Metagenome sequencing to unveil microbial community composition and prevalence of antibiotic and metal resistance genes in hypersaline and hyperalkaline Lonar Lake, India

Chakraborty, Jaya; Sapkale, Vibhavari; Shah, Manan; Rajput, Vinay; Mehetre, Gajanan T.; Agawane, Sachin B.; Kamble, Sanjay P.; Dharne, Mahesh

doi:10.1016/j.ecolind.2019.105827

Cited by 37 publications

(11 citation statements)

References 78 publications

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“…The relative abundance of Proteobacteria in the three regions was relatively high, which indicated that Proteobacteria were not sensitive to the extreme habitat of the plateau. This was consistent with the result of many previous studies which found Proteobacteria to be the dominant phylum in lakes (Jiang et al, 2019;Chakraborty et al, 2020). The…”

Section: Bacterial Community Composition and Driverssupporting

confidence: 93%

“…However, the geographical factors showed insignificant impact on bacterial community structure in YRH (Supplementary Table S2), which might be due to the small geographical range of the sampled lakes in this region. It was worth noting that the contribution of uncertain factors reached 43.36%, implying that some unmeasured environmental factors might be vital as well in affecting the bacterial community composition of the lakes (Williams 1998;Chakraborty et al, 2020;Gu et al, 2020;Liu et al, 2021). Distance decay patterns of bacterial community are the results of environmental filtering (environmental factors) and dispersal limitation (geographic factors).…”

Section: Frontiers In Environmental Sciencementioning

confidence: 99%

“…At the class level, the dominant bacteria in the QD and YRH regions were Gamma-proteobacteria, and the dominant bacteria in HX was Bacteroidia (Supplementary Figure S3B). The class of Gamma-Proteobacteria, one member of Proteobacteria phyla, was known as the typical anti-disturbance taxa and the most abundant class present in eutrophic lakes, which contributed to nitrogen fixation, sulfur metabolism, methane metabolism, and salinity reduction of brackish lakes (Zhang et al, 2020b;Chakraborty et al, 2020;. However, previous research reported that the dominant species was Beta-proteobacteria (37.2%) in a Tibet glacial stream and lake (Gu et al, 2020).…”

Section: Figurementioning

confidence: 99%

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Differences in bacterial diversity, composition, and community networks in lake water across three distinct regions on the Qinghai-Tibet Plateau

Wang

Huang

Zhang

et al. 2022

Front. Environ. Sci.

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We analyze bacterial composition, diversity, geographical distribution, and their community networks in lake water in three adjacent regions on the Qinghai-Tibet Plateau (QTP). Results show that bacterial alpha-diversity indices are much lower in the Hoh Xil (HX) than that in the Yellow River Headwater (YRH) regions and the Qaidam (QD) region. The dominant phyla in QD and YRH are Proteobacteria which account for 42.45 % and 43.64 % of all detected phyla, while Bacteroidetes is the dominant bacterial taxa in HX (46.07 %). Redundancy analysis results suggest that the most important factors in driving bacterial community composition in the three regions are altitude (QD), total nitrogen (YRH), and pH (HX), respectively. Both environmental factors and spatial factors significantly affect the bacterial community composition in QD and HX, while only environmental factors are the major drivers in YRH. Finally, network analyses reveal that the bacterial network structure in QD is more complex than those in YRH and HX, whereas the bacterial network in HX is the most stable, followed by those in QD and YRH.

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Section: Bacterial Community Composition and Driverssupporting

confidence: 93%

Section: Frontiers In Environmental Sciencementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Differences in bacterial diversity, composition, and community networks in lake water across three distinct regions on the Qinghai-Tibet Plateau

Wang

Huang

Zhang

et al. 2022

Front. Environ. Sci.

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“…The metagenome-based analysis of microorganisms in Wanfeng Lake sediments showed that Planctomycetes, Proteobacteria, Euryarchaeota, Chloro exi, Bacteroidetes, Acidobacteria, Verrucomicrobia, Actinobacteria, Nitrospirae, Cyanobacteria, Candidate_division_NC10 and Firmicutes were the main microorganisms in all samples. Similar phenomena have been found in other lakes (Huang et al, 2019;Chen et al, 2019;Chakraborty et al, 2020). The total abundance of Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes in the sediments of Daihai Lake, Inner Mongolia, was more than 95% of all samples, with Proteobacteria exceeding 75% (Du et al, 2022).…”

