Metabolomics Study of Immune Responses of New Zealand Greenshell™ Mussels (Perna canaliculus) Infected with Pathogenic Vibrio sp.

Nguyen, Thao V.; Alfaro, Andrea C.; Young, Tim; Ravi, Sridevi; Mérien, Fabrice

doi:10.1007/s10126-018-9804-x

Cited by 84 publications

(33 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To analyze the tissue distribution of TcMyD88, the following tissues were collected from six healthy adult T. crocea: siphonal mantle, pedal mantle, inner mantle, byssus gland, pedis, heart, gill and hemocytes. For analysis of the developmental expression patterns of TcMyD88, samples of 0, 0.5, 1,4,12,16,24,48,72,96, and 120 h-developing embryos were also obtained.…”

Section: Animals Tissue Embryonic Development Collection and Challementioning

confidence: 99%

“…It has been reported that gross pathological changes to the velum and cilia occurred in diseased Crassostrea gigas larvae [15]. Moreover, V. coralliilyticus has also been associated with outbreaks of vibriosis in several other bivalve species, including Eastern oysters (Crassostrea virginica), the European flat oyster (Ostrea edulis), the great scallop (Pecten maximus), the Atlantic bay scallop (Argopecten irradians) and New Zealand green-lipped mussels (Perna canaliculus) [16,17]. Consequently, V. coralliilyticus is a significant pathogen for bivalves, contributing to dramatic losses in mollusks worldwide.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanistic molecular responses of the giant clam Tridacna crocea to Vibrio coralliilyticus challenge

Zhao

Zhou

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

Vibrio coralliilyticus is a pathogen of coral and mollusk, contributing to dramatic losses worldwide. In our study, we found that V. coralliilyticus challenge could directly affect adult Tridacna crocea survival; there were dead individuals appearing at 6 h post infection, and there were 45.56% and 56.78% mortality rates in challenged groups after 36 h of infection. The apoptosis rate of hemocytes was significantly increased by 1.8-fold at 6 h after V. coralliilyticus injection. To shed light on the mechanistic molecular responses of T. crocea to V. coralliilyticus infection, we used transcriptome sequencing analysis and other relevant techniques to analyze T. crocea hemocytes at 0 h, 6 h, 12 h and 24 h after V. coralliilyticus challenge. Our results revealed that the total numbers of unigenes and DEGs were 195651 and 3446, respectively. Additional details were found by KEGG pathway enrichment analysis, where DEGs were significantly enriched in immune-related signaling pathways, such as the TLR signaling pathway, and some were associated with signaling related to apoptosis. Quantitative validation results illustrated that with exposure to V. coralliilyticus, the expression of TLR pathway members, TLR, MyD88, IRAK4, TRAF6, and IкB-α, were significantly upregulated (by 22.9-, 9.6-, 4.0-, 3.6-, and 3.9-fold, respectively) at 6 h. The cytokine-related gene IL-17 exhibited an increase of 6.3-fold and 10.5-fold at 3 h and 6 h, respectively. The apoptosis-related gene IAP1 was dramatically increased by 2.99-fold at 6 h. These results indicate that adult T. crocea could initiate the TLR pathway to resist V. coralliilyticus, which promotes the release of inflammatory factors such as IL-17 and leads to the activation of a series of outcomes, such as apoptosis. The response mechanism is related to the T. crocea immunoreaction stimulated by V. coralliilyticus, providing a theoretical basis for understanding T. crocea immune response mechanisms.

show abstract

Section: Animals Tissue Embryonic Development Collection and Challementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanistic molecular responses of the giant clam Tridacna crocea to Vibrio coralliilyticus challenge

Zhao

Zhou

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Gas chromatography coupled with mass spectrometry (GC/MS) is a well-established analytical method that can provide a comprehensive and systematic understanding of all metabolites in biological samples (Tsugawa et al, 2011;Nguyen & Alfaro, 2019a). GC/MS-based metabolomics has been widely applied to study the physiological responses of aquatic organisms to environmental stressors, including pathogen infection, water contaminants and aerial exposure, and many metabolites and associated pathways have been successfully identified (Guo et al, 2014;Ji et al, 2016;Chen et al, 2015;Nguyen et al, 2018aNguyen et al, , 2018c, Alfaro, Nguyen & Mellow, 2019.…”

