Metagenome datasets from women with polycystic ovary syndrome from Irkutsk, Eastern Siberia, Russia

Belkova, Natalia; Klimenko, E. S.; Babaeva, N. I.; Самбялова, А. Ю.; Markova, Daria; Igumnov, Ilia; Suturina, Larisa

doi:10.1016/j.dib.2020.106137

Cited by 2 publications

(2 citation statements)

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“…In this study, using a metagenomic-based methodology, we characterized the diversity, composition, and functional repertoire of the gut viromes of 50 PCOS patients and 43 healthy women. Multivariate analyses revealed that the overall structure of the gut virome was changed in PCOS patients, with considerably high effect size, consistent with previous observations for the PCOS bacteriome (Guo et al, 2016; Liu et al, 2017;Qi et al, 2019;Belkova et al, 2020). Our study strengthened the previous bacteriome study on PCOS (Qi et al, 2019) by adding new information about the virome.…”

Section: Discussionsupporting

confidence: 90%

Metagenomic-based characterization of the gut virome in patients with polycystic ovary syndrome

Huang

Guo

et al. 2022

Front. Microbiol.

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BackgroundPolycystic ovary syndrome (PCOS) is a complex disease that afflicts women of reproductive age, and its pathological mechanism has not been well explained. The gut microbiota is believed to be closely related to the development of PCOS. Although an important component of the gut microbiome, the role of the gut virome in the development of PCOS is still unclear.MethodsIn this study, we profiled and compared the gut viral community of 50 patients with PCOS and 43 healthy women based on the analysis of their fecal whole-metagenome dataset.ResultsThe gut virome of PCOS patients exhibited a significant decrease in within-sample viral diversity and a remarkable alteration of the overall virome composition compared with that of healthy controls. At the family level, Siphoviridae was significantly depleted in the gut virome of patients, while Quimbyviridae was enriched. We identified 1,089 viral operational taxonomic units (vOTUs) that differed in relative abundance between the two groups, of which 455 vOTUs were enriched in PCOS patients (including numerous Bacteroidaceae phages) and 634 were enriched in controls (including numerous viruses predicted to infect Oscillospiraceae, Prevotellaceae, and Ruminococcaceae). Functional comparison of the PCOS-enriched and control-enriched vOTUs uncovered the viral functional signatures associated with PCOS. Furthermore, we demonstrated gut viral signatures for disease discrimination and achieved an area under the receiver operator characteristic curve (AUC) of 0.938, demonstrating the potential of the gut virome in the prediction of PCOS.ConclusionOur findings reveal specific alterations in viral diversity and taxonomic and functional compositions of the gut virome of PCOS patients. Further studies on the etiology of PCOS and the gut viral community will offer new prospects for treating and preventing PCOS and its related diseases.

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Section: Discussionsupporting

confidence: 90%

Metagenomic-based characterization of the gut virome in patients with polycystic ovary syndrome

Huang

Guo

et al. 2022

Front. Microbiol.

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“…Polycystic ovary syndrome (PCOS) is a female endocrine disease of unclear origin, characterized by hyperandrogenism, oligoovulation or anovulation, and ovarian cysts [11][12][13]. The relationship between disease and microbiome dysbiosis has been shown in model studies performed on rats [14].…”

Section: Introductionmentioning

confidence: 99%

Does Salivary Microbiome Reflect The Functional Activity Profile Of Oral Microbiota?

et al. 2022

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Human oral cavity is a diverse habitat, consisting of many locations with its microbiotas. It was proven that bacteria detected in saliva could be the indicators of disease and be useful for diagnosis, monitoring, and overall assessment of the patient health. As pilot research of microbial communities associated with polycystic ovary syndrome (PCOS), biomaterials were collected from two patients (saliva, the contents of gingival pockets) for sequencing the amplicon libraries V4-V6 of variable regions of the 16S rRNA gene. Our study aimed at comparing the taxonomic and functional profiles of different locations in the oral cavity. Material and Methods — DNA samples were sequenced using Illumina MiSeq; the qiime2-2020.2 and PICRUSt2 v2.4.1 software packages were used to process the sequencing results of the amplicon libraries. Results — We demonstrated that the salivary microbiome had a greater taxonomic diversity, compared with the microbiome of the periodontal pockets. Regarding the ratios of different taxonomic group abundances, the species ratio in the saliva community significantly differed from the ratio in periodontal pockets. The microbiota of the oral cavity was classified as a producing community, since many different biosynthetic pathways were predicted. Similar functional features were identified for microbial communities in other locations. Conclusions — Different locations in the biotope of the oral cavity have varying species richness of their communities and specific taxonomic composition. However, the microbiotas of different microniches perform similar metabolic functions. This finding allows considering the analysis of saliva microbiota sufficiently representative tool for characterization of the entire oral microbiome.

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Metagenome datasets from women with polycystic ovary syndrome from Irkutsk, Eastern Siberia, Russia

Cited by 2 publications

References 15 publications

Metagenomic-based characterization of the gut virome in patients with polycystic ovary syndrome

Metagenomic-based characterization of the gut virome in patients with polycystic ovary syndrome

Does Salivary Microbiome Reflect The Functional Activity Profile Of Oral Microbiota?

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