Metabolomics Predicts Neuroimaging Characteristics of Transient Ischemic Attack Patients

Purroy, Francisco; Cambray, Serafí; Mauri-Capdevila, Gerard; Jové, Mariona; Sanahuja, Jordi; Farré, Joan; Benabdelhak, Ikram; Molina-Seguin, Jessica; Colàs-Campàs, Laura; Begué, R.; Gil, Myung Cheol; Pamplona, Reinald; Portero‐Otín, Manuel

doi:10.1016/j.ebiom.2016.11.010

Cited by 24 publications

(9 citation statements)

References 31 publications

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“…Region‐specific metabolic alterations as studied in MCAo rats revealed that significant changes in tri‐carboxylic acid (TCA) cycle along with amino acid and nucleotide metabolism occurred specifically in whole cortex and corpus striatum, but not in hippocampus . Significant alterations in neurometabolites such as Cr, cholesterol‐related molecules, threoninyl‐threonine, N‐acetyl‐glucosamine and amino acids have been revealed in acute stroke patients . 1 H magnetic resonance spectroscopy in MCAo model elucidated alteration in metabolites earlier than onset of blood–brain barrier (BBB) damage and in another study within 6 h post‐reperfusion as assessed in cerebrospinal fluid .…”

Section: Discussionmentioning

confidence: 99%

1H NMR metabolomic profiling elucidated attenuation of neurometabolic alterations by lercanidipine in MCAo model in rats

Gupta

Sharma

Jagannathan

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Stroke is a leading cause of death and disability worldwide with limited therapeutic interventions. The current study explored proton nuclear magnetic resonance spectroscopy ( 1 H NMR)-based metabolomic approach to elucidate the effect of lercanidipine on neurometabolic alterations in transient model of ischaemic stroke in rats. Methods In the present investigation, male Wistar rats were subjected to middle cerebral artery occlusion (MCAo) for 2 h followed by reperfusion using intraluminal filament method. Rats were randomly divided into three groups as vehicle-treated sham control, vehicle-treated MCAo control and lercanidipine-treated MCAo. Vehicle or lercanidipine (0.5 mg/kg, i.p.) was administered 120 min post-reperfusion. The rat brain cortex tissues were isolated 24 h post-MCAo and were investigated by 1 H NMR spectroscopy through perchloric extraction method. Key findings A total of 23 metabolites were altered significantly after cerebral ischaemic-reperfusion injury in MCAo control as compared to sham control rats. Lercanidipine significantly reduced the levels of valine, alanine, lactate, acetate and tyrosine, while N-acetylaspartate, glutamate, glutamine, aspartate, creatine/phosphocreatine, choline, glycerophosphorylcholine, taurine, myo-inositol and adenosine di-phosphate were elevated as compared to MCAo control. Conclusions Present study illustrates effect of lercanidipine on neurometabolic alterations which might be mediated through its antioxidant, anti-inflammatory, vasodilatory and anti-apoptotic property in MCAo model of stroke.

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Section: Discussionmentioning

confidence: 99%

1H NMR metabolomic profiling elucidated attenuation of neurometabolic alterations by lercanidipine in MCAo model in rats

Gupta

Sharma

Jagannathan

et al. 2020

Journal of Pharmacy and Pharmacology

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“…Those patients without contraindications were evaluated by cranial MRI that included diffusion-weighted imaging (DWI) sequences within 7 days of symptom onset. Presence of DWI abnormalities and patterns of acute ischemic lesions were also recorded ( 17 , 28 ).…”

