Metagenomic analysis of relative abundance and diversity of bacterial microbiota in Bemisia tabaci infesting cotton crop in Pakistan

Shah, Syed Hamid Jalal; Malik, A. H.; Zhang, Fangfang; Bào, Yīmíng; Qazi, Javaria

doi:10.1016/j.meegid.2020.104381

Cited by 7 publications

(2 citation statements)

References 58 publications

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“…The fecal microbial genomic DNA samples were extracted by QIAamp DNA stool mini kit (QIAGEN) following the manufacturer's instructions. Then, the fecal microbial genomic DNA samples were sent to the Beijing Institute of Genomics, Chinese Academy of Sciences, where metagenomics analysis was performed on an Illumina HiSeq2000 sequencing platform (Illumina Inc.) according to the protocol described in the published research 26 . The species composition and abundance information of the samples were analyzed and counted.…”

Section: Methodsmentioning

confidence: 99%

Piperine improves levodopa availability in the 6‐OHDA‐lesioned rat model of Parkinson's disease by suppressing gut bacterial tyrosine decarboxylase

Hu,

Yu,

et al. 2023

CNS Neurosci Ther

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AimTyrosine decarboxylase (TDC) presented in the gut‐associated strain Enterococcus faecalis can convert levodopa (L‐dopa) into dopamine (DA), and its increased abundance would potentially minimize the availability and efficacy of L‐dopa. However, the known human decarboxylase inhibitors are ineffective in this bacteria‐mediated conversion. This study aims to investigate the inhibition of piperine (PIP) on L‐dopa bacterial metabolism and evaluates the synergistic effect of PIP combined with L‐dopa on Parkinson's disease (PD).MethodsMetagenomics sequencing was adopted to determine the regulation of PIP on rat intestinal microbiota structure, especially on the relative abundance of E. faecalis. Then, the inhibitory effects of PIP on L‐dopa conversion and TDC expression of E. faecalis were tested in vitro. We examined the synergetic effect of the combination of L‐dopa and PIP on 6‐hydroxydopamine (6‐OHDA)‐lesioned rats and tested the regulations of L‐dopa bioavailability and brain DA level by pharmacokinetics study and MALDI‐MS imaging. Finally, we evaluated the microbiota‐dependent improvement effect of PIP on L‐dopa availability using pseudo‐germ‐free and E. faecalis‐transplanted rats.ResultsWe found that PIP combined with L‐dopa could better ameliorate the move disorders of 6‐OHDA‐lesioned rats by remarkably improving L‐dopa availability and brain DA level than L‐dopa alone, which was associated with the effect of PIP on suppressing the bacterial decarboxylation of L‐dopa via effectively downregulating the abnormal high abundances of E. faecalis and TDC in 6‐OHDA‐lesioned rats.ConclusionOral administration of L‐dopa combined with PIP can improve L‐dopa availability and brain DA level in 6‐OHDA‐lesioned rats by suppressing intestinal bacterial TDC.

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Section: Methodsmentioning

confidence: 99%

Piperine improves levodopa availability in the 6‐OHDA‐lesioned rat model of Parkinson's disease by suppressing gut bacterial tyrosine decarboxylase

Hu,

Yu,

et al. 2023

CNS Neurosci Ther

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“…To decode the functional implications of these gut symbionts, it is necessary to study their distribution. Thus, we have summarized recent metagenomics studies (whole insect and insect gut) of several insect pests which is demonstrated by phylum wise composition of bacteria in Figure 1A ( Yong et al, 2017 ; Tokuda et al, 2018 ; Bozorov et al, 2019 ; Gawande et al, 2019 ; Shah et al, 2020 ; Asimakis et al, 2021 ; Deguenon et al, 2021 ; He et al, 2021 ; Tegtmeier et al, 2021 ; Yu et al, 2021 ; Csorba et al, 2022 ; Li S. et al, 2022 ; Majumder et al, 2022 ; Wang et al, 2022 ). We have noticed with taken examples that Proteobacteria, Firmicutes and Actinobacteria were the most dominant phyla present in the insect gut could be due to their diverse roles in nutrition.…”

Section: Symbiotic Microbes: a Boon For Insectsmentioning

confidence: 99%

Role of gut symbionts of insect pests: A novel target for insect-pest control

Rupawate¹,

Roylawar²,

Khandagale

et al. 2023

Front. Microbiol.

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Insects possess beneficial and nuisance values in the context of the agricultural sector and human life around them. An ensemble of gut symbionts assists insects to adapt to diverse and extreme environments and to occupy every available niche on earth. Microbial symbiosis helps host insects by supplementing necessary diet elements, providing protection from predators and parasitoids through camouflage, modulation of signaling pathway to attain homeostasis and to trigger immunity against pathogens, hijacking plant pathways to circumvent plant defence, acquiring the capability to degrade chemical pesticides, and degradation of harmful pesticides. Therefore, a microbial protection strategy can lead to overpopulation of insect pests, which can drastically reduce crop yield. Some studies have demonstrated increased insect mortality via the destruction of insect gut symbionts; through the use of antibiotics. The review summarizes various roles played by the gut microbiota of insect pests and some studies that have been conducted on pest control by targeting the symbionts. Manipulation or exploitation of the gut symbionts alters the growth and population of the host insects and is consequently a potential target for the development of better pest control strategies. Methods such as modulation of gut symbionts via CRISPR/Cas9, RNAi and the combining of IIT and SIT to increase the insect mortality are further discussed. In the ongoing insect pest management scenario, gut symbionts are proving to be the reliable, eco-friendly and novel approach in the integrated pest management.

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