Metabolomics reveals the “Invisible” detoxification mechanisms of Amaranthus hypochondriacus at three ages upon exposure to different levels of cadmium

Xie, Min; Dai, Haibo; Liu, Jiaxin; Xue, Zhe; Chen, Yi; Liu, Xuan; Mou, Haiyan; Sun, Hui; Ao, Tianqi; Li, Yunzhen; Chen, Wenqing

doi:10.1016/j.ecoenv.2020.110520

Cited by 29 publications

(14 citation statements)

References 43 publications

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“…Navarro-Reig et al [283] identified 112 metabolites in rice through LC-MS (HPLC joined with Q-Exactive mass spectrometer). Mengdi et al [284] identified nine metabolic pathways in A. hypochondriacus (K472) through LC-MS analysis. The intermediate vegetative stage had the highest Cd tolerance ability, which expressed 29 significantly different metabolites involved in 4 metabolic processes, including purine metabolism; Gly, Thr, and Ser metabolism; Asp, Ala, and Glu metabolism; and Arg and Pro metabolism.…”

Section: Proteomicsmentioning

confidence: 99%

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Raza

Habib

Najafi-Kakavand

et al. 2020

Biology

170

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Cadmium (Cd) is one of the most toxic metals in the environment, and has noxious effects on plant growth and production. Cd-accumulating plants showed reduced growth and productivity. Therefore, remediation of this non-essential and toxic pollutant is a prerequisite. Plant-based phytoremediation methodology is considered as one a secure, environmentally friendly, and cost-effective approach for toxic metal remediation. Phytoremediating plants transport and accumulate Cd inside their roots, shoots, leaves, and vacuoles. Phytoremediation of Cd-contaminated sites through hyperaccumulator plants proves a ground-breaking and profitable choice to combat the contaminants. Moreover, the efficiency of Cd phytoremediation and Cd bioavailability can be improved by using plant growth-promoting bacteria (PGPB). Emerging modern molecular technologies have augmented our insight into the metabolic processes involved in Cd tolerance in regular cultivated crops and hyperaccumulator plants. Plants’ development via genetic engineering tools, like enhanced metal uptake, metal transport, Cd accumulation, and the overall Cd tolerance, unlocks new directions for phytoremediation. In this review, we outline the physiological, biochemical, and molecular mechanisms involved in Cd phytoremediation. Further, a focus on the potential of omics and genetic engineering strategies has been documented for the efficient remediation of a Cd-contaminated environment.

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Section: Proteomicsmentioning

confidence: 99%

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Raza

Habib

Najafi-Kakavand

et al. 2020

Biology

170

View full text Add to dashboard Cite

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“…It has been proved that the availability of metal ions can be affected by metal-binding ligands . Therefore, the influence of the food matrix on the bioavailability and the toxicity of Cd 2+ is an important issue that should be addressed . Generally, the toxicity of Cd 2+ is affected by substances that bind with them.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Cytotoxicity of Cadmium by a Sulfated Polysaccharide from Abalone

Wang

Song

et al. 2020

J. Agric. Food Chem.

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Consumption of seafood is a common route of cadmium ion (Cd2+) exposure to consumers. The seafood matrices may alter the toxicity profile of Cd2+ due to the interaction between Cd2+ and biomacromolecules in seafood. In this study, enhanced cytotoxicity of Cd2+ was found in the presence of an abalone gonad sulfated polysaccharide (AGSP) and the mechanism was investigated at a metabolic level. The formation of the AGSP–Cd2+ complex was demonstrated by isothermal titration calorimetry. The level of reactive oxygen species (ROS) increased and mitochondrial membrane potential reduced upon exposure to the AGSP–Cd2+ complex as compared with those of Cd2+ exposure. The decreased cell viability after incubation with the AGSP–Cd2+ complex also suggested enhanced Cd2+ toxicity induced by AGSP. The metabolomics and lipidomics analysis revealed that, compared with the Cd2+ group, the AGSP–Cd2+ downregulated the phospholipid metabolism and resulted in more serious damage in the cellular membrane. The lipid metabolism disorder, in turn, amplified the generation of ROS, leading to a decrease in cell viability. These results provided new evidence of the enhanced Cd2+ toxicity upon interaction with seafood polysaccharides, and much attention should be paid to the effect of food ingredients on heavy metal ion toxicity.

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“…For example, cysteine‐rich proteins can significantly enhance Cd resistance [12]. Amino acid metabolisms have been linked to Cd tolerance in plants [13]. The mechanisms for this may be related to physiological responses, including antioxidant production, osmotic balance adjustments, increased energy production, and so on [13].…”

Section: Introductionmentioning

confidence: 99%

“…Amino acid metabolisms have been linked to Cd tolerance in plants [13]. The mechanisms for this may be related to physiological responses, including antioxidant production, osmotic balance adjustments, increased energy production, and so on [13]. However, the amino acids contributing to the defense of oxidative stress by Cd in cells are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Protective role of l‐threonine against cadmium toxicity in Saccharomyces cerevisiae

et al. 2021

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Environment and food contamination with cadmium (Cd) can cause serious toxicity, posing a severe threat to agricultural production and human health. However, how amino acids contribute to defenses against oxidative stress caused by Cd in cells is not fully understood. As a model eukaryote with a relatively clear genetic background, Saccharomyces cerevisiae has been commonly used in Cd toxicity research. To gain insight into Cd toxicity and cell defenses against it, 20 amino acids were screened for protective roles against Cd stress in S. cerevisiae. The results showed that threonine (Thr, T) had the strongest protective effect against Cd‐induced mortality and membrane damage in the cells. Compared to the antioxidant vitamin C (VC), Thr exhibited a higher efficacy in restoring the superoxide dismutase (SOD) activity that was inhibited by Cd but not by H2O2 in vivo. Thr exhibited evident DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) activity but weak ABTS (2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐9 sulfonic acid)) scavenging activity, giving it a weaker effect against Cd‐induced lipid peroxidation and superoxide radical O2−, compared to VC. More importantly, compared to the chelating agent EDTA, Thr showed stronger chelation of Cd, giving it a stronger protective effect on SOD against Cd than VC in vitro. The results of the in vivo and in vitro experiments revealed that the role Thr plays in cell defenses against Cd may be attributed to its protection of the SOD enzyme, predominantly through the preferential chelation of Cd. Our results provide insights into the protective mechanisms of amino acid Thr that ameliorate Cd toxicity and suggest that a supplement of Thr might help to reduce Cd‐induced oxidative damage.

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Metabolomics reveals the “Invisible” detoxification mechanisms of Amaranthus hypochondriacus at three ages upon exposure to different levels of cadmium

Cited by 29 publications

References 43 publications

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Enhanced Cytotoxicity of Cadmium by a Sulfated Polysaccharide from Abalone

Protective role of l‐threonine against cadmium toxicity in Saccharomyces cerevisiae

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