Metacyclogenesis of Leishmania (Viannia) braziliensis in vitro: evidence that lentil lectin is a marker of complement resistance and enhanced infectivity

Almeida, Marcos Célio de; Cuba, César Augusto Cuba; de, Cesar M.; Pharoah, Mark M.; Howard, Keith; Miles, Michael A.

doi:10.1016/0035-9203(93)90150-o

Cited by 21 publications

(23 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Membrane composition has been shown to in¯uence the ability of Leishmania to infect macrophages (Ebrahimzadeh and Jones 1983;Almeida et al 1993). Membrane composition has been shown to in¯uence the ability of Leishmania to infect macrophages (Ebrahimzadeh and Jones 1983;Almeida et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Cell surface carbohydrates and in vivo infectivity of glucantime-sensitive and resistant Leishmania (Viannia) guyanensis cell lines

et al. 2001

View full text Add to dashboard Cite

The cell surface plays an important role in the interaction of parasites with their hosts. Drug resistance in the protozoan Leishmania may involve changes in cell-surface composition, although it is not known whether infectivity is also aected. One sensitive and two glucantime-resistant lines of Leishmania (Viannia) guyanensis previously isolated were inoculated into hamsters. The sensitive line caused the disease to manifest earlier than the resistant lines. Imprinting analyses of infected macrophages showed that the sensitive line was more infective than the resistant cell lines. In vitro drug resistance was evaluated and the comparative analyses of dose±response curves showed that the susceptibility pattern of the sensitive line did not change after passage in animals, but a decrease in drug resistance was observed in resistant cell lines recovered from the mammalian host. Cell surface carbohydrates of sensitive and resistant cell lines were analysed before and after passage in animals by agglutination tests with several plant lectins. Passage in animals changed the agglutination pattern for many lectins from all three cell lines. Loss of reactivity to lectins seemed to be correlated with a decrease in infectivity of the parasite-resistant cell lines. This study opens possibilities for exploring the relationship between drug susceptibility, infectivity and surface carbohydrate composition of protozoan parasites.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell surface carbohydrates and in vivo infectivity of glucantime-sensitive and resistant Leishmania (Viannia) guyanensis cell lines

et al. 2001

View full text Add to dashboard Cite

show abstract

“…However, in contrast to LPG of other Leishmania species, metacyclics of L. braziliensis make less LPG and add β1,3-glucose residues that branch off the disaccharide-phosphate repeat units of LPG. Metacyclics of L. braziliensis are selectively agglutinated by the mannose specific lentil lectin [53], and are not recognized by Bauhinia purpurea lectin, which has affinity by β-Gal(1,3)-GalNAc residues [54]. …”

Section: Metacyclogenesismentioning

confidence: 99%

Sand Fly-Leishmania Interactions: Long Relationships are Not Necessarily Easy

Ramalho-Ortigão

Saraiva²,

Traub-Csekö³

2010

TOPARAJ

View full text Add to dashboard Cite

Sand fly and Leishmania are one of the best studied vector-parasite models. Much is known about the development of these parasites within the sand fly, and how transmission to a suitable vertebrate host takes place. Various molecules secreted by the vector assist the establishment of the infection in a vertebrate, and changes to the vector are promoted by the parasites in order to facilitate or enhance transmission. Despite a generally accepted view that sand flies and Leishmania are also one of the oldest vector-pathogen pairs known, such long history has not been translated into a harmonic relationship. Leishmania are faced with many barriers to the establishment of a successful infection within the sand fly vector, and specific associations have been developed which are thought to represent aspects of a co-evolution between the parasite and its vectors. In this review, we highlight the journey taken by Leishmania during its development within the vector, and describe the issues associated with the natural barriers encountered by the parasite. Recent data revealed sexual replication of Leishmania within the sand fly, but it is yet unknown if such reproduction affects disease outcome. New approaches targeting sand fly molecules to prevent parasite transmission are being sought, and various techniques related to genetic manipulation of sand flies are being utilized.

show abstract

“…Lo anterior sugiere que aunque no se observen cambios morfológicos evidentes en los promastigotes de fase estacionaria, algunos marcadores de metaciclogénesis, como el grado de aglutinación frente a algunas lectinas, reflejan que efectivamente se está dando una transformación hacia una forma infectiva. La generación de promastigotes infectivos dependiente de la fase de crecimiento se ha demostrado también en especies de Leishmania donovani, Leishmania major, Leishmania tropica, Leishmania amazonensis y Leishmania braziliensis (7,8,11,12,14).…”

