Metabolomics Reveals Dynamic Metabolic Changes Associated with Age in Early Childhood

Chiu, Chih‐Yung; Yeh, Kuo‐Wei; Lin, Gigin; Chiang, Meng-Han; Yang, Shu-Chen; Chao, Wei-Ju; Yao, Tsung‐Chieh; Tsai, Ming‐Han; Hua, Mau‐Sun; Liao, Sui‐Ling; Lai, Shen‐Hao; Cheng, Mei‐Ling; Huang, Jing‐Long

doi:10.1371/journal.pone.0149823

Cited by 63 publications

(92 citation statements)

References 40 publications

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“…Biological age (as opposed to calendar age) is greatly influenced by several factors, such as diet, stress, environment, lifestyle, genetics and disease [47]. Since these factors play important roles in determining the metabolome, urinary metabolomics may provide reliable and sensitive markers to understand the complexity of age-related changes, leading to the identification of novel treatments or strategies for the management of health and disease in early childhood [48]. These age-specific data may also be used for the establishment of inflection points related to metabolic disorders, which is very important in accurate disease diagnosis or prognosis.…”

Section: The Importance Of Age-specific Intervalsmentioning

confidence: 99%

“…This is often not fully realized, due to the fact that the majority of clinical studies that have been published are for adults [49][50][51][52][53][54][55]. Indeed, to date, there have only been a few comprehensive studies looking at age-specific intervals in children [48,56,57] and newborns [30].…”

Section: The Importance Of Age-specific Intervalsmentioning

confidence: 99%

“…Indeed, these metabolites differ significantly between younger and older groups [54]. Urinary trimethylamine-N-oxide (TMAO) has been found to be higher in infants (aged 1 week-1 year), which may be directly associated to the consumption of milk at this age and/or the corresponding gut microflora found in infants, due to breast feeding [48].…”

Section: The Importance Of Age-specific Intervalsmentioning

confidence: 99%

“…Urinary glycine and glutamine levels have been reported to decline significantly within the first year of life, probably due to their use in supporting the increased growth of skeletal muscle tissue during infancy [48]. Another important metabolite measured in urine that changes with age is creatinine (Ucreat).…”

Section: The Importance Of Age-specific Intervalsmentioning

confidence: 99%

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The Urinary Metabolome of Healthy Newborns

et al. 2020

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The knowledge of normal metabolite values for neonates is key to establishing robust cut-off values to diagnose diseases, to predict the occurrence of new diseases, to monitor a neonate's metabolism, or to assess their general health status. For full term-newborns, many reference biochemical values are available for blood, serum, plasma and cerebrospinal fluid. However, there is a surprising lack of information about normal urine concentration values for a large number of important metabolites in neonates. In the present work, we used targeted tandem mass spectrometry (MS/MS)-based metabolomic assays to identify and quantify 136 metabolites of biomedical interest in the urine from 48 healthy, full-term term neonates, collected in the first 24 h of life. In addition to this experimental study, we performed a literature review (covering the past eight years and over 500 papers) to update the references values in the Human Metabolome Database/Urine Metabolome Database (HMDB/UMDB). Notably, 86 of the experimentally measured urinary metabolites are being reported in neonates/infants for the first time and another 20 metabolites are being reported in human urine for the first time ever. Sex differences were found for 15 metabolites. The literature review allowed us to identify another 78 urinary metabolites with concentration data. As a result, reference concentration values and ranges for 378 neonatal urinary metabolites are now publicly accessible via the HMDB.

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Section: The Importance Of Age-specific Intervalsmentioning

confidence: 99%

Section: The Importance Of Age-specific Intervalsmentioning

confidence: 99%

Section: The Importance Of Age-specific Intervalsmentioning

confidence: 99%

Section: The Importance Of Age-specific Intervalsmentioning

confidence: 99%

See 2 more Smart Citations

The Urinary Metabolome of Healthy Newborns

et al. 2020

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“…In a metabolomics study, the detection of global metabolic profiles is preferred to obtain a comprehensive understanding of the biological pathways. The NMR method detects low levels of molecular metabolites in complex biological samples due to its high reproducibility and robust statistics [22,26]. Therefore, a 1 H NMR-based metabolomics approach was employed to reveal the metabolite alterations after the combination treatment with budesonide and salbutamol in asthmatic children.…”

Section: Characteristics Of Patient Groupsmentioning

confidence: 99%

Distinct Metabolic Profile of Inhaled Budesonide and Salbutamol in Asthmatic Children during Acute Exacerbation

Yang

Zhang

et al. 2017

Basic Clin Pharma Tox

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Inhaled budesonide and salbutamol represent the most important and frequently used drugs in asthmatic children during acute exacerbation. However, there is still no consensus about their resulting metabolic derangements; thus, this study was conducted to determine the distinct metabolic profiles of these two drugs. A total of 69 children with asthma during acute exacerbation were included, and their serum and urine were investigated using high-resolution nuclear magnetic resonance (NMR). A metabolomics analysis was performed using a principal component analysis and orthogonal signal correction-partial least squares using SIMCA-P. The different metabolites were identified, and the distinct metabolic profiles were analysed using MetPA. A high-resolution NMR-based serum and urine metabolomics approach was established to study the overall metabolic changes after inhaled budesonide and salbutamol in asthmatic children during acute exacerbation. The perturbed metabolites included 22 different metabolites in the serum and 21 metabolites in the urine. Based on an integrated analysis, the changed metabolites included the following: increased 4-hydroxybutyrate, lactate, cis-aconitate, 5-hydroxyindoleacetate, taurine, trans-4-hydroxy-l-proline, tiglylglycine, 3-hydroxybutyrate, 3-methylhistidine, glucose, cis-aconitate, 2-deoxyinosine and 2-aminoadipate; and decreased alanine, glycerol, arginine, glycylproline, 2-hydroxy-3-methylvalerate, creatine, citrulline, glutamate, asparagine, 2-hydroxyvalerate, citrate, homoserine, histamine, sn-glycero-3-phosphocholine, sarcosine, ornithine, creatinine, glycine, isoleucine and trimethylamine N-oxide. The MetPA analysis revealed seven involved metabolic pathways: arginine and proline metabolism; taurine and hypotaurine metabolism; glycine, serine and threonine metabolism; glyoxylate and dicarboxylate metabolism; methane metabolism; citrate cycle; and pyruvate metabolism. The perturbed metabolic profiles suggest potential metabolic reprogramming associated with a combination treatment of inhaled budesonide and salbutamol in asthmatic children.Glucocorticoids and beta2 agonists represent the most important and frequently used drugs for symptom control in asthmatic children during acute exacerbation [1,2]. Current guidelines recommend the combined use of glucocorticoids and beta2 agonists as the preferred therapy for asthmatic children during acute exacerbation [3,4]. The glucocorticoid and beta2 agonist therapies are effective at controlling airway inflammation, reducing asthma symptoms, decreasing airway hyper-responsiveness and reducing the frequency and severity of exacerbations as well as asthma mortality. Despite their undoubted effectiveness and safety, there is still no consensus about the resulting metabolic derangements, including the development of dyslipidaemia, insulin resistance, glucose intolerance, diabetes, central adiposity and skeletal muscle wasting [5][6][7].Both glucocorticoids and beta2 agonists have the potential to influence glucose, lipid and protein...

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