Section: Introductionmentioning

confidence: 99%

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Jiang

Jiao

Sun

et al. 2020

PeerJ

View full text Add to dashboard Cite

Marine mollusks are commonly subjected to heat stress. To evaluate the effects of heat stress on the physiological metabolism of the ark shell Scapharca subcrenata, clams were exposed to different high temperatures (24, 28 and 32 °C) for 72 h. The oxygen consumption and ammonia excretion rates were measured at 2, 12, 24, 48 and 72 h. The results indicated that the metabolic rates of the ark shell significantly increased with increasing heat stress, accompanied by mortalities in response to prolonged exposure. A metabolomics approach based on gas chromatography coupled with mass spectrometry was further applied to assess the changes of metabolites in the mantle of the ark shell at 32 °C. Moreover, multivariate and pathway analyses were conducted for the different metabolites. The results showed that the heat stress caused changes in energy metabolism, amino acid metabolism, osmotic regulation, carbohydrate metabolism and lipid metabolism through different metabolic pathways. These results are consistent with the significant changes of oxygen consumption rate and ammonia excretion rate. The present study contributes to the understanding of the impacts of heat stress on intertidal bivalves and elucidates the relationship between individual-level responses and underlying molecular metabolic dynamics.

show abstract

“…coralliilyticus/neptunius-like isolate induced perturbations of the immune system, oxidative stress, inflammation and metabolic changes at 6 days p.i. [34]. Overall, these findings provide a further insight into the pathogenic effects of V. coralliilyticus in mussels.…”

Section: Coralliilyticus Possess Several Virulence Mechanisms Incmentioning

confidence: 58%

Responses of Mytilus galloprovincialis to challenge with the emerging marine pathogen Vibrio coralliilyticus

Balbi

Auguste

Cortese

et al. 2019

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Vibrio coralliilyticus (V.c.) has emerged as a coral pathogen of concern throughout the Indo-Pacific reef. The interest towards understanding its ecology and pathogenic potential has increased since V.c. was shown to be strongly virulent also for other species; in particular, it represents a serious threat for bivalve aquaculture, being one of the most important emerging pathogen responsible for oyster larval mortalities worldwide. V.c. has a tightly regulated temperature-dependent virulence and it has been related to mass mortalities events of benthic invertebrates also in the temperate northwestern Mediterranean Sea. However, no data are available on the effects of V.c. in the mussel Mytilus galloprovincialis, the most abundant aquacultured species in this area. In this work, responses of M. galloprovincialis to challenge with V.c. (ATCC BAA-450) were investigated. In vitro, short term responses of mussel hemocytes were evaluated in terms of lysosomal membrane stability, bactericidal activity, lysozyme release, ROS and NO production, and ultrastructural changes, evaluated by TEM. In vivo, hemolymph parameters were measured in mussels challenged with V.c. at 24h p.i. Moreover, the effects of V.c. on mussel early embryo development (at 48 hpf) were evaluated. The results show that both in vitro and in vivo, mussels were unable to activate immune response towards V.c., and that challenge mainly induced lysosomal stress in the hemocytes. Moreover, V.c. showed a strong and concentration-dependent embryotoxicity. Overall, the results indicate that, although M. galloprovincialis is considered a resistant species to vibrio infections, the emerging pathogen V.c. can represent a potential threat to mussel aquaculture.

show abstract

Metabolomics Study of Immune Responses of New Zealand Greenshell™ Mussels (Perna canaliculus) Infected with Pathogenic Vibrio sp.

Cited by 84 publications

References 91 publications

Mechanistic molecular responses of the giant clam Tridacna crocea to Vibrio coralliilyticus challenge

Mechanistic molecular responses of the giant clam Tridacna crocea to Vibrio coralliilyticus challenge

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Responses of Mytilus galloprovincialis to challenge with the emerging marine pathogen Vibrio coralliilyticus

Contact Info

Product

Resources

About