Section: The Community Structure Abundance Of Microbial In the Sedime...supporting

confidence: 86%

Characteristics of antibiotic resistance genes and microbial community distribution in Wanfeng Lake, upper Pearl River, China

Zhang

et al. 2023

Environ Sci Pollut Res

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Wanfeng Lake, a highland lake in the upper part of the Pearl River Basin, has long been disturbed by aquaculture and human activities, resulting in the accumulation of antibiotics and antibiotic resistance genes (ARGs), which pose a major threat to humans and animals.In this study, 20 antibiotics, 9 ARGs, 2 mobile genetic elements (intl1 and intl2) and microbial community structure were investigated in Wanfeng Lake. The results of the study showed that the total concentration of antibiotics in surface water was 372.72 ng/L, with o oxacin (OFX) having the highest concentration (169.48 ng/L), posing a high ecological risk to aquatic organisms. The total concentration of antibiotics in sediments was235.86 ng/g, with umequine (FLU) having the highest concentration (122.54 ng/g).Thisindicates that the main type of antibiotics in Wanfeng Lake are quinolones. Real-time uorescence quantitative PCR analysis results of the relative abundance of ARGs in both surface water and sediments showed that sulfonamide resistance genes > macrolide resistance genes > tetracycline resistance genes > quinolone resistance genes, indicating that sulfonamide resistance genes were the dominant type. The metagenomic results showed that the predominant microorganisms in the sediment under the phylum level were Planctomycetes, Proteobacteria, Euryarchaeota and Chloro exi. Pearson's correlation analysis showed a signi cantly positive correlation between antibiotics and environmental factors with ARGs in Wanfeng Lake and a signi cant positive correlation between antibiotics and ARGs with microorganisms in sediments. This suggests that there is a potential pressure of antibiotics on ARGs, while microorganisms provide the driving force for the evolution and spread of ARGs. This study provides a basis for further research on the occurrence and spread of antibiotics and ARGs in Wanfeng Lake.

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“…Thus, the present study aimed to enrich and investigate nitrate-reducing microorganisms at the cathode of BES from a haloalkaline environment of the Lonar Lake (located in Buldhana District, Maharashtra, India). The Lonar crater lake is a hypersaline soda lake rich in various nutrients and supports a wide diversity of haloalkaliphilic microorganisms ( Paul et al., 2016 ; Antony et al., 2013 ; Chakraborty et al., 2020 ). We first enriched the haloalkaliphilic nitrate-reducing microbial biofilm at the cathode of BES by using an electrochemical cultivation approach.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical enrichment of haloalkaliphilic nitrate-reducing microbial biofilm at the cathode of bioelectrochemical systems

et al. 2021

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Summary Electrotrophic microorganisms have not been well studied in extreme environments. Here, we report on the nitrate-reducing cathodic microbial biofilm from a haloalkaline environment. The biofilm enriched via electrochemical approach under 9.5 pH and 20 g NaCl/L salinity conditions achieved current density and nitrate reduction efficiency via partial and complete denitrification. Voltammetric characterization of the biocathodes revealed a redox center with 0.294 0.003 ( vs. Ag/AgCl) formal potential putatively involved in the electron uptake process. The lack of soluble redox mediators and hydrogen-driven nitrate reduction suggests direct-contact cathodic electron uptake by the nitrate-reducing microorganisms in the enriched biofilm. 16S-rRNA amplicon sequencing of the cathodic biofilm revealed the presence of unreported Pseudomonas, Natronococcus, and Pseudoalteromonas spp. at , and relative sequence abundances, respectively. The enriched nitrate-reducing microorganisms also reduced nitrate efficiently using soluble electron donors found in the lake sediments, thereby suggesting their role in N-cycling in such environments.

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Metagenome sequencing to unveil microbial community composition and prevalence of antibiotic and metal resistance genes in hypersaline and hyperalkaline Lonar Lake, India

Cited by 37 publications

References 78 publications

Differences in bacterial diversity, composition, and community networks in lake water across three distinct regions on the Qinghai-Tibet Plateau

Differences in bacterial diversity, composition, and community networks in lake water across three distinct regions on the Qinghai-Tibet Plateau

Characteristics of antibiotic resistance genes and microbial community distribution in Wanfeng Lake, upper Pearl River, China

Electrochemical enrichment of haloalkaliphilic nitrate-reducing microbial biofilm at the cathode of bioelectrochemical systems

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