Metabolomics Study Reveals Biomarker L-Proline as Potential Stress-Protectant Compound for High-Temperature Bioethanol Fermentation by Yeast Pichia kudriavzevii 1P4

Khotimah, Husnul; Astuti, Rika Indri; Rafi, Mohamad; Yuliana, Nancy Dewi

doi:10.1007/s12010-023-04554-2

Cited by 1 publication

(3 citation statements)

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“…Pichia spp., is one of the non-conventional yeast group that shows potential characteristics for bioethanol fermentation. Indeed, previous study reported that P. kudriavzevii 1P4 was tolerance towards high temperature (42°C) and could utilize pentose sugar (Ulya et al 2021;Khotimah et al 2023). Utilization of pentose sugar is important in the second generation of bioethanol productions using lignocellulose as substrate.…”

Section: Journal Of Biosciences Journal Of Biosciencesmentioning

confidence: 99%

“…It is worth noting that yeast S. cerevisiae is not capable of using pentose as sugar source. Previous studies have reported the production of ethanol production by yeast P. stipitis (Bellido et al 2011;Li et al 2011;Koti et al 2016), P. kudriavzevii (Astuti et al 2018;Chamnipa et al 2018;Pongcharoen et al 2018;Khotimah et al 2023), and Wickerhamomyces anomalus (P. anomala) (Atitallah et al 2020) using various type of substrates including xylose, hemicellulose, and lignocellulose hydrolysates.…”

Section: Journal Of Biosciences Journal Of Biosciencesmentioning

confidence: 99%

“…Quantitation of bioethanol production was done by using previous method (Khotimah et al 2023). Yeast cells were cultured in YPDB media with 2% selected sugar as carbon source (glucose, xylose, and maltose).…”

Section: Bioethanol Productionmentioning

confidence: 99%

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Bioethanol Production from Non-Conventional Yeasts Wickerhamomyces anomalus (Pichia anomala) and Detection of ADH1 Gene

Fathiah,

Hartono,

Astuti

et al. 2023

HAYATI J Biosci

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Bioethanol is an organic compound resulted from the fermentation of sugar substrates by microorganisms which is used as alternative energy sources. During bioethanol fermentation yeast are exposed to various fermentation stresses, including temperature, osmotic, and oxidative stresess. Such conditions may decrease ethanol production. We previously isolated fermentation-stress tolerance yeast isolates from traditional Balinese beverages, identified as Wickerhamomyces anomalus BT2, BT5, and BT6. However no data available regarding the bioethanol production of those isolates. Our study indicates that these strains could utilize various sugar substrates (glucose, xylose, maltose, sucrose) in oxidative fermentative media. The highest value of substrate utilization efficiency following 48 hours fermentation was shown by BT6 on glucose (61.02%), BT 2 on xylose (55.44%) and maltose (60.90%). Measurement of ethanol production by Gas Chromatography showed that the strains were able to produce higher ethanol on the glucose substrate than other substrates. For instance, BT6 could produce the highest ethanol production (5.00 g/L) amongst strains tested by using glucose as substrate. Yet, the particular strains could only produce 0.30 g/L and 0.65 g/L by using xylose and maltose, respectively. For further genetic engineering purposes, we detected ADH1 gene from all three isolates, with high homology to the alcohol dehydrogenase from Saccharomyces cerevisiae, Geobacillus stearothermophilus and Pseudomonas aeruginosa. Further strain development can be carried out targeting the ADH1 gene, important for ethanol fermentation.

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Section: Journal Of Biosciences Journal Of Biosciencesmentioning

confidence: 99%