Metabolomics Study of Cultivated Bulbus Fritillariae Cirrhosae at Different Growth Stages using UHPLC‐QTOF‐MS Coupled with Multivariate Data Analysis

Geng, Zhao; Liu, Yifei; Yan, Gou; Zhou, Qin‐Mei; He, Chengjun; Guo, Li; Zhou, Juan; Xiong, Liang

doi:10.1002/pca.2742

Cited by 28 publications

(17 citation statements)

References 28 publications

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“…They successfully screened, identified and quantified five markers for the precise quality evaluation of crude and carbonized Pollen Typhae . Geng et al [16] developed a powerful strategy based on high-resolution quadrupole time-of-flight (Q-TOF) MS to explore the chemical ingredients and transformation mechanisms in cultivated Bulbus Fritillariae cirrhosae . They identified four alkaloid compounds that could be used as potential chemical markers for the classification of cultivated Bulbus Fritillariae cirrhosae samples at different growth stages.…”

Section: Introductionmentioning

confidence: 99%

A Study on Tissue-Specific Metabolite Variations in Polygonum cuspidatum by High-Resolution Mass Spectrometry-Based Metabolic Profiling

Wang

Chen

et al. 2019

Molecules

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Polygonum cuspidatum Sieb. et Zucc. is a traditional Chinese herbal medicine widely used to treat tussis, hepatitis and arthralgia. This study identified and quantitatively described the bioactive compounds in different P. cuspidatum tissues. Metabolic profiles of root, stem, leaf, flower, rhizome and seed were determined using high-resolution mass spectrometry in combination with multivariate analyses. In total, 53 metabolites, 8 reported for the first time in this species, were putatively identified and classified mainly as stilbenes, anthraquinones and flavonoids. A principal component analysis, cluster analysis and heatmap were used to depict the correlations between specimens and the relative abundance levels of these compounds in different plant tissues. An orthogonal partial least square discriminant analysis found that 13 metabolites showed distinct differences among the six plant tissues, making them potential discriminative tissue-identification markers. This study will provide guidance in comparing, selecting and exploiting the medicinal uses of different P. cuspidatum tissues.

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Section: Introductionmentioning

confidence: 99%

A Study on Tissue-Specific Metabolite Variations in Polygonum cuspidatum by High-Resolution Mass Spectrometry-Based Metabolic Profiling

Wang

Chen

et al. 2019

Molecules

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“…Although LC–MS can be used to analyze trace components, good sample pretreatment, including extraction and enrichment, is the key to discover trace active components. The steroidal alkaloids in Fritillaria species were identified and characterized using LC/ESI-TOF-MS [ 157 – 159 ], HPLC–MS method [ 158 , 160 ], LC-ESI-QQQ-MS [ 159 , 161 ], RRLC-Q-TOF-MS coupled with multivariate statistical analysis [ 162 ], and UPLC-QTOF-MS [ 163 , 164 ] with metabolomic strategy, which were used to determine potential markers for quality control at different growth stages [ 165 ]. In addition, the major alkaloids in F. hupehensis were determined by HPLC-ELSD and HPLC-ESI-MS n [ 166 ].…”

Section: Analytical Techniques For Chemical Evaluation Of Fritillaria Speciesmentioning

confidence: 99%

Natural drug sources for respiratory diseases from Fritillaria: chemical and biological analyses

et al. 2021

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Fritillaria naturally grows in the temperate region of Northern Hemisphere and mainly distributes in Central Asia, Mediterranean region, and North America. The dried bulbs from a dozen species of this genus have been usually used as herbal medicine, named Beimu in China. Beimu had rich sources of phytochemicals and have extensively applied to respiratory diseases including coronavirus disease (COVID-19). Fritillaria species have alkaloids that act as the main active components that contribute multiple biological activities, including anti-tussive, expectorant, and anti-asthmatic effects, especially against certain respiratory diseases. Other compounds (terpenoids, steroidal saponins, and phenylpropanoids) have also been identified in species of Fritillaria. In this review, readers will discover a brief summary of traditional uses and a comprehensive description of the chemical profiles, biological properties, and analytical techniques used for quality control. In general, the detailed summary reveals 293 specialized metabolites that have been isolated and analyzed in Fritillaria species. This review may provide a scientific basis for the chemical ecology and metabolomics in which compound identification of certain species remains a limiting step.

