Metabolomics profiling of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata extracts using UPLC-Q/TOF-MS

Abstract

BackgroundThe side effects caused by Polygoni Multiflori Radix (PMR) and Polygoni Multiflori Radix Praeparata (PMRP) have often appeared globally. There is no research on the changes of endogenous metabolites among PMR- and PMRP-treated rats. The aim of this study was to evaluate the varying metabolomic effects between PMR- and PMRP-treated rats. We tried to discover relevant differences in biomarkers and endogenous metabolic pathways.MethodsHematoxylin and eosin staining and immunohistochemistry staining were performed to find pathological changes. Biochemical indicators were also measured, one-way analysis of variance with Dunnett’s multiple comparison test was used for biochemical indicators comparison among various groups. Metabolomics analysis based on ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-Q/TOF-MS) was performed to find the changes in metabolic biomarkers. Multivariate statistical approaches such as principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA) were applied to reveal group clustering trend, evaluate and maximize the discrimination between the two groups. MetaboAnalyst 4.0 was performed to find and confirm the pathways.ResultsPMR extracts exhibited slight hepatotoxic effects on the liver by increasing aspartate and alanine aminotransferase levels. Twenty-nine metabolites were identified as biomarkers, belonging to five pathways, including alpha-linolenic acid metabolism, taurine and hypotaurine metabolism, glycerophospholipid metabolism, arginine and proline metabolism, and primary bile acid biosynthesis.ConclusionThis study provided a comprehensive description of metabolomic changes between PMR- and PMRP-treated rats. The underlying mechanisms require further research.

Protective Effect of Processed Polygoni multiflori Radix and Its Major Substance during Scopolamine-Induced Cognitive Dysfunction

“…These compounds are extensively assumed to be responsible for the bioactivities of PPM. Numerous studies in the field of pharmacology have verified that PPM and its active constituents could enhance immune functions and elicit anti-oxidative and anti-ageing activities [27]. Among the active constituents of PPM, TSG has been shown to cross the blood-brain barrier and has protective effects on hippocampal synaptic plasticity [28].…”

Revive Herbal Capsule: A Review on the Mechanism of Action, Constituent Herbs and Phytochemicals

“…The iraw iRadix iPolygoni imultiflori iis isaid ito ipossess ianti-toxicity iproperty, treats carbuncles, iand ihelps iin ithe irelaxation iof ithe ilarge iintestine [39]. iOn ithe other ihand, ithe iprocessed iform iof ithis iplant iameliorates ior iprotects ithe iliver iand kidneys, icolors ithe ihair iblack iand ihelps ito imake ithe itendons iand ibones istrong.…”

A Serum Metabolomic Study on Rats Induced by Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata by Pattern Recognition and Pathways Analysis

“…After 1 week acclimation period, rats were randomly divided into three groups, with five rats each, Control group, PMR group and PMRP group. Rats were all administered 1920 mg/kg/d orally for 28 d. 28) Sample Preparation After 28 d administrated, all rats were bled through the orbital iliac vein. After an allotted clotting time of the plasma solution of 10 min, all plasma samples were centrifuged at 3000 rpm for 10 min.…”