Metabolomics to study the sublethal effects of diazepam and irbesartan on glass eels (Anguilla anguilla)

Alvarez-Mora, Iker; Bolliet, Valérie; Lopez-Herguedas, Naroa; Olivares, Maitane; Monperrus, Mathilde; Etxebarria, Néstor

doi:10.1016/j.aquatox.2023.106547

Cited by 2 publications

(3 citation statements)

References 47 publications

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“…Dysregulation in the levels of twelve lipids, most of which have functions related to energy and structural functions, was observed. It has been emphasized that this situation may be related to oxidative stress, inflammation, or changes in energy metabolism (Alvarez‐Mora et al., 2023 ). Although studies have shown that chronic doses of diazepam slow down weight gain (Sonn et al., 2022 ), the underlying cause is not fully known.…”

Section: Discussionmentioning

confidence: 99%

Behavior, antioxidant, and metabolomics effects of Allium tuncelianum

Abbak,

Nemutlu,

Reçber

et al. 2024

Food Science & Nutrition

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Allium species are consumed extensively as folkloric medicine and dietary elements, but limited studies have been conducted on them. In this study, the effects of an ethanol–water extract obtained from the underground bulb of Allium tuncelianum (Kollmann) Özhatay, B. Mathew & Şiraneci (AT) on the behavioral, antioxidant, and metabolite parameters in rats were evaluated. AT was administered orally once a day at doses of 100 and 400 mg/kg to male Wistar albino rats for 10 consecutive days. The elevated plus maze, rotarod, and hotplate tests were used to examine anxiety‐like behaviors, locomotor activities, and pain perception in the rats, respectively. Additionally, untargeted metabolomic analyses were performed on plasma samples and AT extracts using two orthogonal analytical platforms. The phenolic components, mainly fumaric acid, malic acid, vanillic acid, quercetin‐3‐arabinoside, hydrocinnamic acid, and gallocatechin, were determined in the extract. In addition, arbutin, salicylic acid, trehalose, and nicotinic acid were analyzed in the extract for the first time. The AT extract did not decrease the catalase, glutathione peroxidase, or superoxide dismutase levels; however, diazepam decreased some of those parameters significantly in the brain, liver, and kidney. Although both the AT and diazepam treatments resulted in an increase in anxiolytic‐like effects compared to the control group, no significant differences were observed (p > .05). In the metabolomic analysis, significant changes were observed in the rats treated with AT and diazepam, and they caused significant changes in some metabolic pathways, including amino acid and fatty acid metabolism, compared to the control.

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Section: Discussionmentioning

confidence: 99%

Behavior, antioxidant, and metabolomics effects of Allium tuncelianum

Abbak,

Nemutlu,

Reçber

et al. 2024

Food Science & Nutrition

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“…Briefly, 500 µL of plasma were transferred to a 2 mL Precellys® tube and were spiked with 20 µL of internal standard solution, containing deuterated endocannabinoids and lipidic mediators in a concentration of 1.25 µg/mL in acetonitrile, as described in the previous work 23 . For the extraction, 1000 µL of methyl tert-butyl ether (MTBE, HPLC grade, Lab-Scan Analytical Science, Poland) were added to the tubes, and the mixture was homogenized in a Precellys® 24 homogenizer coupled to a Cryolys (Bertin Technologies, France) at 4°C with 3 cycles of 20 seconds at 6500 rpm and 20 seconds of rest between cycles.…”

Section: Analysis Of Plasma Samplesmentioning

confidence: 99%

“…Since the studied cohorts were large enough, that work opened the possibility to broaden the scope and to address a more in-depth study based on non-targeted analysis to find key biomarkers beyond the ones determined through target analysis approach. For this reason, we have implemented a metabolomic approach to study the lipidic phase isolated in the previous work by means of high performance liquid chromatography coupled to high resolution mass spectrometry (HPLC-HRMS), as it has been carried out previously 23,24 . This way, a better understanding of the relation between cannabis abuse and SZ development would be achieved and potential biomarkers could be suggested.…”

Section: Introductionmentioning

confidence: 99%

Differential Serum Metabolomic Profile in Patients with Schizophrenia, Cannabis Use Disorder or Dual diagnosis

Uriguen,

Villate,

Olivares

et al. 2023

Preprint

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Cannabis use disorder occurs in up to 42% of subjects with schizophrenia. It has been associated with a lower age of onset of schizophrenia, more positive symptoms and a higher frequency of hospitalizations. A number of metabolomic-based studies have shown that levels of several amino acids and lipids are altered in plasma of schizophrenia patients. Similarly, different levels of various lipids, amino acids, sugars, proteins and other metabolites have been quantified in plasma of cannabis consumers, suggesting that consumption of cannabis does interfere in the regulation of further metabolic pathways. In the present study, we performed a non-targeted metabolomic approach in plasma samples of subjects with a diagnosis of schizophrenia, cannabis use disorder or dual diagnosis. The aim was to find potential biomarkers of the diseases and to gain a better understanding of the relation between cannabis abuse and the development of schizophrenia. The liquid chromatography-high resolution mass spectrometry outcomes allowed the annotation of 121 metabolites. The analysis between each group and the corresponding control samples showed a clearer clustering of the samples. The two-way ANOVA and linear analysis of the whole dataset showed that most of the analytes were differently present between the groups, but none of them seems to correlate to gender or age. It can be highlighted the presence of two endocannabinoids (palmitylethanolamide (PEA) and oleoylethanolamide (OEA)), nicotine and cotinine as biomarkers of tobacco consumption, and nor-9- carboxy-Δ9-THC as biomarker of the consumption of cannabis. Moreover, some medium chain acylcarnitines (C8/C10) share a common pattern in the three comparison groups whereas some N-acyl serines were also highlighted in some of the group comparisons. The use of this non-targeted metabolomic approach provided us a deep insight to evaluate the metabolomics profile and to uncover putative biomarkers in schizophrenia and cannabis use disorder.

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Metabolomics to study the sublethal effects of diazepam and irbesartan on glass eels (Anguilla anguilla)

Cited by 2 publications

References 47 publications

Behavior, antioxidant, and metabolomics effects of Allium tuncelianum

Behavior, antioxidant, and metabolomics effects of Allium tuncelianum

Differential Serum Metabolomic Profile in Patients with Schizophrenia, Cannabis Use Disorder or Dual diagnosis

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