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“…Despite the wide application of UHPLC with high‐resolution MS in the comprehensive profiling of the constituents of herbal medicines, it remains a challenge to identify all the potential active constituents in less studied folk herbs. Traditional methods include studying the MS and MS 2 data from an existing database or studying the fragmentation behavior of several reported compounds and using those data to determine the structure of an unknown component 16,17 . There are two obvious shortcomings of this conventional method.…”

Section: Introductionmentioning

confidence: 99%

Systematic characterization of alkaloids in Eomecon chionantha Hance using ultrahigh‐performance liquid chromatography–tandem quadrupole Exactive Orbitrap mass spectrometry with a four‐step screening strategy

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Eomecon chionantha Hance (ECH), a traditional folk herb, is commonly used to treat traumatic injuries based on its analgesic and anti‐inflammatory properties. Previous studies have reported that alkaloids are the major bioactive components in ECH. Therefore, identification of alkaloids from ECH contributes to the discovery of its potential active ingredients and quality control in clinic treatments. Methods A four‐step screening strategy was performed as follows. (1) Extracting the accurate masses of ions related to different molecules. (2) Screening different types of compounds using their molecular cations, protonated molecules, diagnostic product ions and fragmentation pathways. (3) Comparing the characteristic product ion formulae to obtain the type and number of substituents. (4) Using the biosynthetic pathways of isoquinoline alkaloids to determine the concentration of alkaloids. Results Ultrahigh‐performance liquid chromatography–tandem quadrupole Exactive Orbitrap mass spectrometry (UHPLC/Q‐Exactive Orbitrap MS) analysis combined with the four‐step screening strategy was used to profile the alkaloids in ECH. The structures of 95 alkaloids in ECH were unambiguously identified or reasonably assigned, of which 76 were reported in ECH for the first time. Six types of benzylisoquinoline alkaloids were identified in ECH: six benzyltetrahydroisoquinolines, nine protopines, five N‐methyltetrahydroprotoberberines, six protoberberines, eight benzophenanthridines and sixty‐one dihydrobenzophenanthridines. Conclusions This comprehensive study identified the alkaloids in ECH, thus providing a practical reference for further research. The UHPLC/Q‐Exactive Orbitrap MS method, combined with the four‐step screening strategy, which was developed and successfully applied to identify the alkaloids in ECH, may also be applicable for the efficient screening of other herbal medicines.

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Metabolomics Study of Cultivated Bulbus Fritillariae Cirrhosae at Different Growth Stages using UHPLC‐QTOF‐MS Coupled with Multivariate Data Analysis

Abstract: The proposed analytical method in combination with multivariate statistical analysis comprised a useful and powerful strategy to explore the chemical ingredients and transforming mechanisms of cultivated BFC and for quality evaluation and control. Copyright © 2018 John Wiley & Sons, Ltd.

Cited by 28 publications

References 28 publications

A Study on Tissue-Specific Metabolite Variations in Polygonum cuspidatum by High-Resolution Mass Spectrometry-Based Metabolic Profiling

A Study on Tissue-Specific Metabolite Variations in Polygonum cuspidatum by High-Resolution Mass Spectrometry-Based Metabolic Profiling

Natural drug sources for respiratory diseases from Fritillaria: chemical and biological analyses

Systematic characterization of alkaloids in Eomecon chionantha Hance using ultrahigh‐performance liquid chromatography–tandem quadrupole Exactive Orbitrap mass spectrometry with a four‐step screening strategy

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