Section: Methodsmentioning

confidence: 99%

Risk of New-Diagnosed Atrial Fibrillation After Transient Ischemic Attack

et al. 2022

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BackgroundTransient ischemic attack (TIA) provides a unique opportunity to optimize secondary preventive treatments to avoid subsequent ischemic stroke (SIS). Although atrial fibrillation (AF) is the leading cause of cardioembolism in IS and anticoagulation prevents stroke recurrence (SR), limited data exists about the risk of new-diagnosed AF (NDAF) after TIA and the consequences of the diagnostic delay. The aim of our study was to determine this risk in a cohort of TIA patients with long-term follow-up.MethodsWe carried out a prospective cohort study of 723 consecutive TIA patients from January 2006 to June 2010. Median follow-up was 6.5 (5.0–9.6) years. In a subgroup of 204 (28.2%) consecutive patients, a panel of biomarkers was assessed during the first 24 h of the onset of symptoms. Multivariate analyses were performed to find out the associated factors of NDAF. Kaplan-Meier analysis was also performed to analyzed risk of SIS.ResultsNDAF was indentified in 116 (16.0%) patients: 42 (36.2%) during admission, 18 (15.5%) within first year, 29 (25%) between one and five years and 27 (23.3%) beyond 5 years. NDAF was associated with sex (female) [hazard ratio (HR) 1.61 (95% CI, 1.07- 2.41)], age [[HR 1.05 (95% CI, 1.03–1.07)], previous ischemic heart disease (IHD) [HR 1.84, (95% CI 1.15–2.97)] and cortical DWI pattern [HR 2.81 (95% CI, 1.87–4.21)]. In the Kaplan-Meier analysis, NT-proBNP ≥ 218.2 pg/ml (log-rank test P < 0.001) was associated with significant risk of NDAF during the first 5 years of follow-up. Patients with NDAF after admission and before 5 years of follow-up had the highest risk of SIS (P = 0.002).ConclusionThe risk of NDAF after TIA is clinically relevant. We identified clinical and neuroimaging factors of NDAF. In addition, NT-proBNP was related to NDAF. Our results can be used to evaluate the benefit of long-term cardiac monitoring in selected patients.

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Section: Methodsmentioning

confidence: 99%

Early and long-term risk of new-onset atrial fibrillation after transient ischemic attack

Purroy

Vicente-Pascual

Arqué

et al. 2021

Preprint

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Background: Transient ischemic attack (TIA) provides a unique opportunity to optimize secondary preventive treatments to avoid subsequent ischemic stroke (IS). Although atrial fibrillation (AF) is the leading cause of cardioembolism in IS and anticoagulation prevents stroke recurrence (SR), limited data exists about the risk of new-onset AF after TIA. Methods: We carried out a prospective cohort study of consecutive patients with TIA from January 2006 to June 2010. The risk of new diagnosis of AF and SR was defined after a median follow-up time of 6.5 (5.0-9.6) years. In a subgroup of consecutive patients, a panel of biomarkers was assessed during the first 24 h of the onset of symptoms. Results: 723 TIA patients were included. New-onset AF was diagnosed in 116 (16.0%) patients, 42 (36.2%) of them during admission. 204 (28.2%) patients were included in the biomarker substudy. New-onset AF was associated with sex (female) (hazard ratio [HR] 1.61 [95% CI, 1.07- 2.41]), age (HR 1.05 [95% CI, 1.03-1.07]), previous ischemic heart disease (HR 1.84 [95% CI 1.15-2.97]) and cortical DWI pattern (HR 2.81 [95% CI 1.87-4.21]). In the Kaplan-Meier analysis, NT-proBNP ≥218.2 pg/ml (log-rank test P<0.001) was associated with significant risk of new-onset AF during the first five years of follow-up. Patients with a new diagnosis of AF after admission and before five years of follow-up had the highest risk of SR (P=0.002). Conclusion: The risk of new diagnosis of AF after TIA is clinically relevant. We identified clinical, neuroimaging and biomarker predictors of new-onset AF.

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Metabolomics Predicts Neuroimaging Characteristics of Transient Ischemic Attack Patients

Cited by 24 publications

References 31 publications

1H NMR metabolomic profiling elucidated attenuation of neurometabolic alterations by lercanidipine in MCAo model in rats

1H NMR metabolomic profiling elucidated attenuation of neurometabolic alterations by lercanidipine in MCAo model in rats

Risk of New-Diagnosed Atrial Fibrillation After Transient Ischemic Attack

Early and long-term risk of new-onset atrial fibrillation after transient ischemic attack

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