Metagenomic analysis of Aedes aegypti and Culex quinquefasciatus mosquitoes from Grenada, West Indies

Ramos-Niño, Maria E.; Fitzpatrick, Daniel M.; Eckstrom, Korin; Tighe, Scott; Hattaway, Lindsey M.; Hsueh, Andy N.; Stone, Diana M.; Dragon, Julie; Cheetham, Sonia

doi:10.1371/journal.pone.0231047

Cited by 28 publications

(19 citation statements)

References 106 publications

(144 reference statements)

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“…Interestingly, contrasted abundances of Serratia and Pseudomonas genera were also found in adult A. aegypti from the Caribbean island of Grenada [ 88 ] but further studies using a standardized methodology would be required to assess their natural variability in mosquito populations across the Caribbean. Bacteria such as Serratia marcescens have been found to increase the susceptibility of A. aegypti females for dengue virus, while Pseudomonas rhodesiae can inhibit La Crosse virus replication in Aedes albopictus cells [ 89 , 90 ].…”

Section: Discussionmentioning

confidence: 99%

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

et al. 2020

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Aedes aegypti develop in aquatic habitats in which mosquito larvae are exposed to physicochemical elements and microorganisms that may influence their life cycle and their ability to transmit arboviruses. Little is known about the natural bacterial communities associated with A. aegypti or their relation to the biotic and abiotic characteristics of their aquatic habitats. We characterized the physicochemical properties and bacterial microbiota of A. aegypti breeding sites and larvae on Guadeloupe and in French Guiana. In addition, we explored whether geographic location, the type of breeding site and physicochemical parameters influenced the microbiota associated with this mosquito species. We used large-scale 16S rRNA gene sequencing of 160 breeding sites and 147 pools of A. aegypti larvae and recorded 12 physicochemical parameters at the sampled breeding sites. Ordination plots and multiple linear regression were used to assess the influence of environmental factors on the bacterial microbiota of water and larvae. We found territory-specific differences in physicochemical properties (dissolved oxygen, conductivity) and the composition of bacterial communities in A. aegypti breeding sites that influenced the relative abundance of several bacteria genera (e.g., Methylobacterium, Roseoccocus) on the corresponding larvae. A significant fraction of the bacterial Electronic supplementary material The online version of this article (

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Section: Discussionmentioning

confidence: 99%

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

et al. 2020

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“…darling , as well as in Aedes aegypti , Ae. albopictus and the Culex pipiens complex [ 51 , 163 , 173 , 182 – 189 ].…”

Section: Paratransgenesis In Different Groups Of Vectorsmentioning

confidence: 99%

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

et al. 2022

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This article presents an overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors. It first briefly summarises some of the disease-causing pathogens vectored by insects and emphasises the need for innovative control methods to counter the threat of resistance by both the vector insect to pesticides and the pathogens to therapeutic drugs. Subsequently, the state of art of paratransgenesis is described, which is a particularly ingenious method currently under development in many important vector insects that could provide an additional powerful tool for use in integrated pest control programmes. The requirements and recent advances of the paratransgenesis technique are detailed and an overview is given of the microorganisms selected for genetic modification, the effector molecules to be expressed and the environmental spread of the transgenic bacteria into wild insect populations. The results of experimental models of paratransgenesis developed with triatomines, mosquitoes, sandflies and tsetse flies are analysed. Finally, the regulatory and safety rules to be satisfied for the successful environmental release of the genetically engineered organisms produced in paratransgenesis are considered. Graphical Abstract

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“…This includes mosquitoes within Aedes sp., Anopheles sp., and Culex sp. ( Crotti et al, 2009 ; Freece et al, 2014 ; Ramos-Nino et al, 2020 ). Asaia is among the numerically dominant bacterial populations that colonize mosquitoes as assessed by sequencing surveys ( Chouaia et al, 2010 ; Damiani et al, 2010 ) and can be found in larval and adult mosquitoes and multiple tissues (e.g., the adult gut, testes, ovaries, and salivary glands; Favia 2007 , 2008 ; Mancini, 2018 ).…”

Section: A Path Forward: a Most Wanted List For Microbes In Gnotobiotic Studiesmentioning

confidence: 99%

The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions

et al. 2021

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The increasing availability of modern research tools has enabled a revolution in studies of non-model organisms. Yet, one aspect that remains difficult or impossible to control in many model and most non-model organisms is the presence and composition of the host-associated microbiota or the microbiome. In this review, we explore the development of axenic (microbe-free) mosquito models and what these systems reveal about the role of the microbiome in mosquito biology. Additionally, the axenic host is a blank template on which a microbiome of known composition can be introduced, also known as a gnotobiotic organism. Finally, we identify a “most wanted” list of common mosquito microbiome members that show the greatest potential to influence host phenotypes. We propose that these are high-value targets to be employed in future gnotobiotic studies. The use of axenic and gnotobiotic organisms will transition the microbiome into another experimental variable that can be manipulated and controlled. Through these efforts, the mosquito will be a true model for examining host microbiome interactions.

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Metagenomic analysis of Aedes aegypti and Culex quinquefasciatus mosquitoes from Grenada, West Indies

Cited by 28 publications

References 106 publications

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions

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