2020
DOI: 10.3389/fmicb.2020.00205
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Metagenomic Analysis of Plant Viruses Associated With Papaya Ringspot Disease in Carica papaya L. in Kenya

Abstract: Carica papaya L. is an important fruit crop grown by small-and large-scale farmers in Kenya for local and export markets. However, its production is constrained by papaya ringspot disease (PRSD). The disease is believed to be caused by papaya ringspot virus (PRSV). Previous attempts to detect PRSV in papaya plants showing PRSD symptoms, using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) procedures with primers specific to PRSV, have not yielded conclusi… Show more

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Cited by 20 publications
(15 citation statements)
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“…Using Illumina MiSeq sequencing, Mumo and colleagues marked the genome sequences of four different viruses, a potyvirus (Moroccan watermelon mosaic virus) and carlavirus , including the Cowpea mild mottle virus (CpMMV) and two other putative carlaviruses related to Cucumber vein-clearing virus (CuVCV) from papaya leaves in Kenyan fields [ 137 ]. This study is the first report demonstrating the presence of these viruses in papaya in Kenya.…”
Section: Methods For Plant Viral Diagnosticmentioning
confidence: 99%
“…Using Illumina MiSeq sequencing, Mumo and colleagues marked the genome sequences of four different viruses, a potyvirus (Moroccan watermelon mosaic virus) and carlavirus , including the Cowpea mild mottle virus (CpMMV) and two other putative carlaviruses related to Cucumber vein-clearing virus (CuVCV) from papaya leaves in Kenyan fields [ 137 ]. This study is the first report demonstrating the presence of these viruses in papaya in Kenya.…”
Section: Methods For Plant Viral Diagnosticmentioning
confidence: 99%
“…HTS allowed elucidating the elusive etiology of some diseases ( Vives et al., 2013 ; He et al., 2015 ), but often it is not possible to find a direct association between the disease and a particular virus ( Tomašechová et al., 2020 ) among those detected in the infected plant. In this case, the diagnostic must be established by fulfilling Koch’s postulates, or at least by finding a tight association between the disease and the presence of a certain virus in field surveys ( Mumo et al., 2020 ).…”
Section: Detection Of Plant Virusesmentioning
confidence: 99%
“…One of the main methodological decisions for pool sequencing is the balance between the number of samples to be pooled and the sequencing depth required to detect all viruses present in the pool. According to the literature, the pool composition of different metagenomic studies for plant viromes varied from four to 50 individual samples per pool [ 9 , 12 , 35 , 36 , 37 , 38 , 39 ]. Skums et al [ 40 ] provided a mathematical approach for a pooling strategy for the massive sequencing of human viruses, since the complex nature of these samples imposed restrictions on the pool design [ 40 ].…”
Section: Discussionmentioning
confidence: 99%