variations of microbial communities in the treatment processes and aids the optimization of drinking water treatment plant design and operation for public health.

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Section: Methodsmentioning

confidence: 99%

Microbial Communities Shaped by Treatment Processes in a Drinking Water Treatment Plant and Their Contribution and Threat to Drinking Water Safety

et al. 2017

Front. Microbiol.

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“…Thus, understanding genetic characteristics of these recipient bacteria in relation with the aforementioned biological events that affect the success of HGT will be central in improving this plasmid-bioaugmentation approach in wastewater. In this context, new tools such as metagenomics, transcriptomics, proteomics and metabolomics can be helpful in achieving this goal [ 98 , 99 , 100 , 101 ].…”

Section: Potential Strategy To Improve the Efficiency Of Bioaugmenmentioning

confidence: 99%

Bioaugmentation: An Emerging Strategy of Industrial Wastewater Treatment for Reuse and Discharge

Nzila

Razzak

Zhu

2016

IJERPH

161

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A promising long-term and sustainable solution to the growing scarcity of water worldwide is to recycle and reuse wastewater. In wastewater treatment plants, the biodegradation of contaminants or pollutants by harnessing microorganisms present in activated sludge is one of the most important strategies to remove organic contaminants from wastewater. However, this approach has limitations because many pollutants are not efficiently eliminated. To counterbalance the limitations, bioaugmentation has been developed and consists of adding specific and efficient pollutant-biodegrading microorganisms into a microbial community in an effort to enhance the ability of this microbial community to biodegrade contaminants. This approach has been tested for wastewater cleaning with encouraging results, but failure has also been reported, especially during scale-up. In this review, work on the bioaugmentation in the context of removal of important pollutants from industrial wastewater is summarized, with an emphasis on recalcitrant compounds, and strategies that can be used to improve the efficiency of bioaugmentation are also discussed. This review also initiates a discussion regarding new research areas, such as nanotechnology and quorum sensing, that should be investigated to improve the efficiency of wastewater bioaugmentation.

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“…Typical nitrogen removal microorganisms are dominant in Proteobacteria, Bacteroidetes, and Planctomycetes, and they are detected in different sewage treatment processes (Tian et al, ). Proteobacteria have been reported by many studies to contain common denitrifiers and play a major role in N‐cycle for the removal of nitrogen (Shu, He, Yue, & Wang, ; Tan et al, ). Bacteroidetes has been shown to be associated with the degradation of cellulose‐rich organic wastes such as plant residues and agricultural wastes (Dennehy et al, ), and sludge fermentation system (Huang, Dong, Wang, & Feng, ).…”

Section: Resultsmentioning

confidence: 99%

A novel pilot‐scale tubular bioreactor‐enhanced floating treatment wetland for efficient in situ nitrogen removal from urban landscape water: Long‐term performance and microbial mechanisms

Cui

Yang

Ding

et al. 2019

Water Environment Research

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In order to strengthen in situ nitrogen removal of urban landscape water, a novel pilot-scale tubular bioreactor-enhanced floating treatment wetland (TB-EFTW) was constructed, and the long-term performance and responsible microbial mechanisms were investigated in this study. The results showed that the system could remove 81.5% nitrogen from the landscape water after 240 days' operation. Moreover, the contribution rate of plant absorption to nitrogen was low (8.3%), which indicated that microbial biotransformation rather than plant absorption played a more key role in nitrogen removal in TB-EFTW system. The declining dissolved oxygen (DO) concentration along the axial direction of tubular bioreactor (TB) resulted in the sequential bacterial community of nitrifying, aerobic denitrifying, and anoxic denitrifying bacteria in the front, middle, and final part of TB. High-throughput sequencing results demonstrated that the internal environment of the system realized the coexistence of nitrifying, aerobic denitrifying and anoxic denitrifying process. The reason was mainly because that oxic-anoxic (O-A) areas were formed in sequence along the axial direction of tubular bioreactor. Overall, a unique advantage in nitrogen removal was achieved in TB-EFTW, which could provide important references for in situ treatment of urban landscape water. • Practitioner points• TB-EFTW strengthened nitrogen removal for in situ urban landscape water treatment. • Microbial conversion played a key role in nitrogen removal of the TB-EFTW system. • The unique distribution of oxic-anoxic (O-A) areas was formed in sequence along the TB. • Nitrification, aerobic, and anoxic denitrification were synergistically involved in the TB.• Key words agriculture; biological treatment; nitrogen removal; surface water

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Metagenomic and quantitative insights into microbial communities and functional genes of nitrogen and iron cycling in twelve wastewater treatment systems

Cited by 114 publications

References 30 publications

Microbial Communities Shaped by Treatment Processes in a Drinking Water Treatment Plant and Their Contribution and Threat to Drinking Water Safety

Microbial Communities Shaped by Treatment Processes in a Drinking Water Treatment Plant and Their Contribution and Threat to Drinking Water Safety

Bioaugmentation: An Emerging Strategy of Industrial Wastewater Treatment for Reuse and Discharge

A novel pilot‐scale tubular bioreactor‐enhanced floating treatment wetland for efficient in situ nitrogen removal from urban landscape water: Long‐term performance and microbial mechanisms

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