Section: Evidencias De Un Estadio Infectivo En Promastigotes De Leishunclassified

“…Se han desarrollado protocolos similares para purificar promastigotes metacíclicos por medio de anticuerpos monoclonales específicos de LPG procíclico en L. tropica (13) y L. donovani (14). Recientemente, Spath y Beverly (15) diseñaron un método de separación de formas metacíclicas, independiente de LPG, el cual mostró buena eficacia y correlación con el método basado en aglutinación con PNA; sin embargo, su utilidad práctica en otras especies diferentes a L. major aún no se ha evaluado.…”

Section: Evidencias De Un Estadio Infectivo En Promastigotes De Leishunclassified

Metaciclogénesis: un proceso fundamental en la biología de Leishmania

Muskus¹,

Marín-Villa²

2002

biomedica

View full text Add to dashboard Cite

La metaciclogénesis es un proceso que experimentan naturalmente los promastigotes de Leishmania en el tracto digestivo del insecto vector y cuya finalidad es transformar los promastigotes en formas altamente infectivas y capaces de sobrevivir en el hospedero vertebrado, donde es sometido a los ataques por parte del sistema inmune. Se ha demostrado que este fenómeno ocurre también en promastigotes en crecimiento en cultivos axénicos in vitro. El proceso de metaciclogénesis se asoció inicialmente con cambios morfológicos, observándose que los promastigotes cambiaban su forma y tamaño con incremento en la longitud del flagelo. Luego, se logró asociar con este fenómeno la expresión de ciertas moléculas implicadas en virulencia, como el lipofosfoglicano (LPG) y una proteasa de superficie, la gp63. Se demostró que estas moléculas experimentaban cambios tanto cuantitativos como cualitativos a medida que los promastigotes se diferencian de promastigotes procíclicos no infectivos a metacíclicos o infectivos. Hoy en día, mediante técnicas de hibridización substractiva con cADN o de amplificación diferencial, se han logrado identificar genes cuyo patrón de expresión está íntimamente ligado al proceso de metaciclogénesis. Se han identificado moléculas como el producto del gen B y una proteína asociada con la metaciclogénesis (Mat-1), las cuales se expresan exclusivamente en promastigotes metacíclicos, mientras que otras como las proteínas Meta-1, SHERP y HASP se sobrexpresan en los promastigotes metacíclicos. Sin embargo, la función y asociación de estas proteínas con este patrón particular de expresión y virulencia se está empezando a evaluar.Palabras clave: metaciclogénesis, Leishmania, promastigotes, LPG, gp63. Metacyclogenesis: a basic process in Leishmania biologyMetacyclogenesis is a process whereby Leishmania transforms from poorly infective procyclic promastigotes into highly infective metacyclic promastigotes. In nature, metacyclogenesis occurs in the insect vector. This transformation is accompanied by an increased ability to infect and survive in the vertebrate host, where the parasite is attacked by the host's immune system. Metacyclogenesis has also been shown to occur in axenic cultures of promastigotes. Morphological changes in size and shape, and length of flagellum were first associated with differentiation in the insect gut and in different phases of growth in culture. Later, the expression of molecules such as LPG and the surface protease gp63 were associated with this process. These two molecules were observed to undergo qualitative and quantitative modifications as the promastigotes differentiated from procyclic to metacyclic forms. Using cDNA subtractive hybridization-based methods or differential amplification, previously unknown genes tightly linked to metacyclogenesis have been identified. Gene products exclusively expresed in metacyclic promastigotes included a gene B product and Mat-1 -a gene associated with metacyclogenesis. Other proteins, Meta-1, SHERP and HASP, were up-regulated during the met...

show abstract

Metacyclogenesis of Leishmania (Viannia) braziliensis in vitro: evidence that lentil lectin is a marker of complement resistance and enhanced infectivity

Cited by 21 publications

References 18 publications

Cell surface carbohydrates and in vivo infectivity of glucantime-sensitive and resistant Leishmania (Viannia) guyanensis cell lines

Cell surface carbohydrates and in vivo infectivity of glucantime-sensitive and resistant Leishmania (Viannia) guyanensis cell lines

Sand Fly-Leishmania Interactions: Long Relationships are Not Necessarily Easy

Metaciclogénesis: un proceso fundamental en la biología de Leishmania

Contact Info

Product

Resources

About